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AB241110

Anti-GST antibody [3E2]

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Mouse Monoclonal GST antibody. Suitable for WB and reacts with Tag samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Schistosoma japonicum Glutathione S-transferase class-mu 26 kDa isozyme.

대체 명칭 보기

Glutathione S-transferase class-mu 26 kDa isozyme, GST 26, Sj26 antigen, SjGST

1 이미지
Western blot - Anti-GST antibody [3E2] (AB241110)
  • WB

Supplier Data

Western blot - Anti-GST antibody [3E2] (AB241110)

Block : 5% Skimmed milk powder in TBST.

All lanes:

Western blot - Anti-GST antibody [3E2] (ab241110) at 1/1000 dilution

Lane 1:

Purified Pseudomonas aeruginosa Metallothionein (PmtA) GST tagged protein at 1 µg

Lane 2:

GST at 1 µg

Secondary

All lanes:

Goat anti-Mouse Ig-HRP for 1 hour at RT at 1/5000 dilution

Predicted band size: 25 kDa

true

Exposure time: 5min

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

3E2

Isotype

IgG1

Carrier free

No

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Schistosoma japonicum Glutathione S-transferase class-mu 26 kDa isozyme. The exact immunogen used to generate this antibody is proprietary information.

P08515

Specificity

Detects 26 kDa for purified GST protein. Does not detect proteins without a GST tag.

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대체품

Primary Antibodies

AB3416

HRP Anti-GST antibody

ELISA - HRP Anti-GST antibody (AB3416)

  • 5
  • 3
Primary Antibodies

AB9085

Anti-GST antibody

Lab Essentials

Western blot - Anti-GST antibody (AB9085)

  • 5
  • 2
Primary Antibodies

AB92

Anti-GST antibody [3G10/1B3]

Lab Essentials

Western blot - Anti-GST antibody [3G10/1B3] (AB92)

  • 5
  • 3
Primary Antibodies

AB111947

Anti-GST antibody [EPR4236]

20ul selling size|RabMAb|Recombinant|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GST antibody [EPR4236] (AB111947)

  • 0
  • 0
Primary Antibodies

AB235384

Anti-GST antibody [EPR4236] - BSA and Azide free

RabMAb|Recombinant

Immunoprecipitation - Anti-GST antibody [EPR4236] - BSA and Azide free (AB235384)

  • 0
  • 0

보완 제품

Primary Antibodies

AB18184

Anti-6X His tag® antibody [HIS.H8]

Lab Essentials

Western blot - Anti-6X His tag® antibody [HIS.H8] (AB18184)

  • 5
  • 32
Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

  • 5
  • 24
Primary Antibodies

AB205606

Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Western blot - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (AB205606)

  • 5
  • 2
Primary Antibodies

AB131272

Anti-ACTL6A antibody [EPR7443]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTL6A antibody [EPR7443] (AB131272)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein G
Purification 관련 사항
Purified from TCS.
보관 버퍼
Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

GST or Glutathione S-transferase is an enzyme family known for detoxifying endogenous and exogenous compounds through conjugation with glutathione. GSTs are about 23–28 kDa in size and are expressed in various tissues such as the liver kidneys and lungs. The GST proteins play vital roles in cellular processes and are utilized extensively in molecular biology often tagged to proteins to enhance solubility known as GST-tagged proteins.
Biological function summary

GST enzymes participate in critical cellular detoxification processes enhancing the solubility of toxins. As part of these processes GST enzymes do not function as part of a larger complex but are individual actors within the cell. They facilitate the conjugation reactions fundamental to cellular metabolism and detoxification making them essential for maintaining cellular health.

Pathways

GST enzymes contribute significantly to the metabolic breakdown pathways like the xenobiotic metabolism pathway. This pathway involves proteins such as cytochrome P450 enzymes which often function upstream to modify compounds for further GST action. GSTs are important for efficiently detoxifying potentially harmful substances preventing cellular damage.

GSTs have considerable relevance to cancer where their overexpression can confer drug resistance in tumor cells complicating treatment approaches. Additionally these enzymes connect with proteins such as MDR1 influencing multidrug resistance in cancer therapy. GSTs also relate to asthma where altered GST activity can affect the body's response to oxidative stress highlighting the enzyme's role in inflammatory processes.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.. GST isoenzymes appear to play a central role in the parasite detoxification system. Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut.
See full target information Glutathione S-transferase class-mu 26 kDa isozyme
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

NPJ vaccines 7:24 PubMed35197485

2022

An adjuvanted subunit SARS-CoV-2 spike protein vaccine provides protection against Covid-19 infection and transmission.

Applications

Unspecified application

Species

Unspecified reactive species

Kairat Tabynov,Nurkeldi Turebekov,Meruert Babayeva,Gleb Fomin,Toktassyn Yerubayev,Tlektes Yespolov,Lei Li,Gourapura J Renukaradhya,Nikolai Petrovsky,Kaissar Tabynov
제품이 사용된 논문 모두 보기
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Product promise

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