AB35586

Anti-HCV subtype 1b NS5B antibody

Name: Anti-HCV subtype 1b NS5B antibody
Brand: Abcam Limited
SKU: AB35586
Rating: 4 (1 reviews)

(1 리뷰)

(18 제품이 사용된 논문 )

Rabbit Polyclonal POLG antibody. Suitable for ELISA, WB and reacts with Hepatitis C virus samples. Cited in 18 publications.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Hepatitis C virus

Applications

WB, ELISA

applications

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Hepatitis C virus": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

style

left-column

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

HCV subtype 1b NS5B also known as nonstructural protein 5B is an RNA-dependent RNA polymerase (RdRp) important for Hepatitis C virus replication. This enzyme is an important component of the HCV replication complex facilitating viral RNA synthesis. It has a molecular mass of approximately 66 kDa. NS5B is expressed in infected liver cells where it remains membrane-associated due to its C-terminal tail. Antibodies against HCV NS5B such as anti-HCV are often utilized in research for detection and analysis including HCV Western blot applications.

Biological function summary

This enzyme plays a critical role in the synthesis of viral RNA by using viral RNA as a template. NS5B is not part of a classical protein complex but interacts with other viral proteins in the replication complex including NS3 and NS5A to carry out RNA replication and produce progeny virus. Anti-HCV antibodies can aid in studying these interactions. Enzyme inhibitors targeting NS5B are a major focus in HCV therapeutic development efforts.

Pathways

NS5B participates in the life cycle of HCV specifically the replication phase. The replication of HCV involves multiple stages and NS5B's role is integral in the RNA synthesis step. It interacts with the NS5A protein which modulates RNA replication. The interplay between NS5B and NS5A represents a significant point in the HCV replication pathway influencing viral loads and laboratory studies often use anti-HCV and anti-HCV negative controls in mechanistic research.

HCV subtype 1b NS5B is central in chronic Hepatitis C infection and liver diseases such as cirrhosis and hepatocellular carcinoma. Interference with NS5B's RNA polymerase activity is a proven strategy in antiviral therapy making it a target for drugs like polymerase inhibitors. Its connection to NS5A which also supports viral replication corroborates its relevance in HCV pathology and targeted therapies often include combination treatments that address both proteins to improve patient outcomes.

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

See full target information HCV subtype 1b NS5B

대체 명칭 보기

Hepacivirin, NS3P, POLG_HCVBK, Serine protease NS3

style

left-column

제품이 사용된 논문 (18)

Recent publications for all applications. Explore the 전체 목록 and refine your search

PloS one 19:e0303265 PubMed38739590

2024

Immortalized hepatocyte-like cells: A competent hepatocyte model for studying clinical HCV isolate infection.

Applications

Unspecified application

Species

Unspecified reactive species

Yongyut Pewkliang,Piyanoot Thongsri,Phichaya Suthivanich,Nipa Thongbaiphet,Jiraporn Keatkla,Ekawat Pasomsub,Usanarat Anurathapan,Suparerk Borwornpinyo,Adisak Wongkajornsilp,Suradej Hongeng,Khanit Sa-Ngiamsuntorn

International journal of molecular sciences 22: PubMed33925399

2021

Pre-Senescence Induction in Hepatoma Cells Favors Hepatitis C Virus Replication and Can Be Used in Exploring Antiviral Potential of Histone Deacetylase Inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Alsu Z Malikova,Anastasia S Shcherbakova,Konstantin A Konduktorov,Anastasia S Zemskaya,Alexandra A Dalina,Vladimir I Popenko,Olga G Leonova,Alexei V Morozov,Nikolay N Kurochkin,Olga A Smirnova,Sergey N Kochetkov,Maxim V Kozlov

Biophysical journal 116:1328-1339 PubMed30879645

2019

A Nondimensional Model Reveals Alterations in Nuclear Mechanics upon Hepatitis C Virus Replication.

Applications

Unspecified application

Species

Unspecified reactive species

Sreenath Balakrishnan,Suma S Mathad,Geetika Sharma,Shilpa R Raju,Uma B Reddy,Saumitra Das,G K Ananthasuresh

Scientific reports 7:11250 PubMed28900156

2017

Dual Roles of Two Isoforms of Autophagy-related Gene ATG10 in HCV-Subgenomic replicon Mediated Autophagy Flux and Innate Immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Qiong Zhao,Zhan-Ying Hu,Jing-Pu Zhang,Jian-Dong Jiang,Yuan-Yuan Ma,Jian-Rui Li,Zong-Gen Peng,Jin-Hua Chen

Journal of virology 90:6022-6035 PubMed27099311

2016

NOD1 Participates in the Innate Immune Response Triggered by Hepatitis C Virus Polymerase.

Applications

Unspecified application

Species

Unspecified reactive species

Serena Vegna,Damien Gregoire,Marie Moreau,Patrice Lassus,David Durantel,Eric Assenat,Urszula Hibner,Yannick Simonin

Antimicrobial agents and chemotherapy 60:3540-50 PubMed27021315

2016

Hepatitis C Virus RNA-Dependent RNA Polymerase Interacts with the Akt/PKB Kinase and Induces Its Subcellular Relocalization.

Applications

Unspecified application

Species

Unspecified reactive species

María Llanos Valero,Rosario Sabariegos,Francisco J Cimas,Celia Perales,Esteban Domingo,Ricardo Sánchez-Prieto,Antonio Mas

Journal of virology 89:11356-71 PubMed26339049

2015

HuR Displaces Polypyrimidine Tract Binding Protein To Facilitate La Binding to the 3' Untranslated Region and Enhances Hepatitis C Virus Replication.

Applications

Species

Human

Shivaprasad Shwetha,Anuj Kumar,Ranajoy Mullick,Deeptha Vasudevan,Nilanjan Mukherjee,Saumitra Das

International journal of molecular medicine 35:791-7 PubMed25572289

2015

A zebrafish model for subgenomic hepatitis C virus replication.

Applications

Unspecified application

Species

Unspecified reactive species

Cun-Bao Ding,Ye Zhao,Jing-Pu Zhang,Zong-Gen Peng,Dan-Qing Song,Jian-Dong Jiang

Antimicrobial agents and chemotherapy 59:85-95 PubMed25313218

2014

The bioactive lipid 4-hydroxyphenyl retinamide inhibits flavivirus replication.

Applications

Unspecified application

Species

Unspecified reactive species

Margot Carocci,Stephen M Hinshaw,Mary A Rodgers,Valerie A Villareal,Dominique J Burri,Rajendra Pilankatta,Natalya P Maharaj,Michaela U Gack,Eric J Stavale,Kelly L Warfield,Priscilla L Yang

PloS one 8:e82527 PubMed24358200

2013

Isolation and characterization of highly replicable hepatitis C virus genotype 1a strain HCV-RMT.

Applications

Species

Unspecified reactive species

Masaaki Arai,Yuko Tokunaga,Asako Takagi,Yoshimi Tobita,Yuichi Hirata,Yuji Ishida,Chise Tateno,Michinori Kohara

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HCV subtype 1b NS5B antibody

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

대체품

Primary Antibodies

보완 제품

Proteins & Peptides

Primary Antibodies

Primary Antibodies

Assay Kits

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (18)

Product promise