AB86387

Anti-GWL antibody

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(9 제품이 사용된 논문 )

Rabbit Polyclonal GWL antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human MASTL aa 550 to C-terminus.

3 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GWL antibody (AB86387)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GWL antibody (AB86387)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma (left) and lung carcinoma (right) tissues labelling MASTL with ab86387 at 1/1000 (1µg/ml) and 1/500 (2µg/ml). Detection : DAB and a DyLight® 594-conjugated goat anti-rabbit IgG (H+L) (1/100).

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Immunoprecipitation - Anti-GWL antibody (AB86387)

Detection of GWL by Western blot of Immunprecipitate.
ab86387, at 1 μg/ml, staining GWL in HeLa whole cell lysate immunoprecipitated using ab86387 at 3 μg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-GWL antibody (ab86387)

Predicted band size: 97 kDa

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Western blot - Anti-GWL antibody (AB86387)

All lanes:

Western blot - Anti-GWL antibody (ab86387) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 97 kDa

Observed band size: 118 kDa

true

Exposure time: 3min

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human MASTL aa 550 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q96GX5

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

GWL also known as Greatwall kinase (and occasionally Mastl) is a protein kinase with a calculated molecular mass of about 58 kDa. This protein mainly expresses in various tissues but it shows higher expression in organs such as the heart liver and skeletal muscle. GWL plays a mechanical role in the regulation of cell cycle through its kinase activity which phosphorylates other key proteins involved in mitosis.

Biological function summary

GWL influences the cell cycle and mitotic progression by modulating the activity of the phosphatase inhibitor proteins ENSA and ARPP19. It is part of a functional complex with these proteins when activated leading to the inhibition of Protein Phosphatase 2A (PP2A) during mitosis which allows for proper chromosome condensation and segregation. This ensures that the cells divide accurately maintaining genomic stability.

Pathways

Different cellular pathways integrate their signals through GWL to regulate mitosis. One of the key pathways is the cell cycle control pathway where GWL works alongside CDK1 and is essential for the phosphorylation cascade that leads to mitotic entry. Another pathway of note is the mitotic spindle assembly checkpoint where GWL interacts with proteins such as BUB1 and MAD2 helping establish mechanisms that ensure cells do not prematurely progress through mitosis with errors.

GWL dysfunction links to cancer due to its critical role in cell division. Aberrant expression or mutation of GWL can lead to aneuploidy and genomic instability factors that contribute to tumorigenesis. Moreover abnormalities in GWL expression have been associated with neurological disorders like Alzheimer's disease where it may interact with tau protein aggregation pathways. In cancer GWL often works in conjunction with oncogenes like MYC and tumor suppressors such as p53 influencing their roles in controlling cell proliferation and survival.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Serine/threonine kinase that plays a key role in M phase by acting as a regulator of mitosis entry and maintenance (PubMed : 19680222). Acts by promoting the inactivation of protein phosphatase 2A (PP2A) during M phase : does not directly inhibit PP2A but acts by mediating phosphorylation and subsequent activation of ARPP19 and ENSA at 'Ser-62' and 'Ser-67', respectively (PubMed : 38123684). ARPP19 and ENSA are phosphatase inhibitors that specifically inhibit the PPP2R2D (PR55-delta) subunit of PP2A. Inactivation of PP2A during M phase is essential to keep cyclin-B1-CDK1 activity high (PubMed : 20818157). Following DNA damage, it is also involved in checkpoint recovery by being inhibited. Phosphorylates histone protein in vitro; however such activity is unsure in vivo. May be involved in megakaryocyte differentiation.

See full target information MASTL

대체 명칭 보기

GW, GWL, THC2, MASTL, Serine/threonine-protein kinase greatwall, hGWL, Microtubule-associated serine/threonine-protein kinase-like, MAST-L

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제품이 사용된 논문 (9)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell reports. Medicine 4:100937 PubMed36787737

2023

Harnessing transcriptionally driven chromosomal instability adaptation to target therapy-refractory lethal prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Brittiny Dhital,Sandra Santasusagna,Perumalraja Kirthika,Michael Xu,Peiyao Li,Marc Carceles-Cordon,Rajesh K Soni,Zhuoning Li,Ronald C Hendrickson,Matthew J Schiewer,William K Kelly,Cora N Sternberg,Jun Luo,Amaia Lujambio,Carlos Cordon-Cardo,Monica Alvarez-Fernandez,Marcos Malumbres,Haojie Huang,Adam Ertel,Josep Domingo-Domenech,Veronica Rodriguez-Bravo

iScience 25:104459 PubMed35677646

2022

MASTL is enriched in cancerous and pluripotent stem cells and influences OCT1/OCT4 levels.

Applications

Unspecified application

Species

Unspecified reactive species

Elisa Närvä,Maria E Taskinen,Sergio Lilla,Aleksi Isomursu,Mika Pietilä,Jere Weltner,Jorma Isola,Harri Sihto,Heikki Joensuu,Sara Zanivan,Jim Norman,Johanna Ivaska

The Journal of cell biology 219: PubMed32311005

2020

MASTL promotes cell contractility and motility through kinase-independent signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Emilia Taskinen,Elisa Närvä,James R W Conway,Laura Soto Hinojosa,Sergio Lilla,Anja Mai,Nicola De Franceschi,Laura L Elo,Robert Grosse,Sara Zanivan,Jim C Norman,Johanna Ivaska

Cancers 11: PubMed31200459

2019

Wee1 Rather Than Plk1 Is Inhibited by AZD1775 at Therapeutically Relevant Concentrations.

Applications

Unspecified application

Species

Unspecified reactive species

Angela Flavia Serpico,Giuseppe D'Alterio,Cinzia Vetrei,Rosa Della Monica,Luca Nardella,Roberta Visconti,Domenico Grieco

Oncology letters 17:3163-3172 PubMed30867746

2019

Inflammatory cytokine-induced expression of MASTL is involved in hepatocarcinogenesis by regulating cell cycle progression.

Applications

Unspecified application

Species

Unspecified reactive species

Liye Cao,Wen-Juan Li,Ji-Hong Yang,Yu Wang,Zhi-Juan Hua,Dan Liu,Ya-Qing Chen,Hao-Miao Zhang,Rui Zhang,Ji-Sen Zhao,Shu-Jie Cheng,Quan Zhang

Cancer letters 442:362-372 PubMed30445205

2018

Mitosis perturbation by MASTL depletion impairs the viability of thyroid tumor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Elena Cetti,Tiziana Di Marco,Giuseppe Mauro,Mara Mazzoni,Daniele Lecis,Emanuela Minna,Lucia Gioiosa,Silvia Brich,Sonia Pagliardini,Maria Grazia Borrello,Giancarlo Pruneri,Maria Chiara Anania,Angela Greco

Oncotarget 6:34629-48 PubMed26431489

2015

Identification of thyroid tumor cell vulnerabilities through a siRNA-based functional screening.

Applications

Species

Unspecified reactive species

Maria Anania,Fabio Gasparri,Elena Cetti,Ivan Fraietta,Katia Todoerti,Claudia Miranda,Mara Mazzoni,Claudia Re,Riccardo Colombo,Giorgio Ukmar,Stefano Camisasca,Sonia Pagliardini,Marco Pierotti,Antonino Neri,Arturo Galvani,Angela Greco

Biochemical and biophysical research communications 442:56-61 PubMed24211627

2013

ENSA expression correlates with attenuated tumor propagation in liver cancer.

Applications

Species

Human

Yao-Li Chen,Ming-Han Kuo,Ping-Yi Lin,Wan-Ling Chuang,Chia-Chen Hsu,Pei-Yi Chu,Chia-Huei Lee,Tim H-M Huang,Yu-Wei Leu,Shu-Huei Hsiao

Molecular biology of the cell 22:1191-206 PubMed21325631

2011

Mitotic progression becomes irreversible in prometaphase and collapses when Wee1 and Cdc25 are inhibited.

Applications

Species

Human

Tamara A Potapova,Sushama Sivakumar,Jennifer N Flynn,Rong Li,Gary J Gorbsky

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GWL antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GWL antibody (AB86387)

Immunoprecipitation - Anti-GWL antibody (AB86387)

Western blot - Anti-GWL antibody (AB86387)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (9)

Product promise