AB66792

Anti-HBEGF/DTR antibody [4G10]

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(6 제품이 사용된 논문 )

Mouse Monoclonal HBEGF/DTR antibody. Suitable for IP, WB, ICC/IF and reacts with Transfected cell line - Human, Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HBEGF.

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Immunocytochemistry/ Immunofluorescence - Anti-HBEGF/DTR antibody [4G10] (AB66792)

ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-HBEGF/DTR antibody [4G10] (AB66792)

These images show Vero cells carrying human HG-EGF expression vector (left hand image) and Vero cells carrying mouse HBEGF/DTR expression vector (right hand image). Cells treated with ab 66792, fixed with 4% PFA and reacted with Cys3 conjugated 2nd antibody.

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Immunoprecipitation - Anti-HBEGF/DTR antibody [4G10] (AB66792)

Lane 1 : Vero cell extract, 1μg
Lane 2 : Vero cells carrying Human HBEGF/DTR expression vector, 1μg
Lane 3 : Vero Cells carrying mouse HBEGF/DTR expression vector, 1μg

ab66792 immunoprecipitating HBEGF/DTR in Vero Cells. For western blotting, a HRP conjugated streptavidin was used as the secondary antibody; cell surface was biotinylated.

Lane 1:

Immunoprecipitation - Anti-HBEGF/DTR antibody [4G10] (ab66792)

Lane 1:

Immunoprecipitation - Biotin Anti-HBEGF/DTR antibody [4G10] (<a href='/ko/products/primary-antibodies/biotin-hbegf-dtr-antibody-4g10-ab66793'>ab66793</a>)

Predicted band size: 23 kDa

Observed band size: 20-38 kDa,41 kDa

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Western blot - Anti-HBEGF/DTR antibody [4G10] (AB66792)

Lanes 1 - 3:

Western blot - Biotin Anti-HBEGF/DTR antibody [4G10] (<a href='/ko/products/primary-antibodies/biotin-hbegf-dtr-antibody-4g10-ab66793'>ab66793</a>) at 1 µg/mL

Lanes 1 - 3:

Western blot - Anti-HBEGF/DTR antibody [4G10] (ab66792) at 1 µg/mL

Lane 1:

Vero (African green monkey kidney epithelial cell) whole cell lysate

Lane 2:

Vero cell extract from cells carrying human HBEGF/DTR expression vector

Lane 3:

Vero cell extract from cells carrying mouse HBEGF/DTR expression vector

Predicted band size: 23 kDa

Observed band size: 20-28 kDa,41 kDa

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

4G10

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

WB, ICC/IF, IP

applications

Immunogen

Recombinant Fragment Protein within Human HBEGF. The exact immunogen used to generate this antibody is proprietary information.

Q99075

Epitope

EGF domain

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5-10 µg/mL", "ICCIF-species-notes": "" }, "Transfected cell line - Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "Use at 2μg/ml", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

Purification 관련 사항

Produced in serum-free medium and purified by combination of chromatography

보관 버퍼

pH: 6 - 8.5 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

HBEGF also known as Heparin-binding EGF-like growth factor or Diphtheria Toxin Receptor (DTR) is a growth factor that binds to EGF receptors with its mass approximately 20 kilodaltons. It is a member of the EGF family. Cells secrete HBEGF and it is expressed in many tissues including the heart kidney and lungs. Its expression often increases during proliferation and differentiation making it an important player in tissue growth and repair.

Biological function summary

HBEGF functions as a mitogen and chemotactic agent which means it stimulates cell division and attracts cells to migrate. As part of the EGF family HBEGF activates the EGFR (epidermal growth factor receptor) signaling cascade. This signaling is essential for processes such as wound healing and development. HBEGF also forms complexes with other ligands and receptors enhancing its biological activity in tissues.

Pathways

HBEGF plays a role in the MAPK/ERK signaling pathway and the PI3K/AKT pathway. Both pathways are significant for regulating cell growth survival and differentiation. HBEGF interacts with other proteins like Ras and PI3K in these pathways. By activating these pathways HBEGF influences cellular responses critical for normal physiological processes and recovery mechanisms in cells.

HBEGF has connections with cancer and cardiovascular diseases. Aberrant HBEGF signaling can promote oncogenic processes in cancer relating it to proteins such as HER2 which also engage in similar pathways. In cardiovascular diseases HBEGF overexpression can contribute to smooth muscle cell proliferation an important event in atherosclerosis linking it to other growth factors involved in vascular biology.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. Required for normal cardiac valve formation and normal heart function. Promotes smooth muscle cell proliferation. May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts, but not endothelial cells. It is able to bind EGF receptor/EGFR with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.

See full target information HBEGF

대체 명칭 보기

DTR, DTS, HEGFL, HBEGF, Proheparin-binding EGF-like growth factor

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제품이 사용된 논문 (6)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cancer cell international 25:219 PubMed40542386

2025

Hsa_circ_0059511 promote glioma cell proliferation and migration through hsa-miR-194-5p/HBEGF axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yajun Jing,Huang-Cheng Shang-Guan,Jiawei Cai,Jinye Su,Wei Huang,Qiu He,Xiaoyong Chen,Dezhi Kang,Zanyi Wu

Science advances 6: PubMed32917630

2020

Attenuated diphtheria toxin mediates siRNA delivery.

Applications

Unspecified application

Species

Unspecified reactive species

Amy E Arnold,Laura J Smith,Greg L Beilhartz,Laura C Bahlmann,Emma Jameson,Roman A Melnyk,Molly S Shoichet

Experimental and therapeutic medicine 13:3021-3031 PubMed28587375

2017

C-X-C motif chemokine ligand 8 promotes endothelial cell homing via the Akt-signal transducer and activator of transcription pathway to accelerate healing of ischemic and hypoxic skin ulcers.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Shen,Peng Zhang,Shanqiang Zhang,Liping Xie,Lijie Yao,Weiya Lang,Jie Lian,Wei Qin,Meng Zhang,Liang Ji

Transfusion and apheresis science : official journ 55:44-52 PubMed27470710

2016

Does smoking, age or gender affect the protein phenotype of extracellular vesicles in plasma?

Applications

Unspecified application

Species

Unspecified reactive species

R Bæk,K Varming,M M Jørgensen

Arteriosclerosis, thrombosis, and vascular biology 32:2484-92 PubMed22859491

2012

Modulation of the cochaperone AHA1 regulates heat-shock protein 90 and endothelial NO synthase activation by vascular endothelial growth factor.

Applications

Unspecified application

Species

Unspecified reactive species

Fanny Desjardins,Chantal Delisle,Jean-Philippe Gratton

Biochemical and biophysical research communication 422:676-80 PubMed22609777

2012

The anti-tumor effect of cross-reacting material 197, an inhibitor of heparin-binding EGF-like growth factor, in human resistant ovarian cancer.

Applications

Unspecified application

Species

Human

Xiao-han Tang,Suo Deng,Meng Li,Mei-song Lu

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HBEGF/DTR antibody [4G10]

Immunocytochemistry/ Immunofluorescence - Anti-HBEGF/DTR antibody [4G10] (AB66792)

Immunoprecipitation - Anti-HBEGF/DTR antibody [4G10] (AB66792)

Western blot - Anti-HBEGF/DTR antibody [4G10] (AB66792)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Epitope

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (6)

Product promise