AB92

Anti-GST antibody [3G10/1B3]

Name: Anti-GST antibody [3G10/1B3]
Brand: Abcam Limited
SKU: AB92
Rating: 5 (3 reviews)

Lab Essentials
각 태그의 뜻

(3 리뷰들)

(42 제품이 사용된 논문 )

Mouse Monoclonal GST antibody. Carrier free. Suitable for IP, WB and reacts with Tag samples. Cited in 42 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Schistosoma japonicum Glutathione S-transferase class-mu 26 kDa isozyme.

4 이미지

Lab

Western blot - Anti-GST antibody [3G10/1B3] (AB92)

Western blot : Anti-FOXA2 antibody [EPR22919-71] - ChIP Grade ab256493 staining at 1/1000 dilution, shown in green; anti-GST antibody [3G10/1B3] ab92 staining at 1 g/mL, shown in magenta. A band was observed at 78 kDa. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween 20 (TBS-T) before incubation with primary antibodies overnight at 4 C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-FOXA2 antibody [EPR22919-71] - ChIP Grade (<a href='/ko/products/primary-antibodies/foxa2-antibody-epr22919-71-chip-grade-ab256493'>ab256493</a>) at 1/1000 dilution

Lane 1:

FOXA1 Recombinant Protein (GST Tag) at 0.1 µg

Lane 2:

FOXA2 Recombinant Protein (GST Tag) at 0.1 µg

Lane 3:

FOXA3 Recombinant Protein (GST Tag) at 0.1 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 78 kDa

Observed band size: 78 kDa

false

Lab

Western blot - Anti-GST antibody [3G10/1B3] (AB92)

Western blot : Anti-FOXA2 antibody [EPR4466] ab108422 staining at 1/1000 dilution, shown in green; [LOADING CONTROL] loading control staining at 1/[DILUTION] dilution, shown in magenta. A band was observed at 78 kDa. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween 20 (TBS-T) before incubation with primary antibodies overnight at 4 C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-FOXA2 antibody [EPR4466] (<a href='/ko/products/primary-antibodies/foxa2-antibody-epr4466-ab108422'>ab108422</a>) at 1/1000 dilution

Lane 1:

FOXA1 Recombinant Protein (GST Tag) at 0.1 µg

Lane 2:

FOXA2 Recombinant Protein (GST Tag) at 0.1 µg

Lane 3:

FOXA3 Recombinant Protein (GST Tag) at 0.1 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 78 kDa

Observed band size: 78 kDa

false

Lab

Western blot - Anti-GST antibody [3G10/1B3] (AB92)

Western blot : Anti-FOXA1 antibody ab23738 staining at 0.7 µg/mL, shown in green; Mouse anti GST ab92 loading control staining at 1/1000 dilution, shown in magenta. A band was observed at 76 kDa. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-FOXA1 + FOXA2 antibody (<a href='/ko/products/primary-antibodies/foxa1-antibody-ab23738'>ab23738</a>) at 0.7 µg/mL

Lane 1:

FOXA1 Recombinant Protein at 0.1 µg

Lane 2:

FOXA2 Recombinant Protein at 0.1 µg

Lane 3:

FOXA3 Recombinant Protein at 0.1 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 52 kDa

Observed band size: 76 kDa

false

Lab

Western blot - Anti-GST antibody [3G10/1B3] (AB92)

Anti-FOXA1 + FOXA2 + FOXA3 antibody [RM1119] ab317046 staining at 1/1000 dilution, shown in green; Mouse anti GST (ab92) loading control staining at 1/200 dilution, shown in magenta. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-FOXA1 + FOXA2 + FOXA3 antibody [RM1119] (<a href='/ko/products/primary-antibodies/foxa1-antibody-rm1119-ab317046'>ab317046</a>) at 1/1000 dilution

Lane 1:

FOXA1 Recombinant Protein (GST Tag) at 0.1 µg

Lane 2:

FOXA2 Recombinant Protein (GST Tag) at 0.1 µg

Lane 3:

FOXA3 Recombinant Protein (GST Tag) at 0.1 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 52 kDa,50 kDa,37 kDa

Observed band size: 78 kDa,76 kDa,63 kDa

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

3G10/1B3

Isotype

IgG1

Light chain 타입

kappa

Carrier free

Yes

Applications

WB, IP

applications

Immunogen

Recombinant Full Length Protein corresponding to Schistosoma japonicum Glutathione S-transferase class-mu 26 kDa isozyme.

P08515

Specificity

Recognizes Glutathione S-Transferase (GST), a 26-kDa protein encoded by the parasitic helminth Schistosoma japonicum.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-2 µg/mL", "WB-species-notes": "<p></p>" } } }

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제품 세부 정보

This product was changed from ascites to tissue culture supernatant on 25^th May 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

보관 버퍼

pH: 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

GST or Glutathione S-transferase is an enzyme family known for detoxifying endogenous and exogenous compounds through conjugation with glutathione. GSTs are about 23–28 kDa in size and are expressed in various tissues such as the liver kidneys and lungs. The GST proteins play vital roles in cellular processes and are utilized extensively in molecular biology often tagged to proteins to enhance solubility known as GST-tagged proteins.

Biological function summary

GST enzymes participate in critical cellular detoxification processes enhancing the solubility of toxins. As part of these processes GST enzymes do not function as part of a larger complex but are individual actors within the cell. They facilitate the conjugation reactions fundamental to cellular metabolism and detoxification making them essential for maintaining cellular health.

Pathways

GST enzymes contribute significantly to the metabolic breakdown pathways like the xenobiotic metabolism pathway. This pathway involves proteins such as cytochrome P450 enzymes which often function upstream to modify compounds for further GST action. GSTs are important for efficiently detoxifying potentially harmful substances preventing cellular damage.

GSTs have considerable relevance to cancer where their overexpression can confer drug resistance in tumor cells complicating treatment approaches. Additionally these enzymes connect with proteins such as MDR1 influencing multidrug resistance in cancer therapy. GSTs also relate to asthma where altered GST activity can affect the body's response to oxidative stress highlighting the enzyme's role in inflammatory processes.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.. GST isoenzymes appear to play a central role in the parasite detoxification system. Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut.

See full target information Glutathione S-transferase class-mu 26 kDa isozyme

대체 명칭 보기

Glutathione S-transferase class-mu 26 kDa isozyme, GST 26, Sj26 antigen, SjGST

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제품이 사용된 논문 (42)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature communications 16:5892 PubMed40603292

2025

Natural variation in GNP3 determines grain number and grain yield in rice.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiqi Ma,Yuhang Ming,Jinlong Li,Honglin Li,Haozhen Wang,Xiaoyang Zhu,Yawen Xu,Yong Zhao,Qianfeng Hu,Ruiqi Liu,Yinghua Pan,Danting Li,Wensheng Wang,Jianlong Xu,Xingming Sun,Jinjie Li,Hongliang Zhang,Zichao Li,Zhanying Zhang

The New phytologist 245:2726-2743 PubMed39887382

2025

GS2 cooperates with IPA1 to control panicle architecture.

Applications

Unspecified application

Species

Unspecified reactive species

Yueying Wang,Yang Lv,Yi Wen,Junge Wang,Peng Hu,Kaixiong Wu,Bingze Chai,Shuxian Gan,Jialong Liu,Yue Wu,Lixin Zhu,Nannan Dong,Yiqing Tan,Hao Wu,Guangheng Zhang,Li Zhu,Deyong Ren,Qiang Zhang,Yuexing Wang,Qian Qian,Jiang Hu

Nature communications 15:9689 PubMed39516491

2024

HURP facilitates spindle assembly by stabilizing microtubules and working synergistically with TPX2.

Applications

Unspecified application

Species

Unspecified reactive species

Venecia Alexandria Valdez,Meisheng Ma,Bernardo Gouveia,Rui Zhang,Sabine Petry

The Journal of cell biology 223: PubMed39514288

2024

BEACH domain proteins function as cargo-sorting adaptors in secretory and endocytic pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Serhiy Pankiv,Anette Kathinka Dahl,Aleksander Aas,Rosa Linn Andersen,Andreas Brech,Petter Holland,Sakshi Singh,Christian Bindesbøll,Anne Simonsen

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2405459 PubMed39206796

2024

Genomic Amplification of TBC1D31 Promotes Hepatocellular Carcinoma Through Reducing the Rab22A-Mediated Endolysosomal Trafficking and Degradation of EGFR.

Applications

Unspecified application

Species

Unspecified reactive species

Pengbo Cao,Hongxia Chen,Ying Zhang,Qi Zhang,Mengting Shi,Huihui Han,Xiaowen Wang,Liang Jin,Bingqian Guo,Rongjiao Hao,Xi Zhao,Yuanfeng Li,Chengming Gao,Xinyi Liu,Yahui Wang,Aiqing Yang,Chenning Yang,Anfeng Si,Hua Li,Qingfeng Song,Fuchu He,Gangqiao Zhou

Frontiers in immunology 15:1379175 PubMed39086481

2024

Characterization of latently infected EBV+ antibody-secreting B cells isolated from ovarian tumors and malignant ascites.

Applications

Unspecified application

Species

Unspecified reactive species

Lixin Zhang,Mary Strange,Esther Elishaev,Syed Zaidi,Francesmary Modugno,Mackenzy Radolec,Robert P Edwards,Olivera J Finn,Anda M Vlad

Cell genomics 4:100550 PubMed38697125

2024

NRDE2 deficiency impairs homologous recombination repair and sensitizes hepatocellular carcinoma to PARP inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Yahui Wang,Xinyi Liu,Xianbo Zuo,Cuiling Wang,Zheng Zhang,Haitao Zhang,Tao Zeng,Shunqi Chen,Mengyu Liu,Hongxia Chen,Qingfeng Song,Qi Li,Chenning Yang,Yi Le,Jinliang Xing,Hongxin Zhang,Jiaze An,Weihua Jia,Longli Kang,Hongxing Zhang,Hui Xie,Jiazhou Ye,Tianzhun Wu,Fuchu He,Xuejun Zhang,Yuanfeng Li,Gangqiao Zhou

Nature communications 14:7838 PubMed38030598

2023

The protein interactome of the citrus Huanglongbing pathogen Candidatus Liberibacter asiaticus.

Applications

Unspecified application

Species

Unspecified reactive species

Erica W Carter,Orlene Guerra Peraza,Nian Wang

Plant communications 5:100673 PubMed37596786

2023

GR5 acts in the G protein pathway to regulate grain size in rice.

Applications

Unspecified application

Species

Unspecified reactive species

Yueying Wang,Yang Lv,Haiping Yu,Peng Hu,Yi Wen,Junge Wang,Yiqing Tan,Hao Wu,Lixin Zhu,Kaixiong Wu,Bingze Chai,Jialong Liu,Dali Zeng,Guangheng Zhang,Li Zhu,Zhenyu Gao,Guojun Dong,Deyong Ren,Lan Shen,Qiang Zhang,Qing Li,Longbiao Guo,Guosheng Xiong,Qian Qian,Jiang Hu

Insect science 30:1607-1621 PubMed36915030

2023

Methyl-CpG binding domain (MBD)2/3 specifically recognizes and binds to the genomic mCpG site with a β-sheet in the MBD to affect embryonic development in Bombyx mori.

Applications

Unspecified application

Species

Unspecified reactive species

Tong-Yu Fu,Shuang-Shun Ji,Yu-Lin Tian,Yi-Guang Lin,Yu-Mei Chen,Qi-En Zhong,Si-Chun Zheng,Guan-Feng Xu

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GST antibody [3G10/1B3]

Western blot - Anti-GST antibody [3G10/1B3] (AB92)

Western blot - Anti-GST antibody [3G10/1B3] (AB92)

Western blot - Anti-GST antibody [3G10/1B3] (AB92)

Western blot - Anti-GST antibody [3G10/1B3] (AB92)

주요 정보

Host species

Clonality

Clone number

Isotype

Light chain 타입

Carrier free

Applications

Immunogen

Specificity

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (42)

Product promise