Anti-GSTM3 antibody

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSTM3 antibody (AB272613)

Paraffin-embedded formalin-fixed Human testis tissue stained for GSTM3 using ab272613 at 1/500 in immunohistochemical analysis.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSTM3 antibody (AB272613)

Paraffin-embedded formalin-fixed Human lymph tissue stained for GSTM3 using ab272613 at 1/500 in immunohistochemical analysis. Low expression as expected.

• WB

Unknown

Western blot - Anti-GSTM3 antibody (AB272613)

Lane 1:

Western blot - Anti-GSTM3 antibody (ab272613)

Lane 2:

Western blot - Anti-GSTM3 antibody (ab272613) at 0.4 µg/mL

Lane 1:

Molecular weight ladder

Lane 2:

Human cell line RT4

Predicted band size: 27 kDa

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• WB

Lab

Western blot - Anti-GSTM3 antibody (AB272613)

Western blot : Anti-GSTM3 antibody (ab272613) staining at 0.4 ug/ml, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab272613 was shown to bind specifically to GSTM3. A band was observed at 25 kDa in wild-type A549 cell lysates with no signal observed at this size in GSTM3 knockout cell line. To generate this image, wild-type and GSTM3 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-GSTM3 antibody (ab272613) at 0.4 µg/mL

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

GSTM3 knockout A549 cell lysate at 20 µg

Lane 3:

MCF7 cell lysate at 20 µg

Lane 4:

HCT 116 cell lysate at 20 µg

Secondary

Lanes 1 - 4:

Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution

Lanes 1 - 4:

Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 25 kDa

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