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AB240161

Anti-HAS3 antibody [EPR10397] - BSA and Azide free

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Rabbit Recombinant Monoclonal HAS3 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

대체 명칭 보기

Hyaluronan synthase 3, Hyaluronate synthase 3, Hyaluronic acid synthase 3, HA synthase 3, HAS3

1 이미지
Western blot - Anti-HAS3 antibody [EPR10397] - BSA and Azide free (AB240161)
  • WB

Supplier Data

Western blot - Anti-HAS3 antibody [EPR10397] - BSA and Azide free (AB240161)

This data was developed using ab170872, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-HAS3 antibody [EPR10397] (<a href='/ko/products/primary-antibodies/has3-antibody-epr10397-ab170872'>ab170872</a>) at 1/1000 dilution

Lane 1:

Human fetal lung lysate at 10 µg

Lane 2:

Human fetal kidney lysate at 10 µg

Lane 3:

Human uterus lysate at 10 µg

Lane 4:

Human ovary cancer lysate at 10 µg

Secondary

All lanes:

HRP labeled goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 63 kDa

false

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10397

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Primary Antibodies

AB290669

Anti-Hyaluronan synthase 2 antibody [EPR24637-109] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-Hyaluronan synthase 2 antibody [EPR24637-109] - BSA and Azide free (Detector) (AB290669)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB198846

Anti-HAS1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HAS1 antibody (AB198846)

  • 0
  • 0
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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제품 세부 정보

ab240161 is the carrier-free version of ab170872.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Do Not Freeze
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The HAS3 protein also known as hyaluronan synthase 3 is one of the enzymes responsible for the synthesis of hyaluronan a component of the extracellular matrix. HAS3 is smaller compared to other hyaluronan synthases with a mass of approximately 65 kDa. The enzyme is expressed in various tissues including the skin heart and lungs. Its regulation involves various cellular signals and it plays a role in cellular processes affecting tissue dynamics.
Biological function summary

HAS3 contributes to the regulation of extracellular matrix composition by synthesizing hyaluronan which affects cell migration and proliferation. It is not part of a classic complex but functions in coordination with other hyaluronan synthases like HAS1 and HAS2. The presence of hyaluronan influences cell behavior and tissue repair emphasizing its role in dynamic anatomical environments.

Pathways

HAS3 actively participates in the glycosaminoglycan biosynthesis pathway and extracellular matrix organization. Its activity is associated with signaling processes that include interactions with CD44 a receptor for hyaluronan which mediates various cellular responses like adhesion and migration. These pathways link HAS3 to processes involving tissue development and wound healing.

HAS3 shows connections to cancer progression and osteoarthritis. Overexpression of HAS3 has been associated with increased tumor cell motility and invasion highlighting its role in metastasis. In osteoarthritis altered hyaluronan synthesis through HAS3 activity is implicated in cartilage degradation. Its interaction with proteins such as CD44 in these conditions highlights HAS3's involvement in disease-related matrix remodeling.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Catalyzes the addition of GlcNAc or GlcUA monosaccharides to the nascent hyaluronan polymer. Therefore, it is essential to hyaluronan synthesis a major component of most extracellular matrices that has a structural role in tissues architectures and regulates cell adhesion, migration and differentiation. This is one of three isoenzymes responsible for cellular hyaluronan synthesis.
See full target information Hyaluronan synthase 3
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan 43:494-500 PubMed37147750

2023

Electroacupuncture alleviates water avoidance stress-induced irritable bowel syndrome in mice by improving intestinal barrier functions and suppressing the expression of inflammatory cytokines.

Applications

Unspecified application

Species

Unspecified reactive species

Sun Mengzhu,Zhang Yujie,Song Yafang,Guo Jing,Wang Yuhang,Xin Chen,G U Dongmei,Sun Jianhua,Pei Lixia
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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