AB215992

Anti-H2R antibody

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Rabbit Polyclonal H2R antibody. Suitable for IHC-P and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human Histamine H2 receptor aa 150 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

대체 명칭 보기

Histamine H2 receptor, H2R, HH2R, Gastric receptor I, HRH2

2 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H2R antibody (AB215992)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H2R antibody (AB215992)

Immunohistochemical analysis of formalin-fixed and paraffin-embedded mouse stomach tissue labeling H2R with ab215992 at 1/200 dilution, followed by secondary antibody (Goat Anti-Rabbit IgG, Cy3 conjugated) at 1/200 dilution and DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H2R antibody (AB215992)

Immunohistochemical analysis of formalin-fixed and paraffin-embedded mouse stomach tissue labeling H2R with ab215992 at 1/200 dilution, followed by secondary antibody and DAB staining.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human Histamine H2 receptor aa 150 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P25021

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>When using a fluorescent probe the recommended dilution is 1/50 – 1/200.</p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

H2R also known as histamine H2 receptor is a G-protein-coupled receptor with a mass of approximately 40 kDa. It is expressed mainly in the gastric parietal cells as well as in the heart brain and various immune cells. H2R modulates gastric acid secretion by engaging in the interaction with histamine which activates the receptor. This activation includes changes in intracellular cyclic AMP levels and subsequent cellular responses.

Biological function summary

The histamine H2 receptor plays a significant role in the mediation of gastric acid secretion cardiac muscle contraction and immune response modulation. Although it functions independently it does interact with other receptors giving a context like mast cells which secrete histamine. These receptors play an important role in stimulating gastric parietal cells to produce acid as well as having involvement in the modulation of heart rate and force of contraction.

Pathways

H2R participates in the GPCR signaling pathway and adenylate cyclase activation both of which are critical for physiological responses in gastric acid secretion and modulation of cardiac function. H2R interacts with proteins such as adenylate cyclase and other H receptors like H1 receptor contributing to the regulation of varied responses across tissues like the stomach heart and brain.

H2R is strongly related to peptic ulcers and gastroesophageal reflux disease (GERD). In these conditions excessive secretion of gastric acid due to H2R overactivity can lead to mucosal damage and discomfort. Moreover other histamine receptors like H1 receptor might influence inflammatory processes and allergy drawing connections that are relevant to understanding histamine's overall impacts in the body.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

G-protein coupled receptor for histamine, primarily mediating gastric acid secretion. Predominantly expressed in the gastric mucosa, couples to G(s) G alpha proteins upon histamine binding, leading to activation of adenylate cyclase and increased intracellular cyclic AMP (cAMP) levels (PubMed : 38647423, PubMed : 39333117). This signaling cascade stimulates parietal cells to secrete hydrochloric acid, playing a key role in digestive physiology. Also expressed in other tissues, including the heart and central nervous system, where it may contribute to cardiac stimulation and modulate neurotransmitter release (By similarity).

See full target information Histamine H2 receptor

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Biomedicines 11: PubMed37189747

2023

Moderate-Intensity Exercise Improves Mesenteric Arterial Function in Male UC Davis Type-2 Diabetes Mellitus (UCD-T2DM) Rats: A Shift in the Relative Importance of Endothelium-Derived Relaxing Factors (EDRF).

Applications

Unspecified application

Species

Unspecified reactive species

Md Rahatullah Razan,Said Amissi,Rifat Ara Islam,James L Graham,Kimber L Stanhope,Peter J Havel,Roshanak Rahimian

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-H2R antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H2R antibody (AB215992)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H2R antibody (AB215992)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise