AB248729

Anti-GSTA4 antibody [EPR8404(B)] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal GSTA4 antibody. Carrier free. Suitable for WB and reacts with Human samples.

대체 명칭 보기

Glutathione S-transferase A4, GST class-alpha member 4, Glutathione S-transferase A4-4, GSTA4

1 이미지

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Western blot - Anti-GSTA4 antibody [EPR8404(B)] - BSA and Azide free (AB248729)

This data was developed using ab134919, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-GSTA4 antibody [EPR8404(B)] (<a href='/ko/products/primary-antibodies/gsta4-antibody-epr8404b-ab134919'>ab134919</a>) at 1/1000 dilution

Lane 1:

Human fetal brain lysate at 10 µg

Lane 2:

Human fetal liver lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Lane 4:

HepG2 cell lysate at 10 µg

Lane 5:

JAR cell lysate at 10 µg

Secondary

All lanes:

Goat-anti-rabbit HRP at 1/2000 dilution

Predicted band size: 26 kDa

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주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8404(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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제품 세부 정보

ab248729 is the carrier-free version of ab134919.

Species reactivity
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

GSTA4 also known as Glutathione S-transferase alpha-4 is an enzyme that plays a critical role in detoxification processes. This protein weighs approximately 25 kDa and exhibits strong expression in tissues such as the liver kidney and brain. GSTA4 belongs to the glutathione S-transferase superfamily specifically the alpha class which is involved in the conjugation of the reduced form of glutathione (GSH) to a wide range of hydrophobic electrophiles.

Biological function summary

GSTA4 helps in the metabolism of lipid peroxidation products by catalyzing their conjugation with glutathione. This process is essential for reducing oxidative stress caused by cellular metabolism. GSTA4 does not normally function as part of a larger complex but instead works independently to carry out this detoxification role. The ability of GSTA4 to target lipid peroxidation products is significant for cellular protection mechanisms.

Pathways

GSTA4 actively participates in the detoxification pathway where it neutralizes harmful compounds like 4-hydroxynonenal (4-HNE) a byproduct of lipid peroxidation. GSTA4 is also involved in the glutathione metabolism pathway playing an important role alongside other GST isoforms in managing oxidative stress within cells. Proteins related through these pathways include other members of the glutathione S-transferase family which share similar functional roles in detoxification processes.

GSTA4 impacts the development of conditions related to oxidative stress such as Alzheimer's disease and cancer. Through its detoxifying activity GSTA4 helps mitigate oxidative damage that may contribute to the progression of these diseases. Research indicates the dysregulation of GSTA4 expression links to both Alzheimer's disease and cancer along with its interactions with other GST family proteins highlighting its importance in disease pathology.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. This isozyme has a high catalytic efficiency with 4-hydroxyalkenals such as 4-hydroxynonenal (4-HNE).

See full target information Glutathione S-transferase A4

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GSTA4 antibody [EPR8404(B)] - BSA and Azide free

Western blot - Anti-GSTA4 antibody [EPR8404(B)] - BSA and Azide free (AB248729)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Proteins & Peptides

Primary Antibodies

Proteins & Peptides

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

Product promise