AB327389

Anti-HDAC2 antibody [EPR20117-14] - BSA and Azide free (Capture)

RabMAb
Recombinant
KO Validated
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Rabbit Recombinant Monoclonal HDAC2 antibody. Carrier free. Suitable for sELISA and reacts with Human, Mouse, Rat samples.

대체 명칭 보기

Histone deacetylase 2, HD2, Protein deacylase HDAC2, HDAC2

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Sandwich ELISA - Anti-HDAC2 antibody [EPR20117-14] - BSA and Azide free (Capture) (AB327389)

sELISA

Lab

Sandwich ELISA - Anti-HDAC2 antibody [EPR20117-14] - BSA and Azide free (Capture) (AB327389)

Interpolated concentration of native HDAC2 was measured at different sample concentrations. Undiluted samples are as follows : HEK293T cell extract (20 µg/mL) and HeLa cell extract (12.5 µg/mL). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

sELISA

Lab

Sandwich ELISA - Anti-HDAC2 antibody [EPR20117-14] - BSA and Azide free (Capture) (AB327389)

Sandwich ELISA with the capture antibody ab327389 dilution at 2 µg/mL and detector antibody ab327390 dilution at 0.5 µg/mL after biotin conjugation.

sELISA

Lab

Sandwich ELISA - Anti-HDAC2 antibody [EPR20117-14] - BSA and Azide free (Capture) (AB327389)

Interpolated concentrations of native HDAC2 in human control wild type HEK293T (human embryonic kidney epithelial cell) and HDAC2 knockout HEK293T cell based on 20 µg/mL extract loads. The concentrations of HDAC2 were measured in duplicate and interpolated from the HDAC2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HDAC2 concentration was determined to be 6428.2 pg/mL in wild type HEK293T extract (Human wild-type HEK293T cell line ab255449) and undetectable in HDAC2 knockout HEK293T extract (Human HDAC2 knockout HEK293T cell line ab266590).

sELISA

Lab

Sandwich ELISA - Anti-HDAC2 antibody [EPR20117-14] - BSA and Azide free (Capture) (AB327389)

Interpolated concentration of native HDAC2 was measured at different sample concentrations. Undiluted samples are as follows : C6 cell extract (50 µg/mL) and rat brain tissue extract (200 µg/mL). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

sELISA

Lab

Sandwich ELISA - Anti-HDAC2 antibody [EPR20117-14] - BSA and Azide free (Capture) (AB327389)

Interpolated concentration of native HDAC2 was measured at different sample concentrations. Undiluted samples are as follows : NIH/3T3 cell extract (50 µg/mL) and mouse brain tissue extract (50 µg/mL). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20117-14

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody is specific to HDAC2 and shows no cross-reactivity with human HDAC1, or HDAC3. The antibody pair, consisting of ab327389 as the capture and ab327390 as the detector, exhibits no cross-reactivity with human HDAC1, or HDAC3.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "" }, "Mouse": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "" }, "Rat": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "" } } }

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제품 세부 정보

ab327389 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human,Mouse,Rat HDAC2. The recommended pair for sandwich ELISA is:

Capture: ab327389, Human, Mouse, Rat HDAC2 Capture Antibody (unconjugated)

Detector: ab327390, Human, Mouse, Rat HDAC2 Detector Antibody (unconjugated)

The reference range value is 78.1-5000 pg/mL.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Histone deacetylase that catalyzes the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) (PubMed : 28497810). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events (By similarity). Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR (PubMed : 12724404). Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development (By similarity). Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin (PubMed : 16428440, PubMed : 28977666). Component of the SIN3B complex that represses transcription and counteracts the histone acetyltransferase activity of EP300 through the recognition H3K27ac marks by PHF12 and the activity of the histone deacetylase HDAC2 (PubMed : 37137925). Also deacetylates non-histone targets : deacetylates TSHZ3, thereby regulating its transcriptional repressor activity (PubMed : 19343227). May be involved in the transcriptional repression of circadian target genes, such as PER1, mediated by CRY1 through histone deacetylation (By similarity). Involved in MTA1-mediated transcriptional corepression of TFF1 and CDKN1A (PubMed : 21965678). In addition to protein deacetylase activity, also acts as a protein-lysine deacylase by recognizing other acyl groups : catalyzes removal of (2E)-butenoyl (crotonyl), lactoyl (lactyl) and 2-hydroxyisobutanoyl (2-hydroxyisobutyryl) acyl groups from lysine residues, leading to protein decrotonylation, delactylation and de-2-hydroxyisobutyrylation, respectively (PubMed : 28497810, PubMed : 29192674, PubMed : 35044827).

See full target information HDAC2

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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