AB131524

Anti-HDAC4 + 5 + 9 antibody

Name: Anti-HDAC4 + 5 + 9 antibody
Brand: Abcam Limited
SKU: AB131524
Rating: 4 (2 reviews)

(2 리뷰들)

(3 제품이 사용된 논문 )

Rabbit Polyclonal HDAC4 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human HDAC4 aa 200-250.

대체 명칭 보기

KIAA0288, HDAC4, Histone deacetylase 4, HD4, HDAC7, HDAC7B, HDRP, KIAA0744, MITR, HDAC9, Histone deacetylase 9, HD9, Histone deacetylase 7B, Histone deacetylase-related protein, MEF2-interacting transcription repressor MITR, HD7, HD7b, KIAA0600, HDAC5, Histone deacetylase 5, HD5, Antigen NY-CO-9

3 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC4 + 5 + 9 antibody (AB131524)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC4 + 5 + 9 antibody (AB131524)

Immunohistochemical analysis of paraffin embedded Human lung carcinoma tissue labelled with ab131524 at 1/50 dilution (left) or the same antibody preincubated with blocking peptide (right).

Immunocytochemistry/ Immunofluorescence - Anti-HDAC4 + 5 + 9 antibody (AB131524)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-HDAC4 + 5 + 9 antibody (AB131524)

Immunofluorescence analysis of methanol-fixed HeLa cells labelled with ab131524 at 1/100 dilution.

Unknown

Western blot - Anti-HDAC4 + 5 + 9 antibody (AB131524)

All lanes:

Western blot - Anti-HDAC4 + 5 + 9 antibody (ab131524) at 1/500 dilution

Lane 1:

293 cell lysate

Lane 2:

HeLa cell lysate

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human HDAC4 aa 200-250. The exact immunogen used to generate this antibody is proprietary information.

P56524

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/200", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/100 - 1/200", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

HDAC4 HDAC5 and HDAC9 are types of histone deacetylases abbreviated as HDACs. These enzymes remove acetyl groups from histone and non-histone proteins affecting gene expression by changing chromatin structure. HDAC4 has a molecular weight of about 140 kDa. HDAC4 and HDAC5 are highly expressed in the brain heart and skeletal muscle while HDAC9 is more abundant in the heart and skeletal muscle. They act as transcriptional repressors by interacting with various corepressor complexes.

Biological function summary

Histone deacetylases 4 5 and 9 influence gene expression and cellular differentiation. These proteins often form part of larger corepressor complexes such as the N-CoR and SMRT complexes allowing them to modulate transcriptional activity. They play significant roles in muscle differentiation and neural development interacting with transcription factors like MEF2 to regulate various genes involved in these processes.

Pathways

Histone deacetylases like HDAC4 5 and 9 are critical components of signaling pathways related to calcium/calmodulin-dependent calcineurin signal transduction. This pathway influences muscle development and function where they interact with MEF2 family transcription factors to control gene expression. Additionally they are involved in the regulation of the circadian rhythm through their role in the molecular clockwork partnering with proteins like CLOCK and BMAL1.

Alterations in HDAC4 5 and 9 levels or activity have connections to cardiovascular diseases and certain cancers. Dysfunctional HDAC activity can lead to hypertrophy in cardiac cells affecting heart function. Cancer progression often involves aberrant histone deacetylation with HDACs modulating oncogenic transcription factors. These HDACs closely interact with cancer-related proteins like MYC and p53 influencing tumor suppression or progression depending on cellular context.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. Deacetylates HSPA1A and HSPA1B at 'Lys-77' leading to their preferential binding to co-chaperone STUB1 (PubMed : 27708256).

See full target information HDAC4

Additional targets

HDAC9,HDAC5

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell reports 43:114054 PubMed38578832

2024

14-3-3 binding motif phosphorylation disrupts Hdac4-organized condensates to stimulate cardiac reprogramming.

Applications

Unspecified application

Species

Unspecified reactive species

Liu Liu,Ienglam Lei,Shuo Tian,Wenbin Gao,Yijing Guo,Zhaokai Li,Ziad Sabry,Paul Tang,Y Eugene Chen,Zhong Wang

eLife 12: PubMed37902809

2023

The PMA phorbol ester tumor promoter increases canonical Wnt signaling via macropinocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Nydia Tejeda-Munoz,Yagmur Azbazdar,Julia Monka,Grace Binder,Alex Dayrit,Raul Ayala,Neil O'Brien,Edward M De Robertis

ChemMedChem 14:1096-1107 PubMed30921497

2019

Structurally Diverse Histone Deacetylase Photoreactive Probes: Design, Synthesis, and Photolabeling Studies in Live Cells and Tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Shaimaa M Aboukhatwa,Thomas W Hanigan,Taha Y Taha,Jayaprakash Neerasa,Rajeev Ranjan,Eman E El-Bastawissy,Mohamed A Elkersh,Tarek F El-Moselhy,Jonna Frasor,Nadim Mahmud,Alan McLachlan,Pavel A Petukhov

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HDAC4 + 5 + 9 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC4 + 5 + 9 antibody (AB131524)

Immunocytochemistry/ Immunofluorescence - Anti-HDAC4 + 5 + 9 antibody (AB131524)

Western blot - Anti-HDAC4 + 5 + 9 antibody (AB131524)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

Additional targets

제품이 사용된 논문 (3)

Product promise