- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HA tag antibody [HL1603] (AB308398)
Immunohistochemical analysis of mock and HA tag - transfected HEK293T cell pellet labeling HA tag using ab308398 at 1/1000 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HL1603] (AB308398)
For immunofluorescence analysis, mock and transfected HEK293T cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of HA tag using ab308398 at 1/500 dilution (green). Blue : Fluoroshield with DAPI.
- I-ELISA
Supplier Data
Indirect ELISA - Anti-HA tag antibody [HL1603] (AB308398)
Indirect ELISA analysis was performed by coating the plate with recombinant HA-tag tagged protein (1000-15.63 ng/mL). Coated protein was probed with HA tag antibody [HL1603] (ab308398) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (1/10000) was used to detect the bound primary antibody.
Reactivity 정보
제품 세부 정보
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
특성 및 보관 정보
제형
Purification 테크닉
보관 버퍼
배송 시 보관 조건
적절한 단기 보관 기간
적절한 단기 보관 조건
적절한 장기 보관 조건
분주 정보
보관 정보
제품 프로토콜
- Visit the General protocols
- Visit the Troubleshooting
타겟 정보
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com