Anti-HDAC4 (phospho S632) antibody

• WB

Collaborator

Western blot - Anti-HDAC4 (phospho S632) antibody (AB39408)

All lanes:

Western blot - Anti-HDAC4 (phospho S632) antibody (ab39408) at 1/500 dilution

Lane 1:

Lysate from murine heart tissue 1 at 20 µg

Lane 2:

Lysate from murine heart tissue 2 at 20 µg

Secondary

All lanes:

HRp conjugated goat anti-rabbit polyclonal at 1/5000 dilution

Predicted band size: 119 kDa

Observed band size: 130 kDa

true

Exposure time: 1min

Image courtesy of an anonymous Abreview.

• WB

PubMed

Western blot - Anti-HDAC4 (phospho S632) antibody (AB39408)

20 µg of nuclear lysate prepared from mouse liver was diluted in SDS buffer and run on SDS-PAGE gel. Samples were transferred to nitrocellulose overnight, and blocked with 5% BSA for 8 hours. They were then incubated overnight with primary antibodies, which included an anti-HDAC1, 1/1000 and ab39408, 1/1000. After washing, membranes were incubated with horseradish peroxidase-coupled rabbit or mouse secondary antibodies (1/5000 to 1/10,000) in 5% milk for 1 hour and developed with a Super Signal West Pico chemiluminescent kit.

All lanes:

Western blot - Anti-HDAC4 (phospho S632) antibody (ab39408)

Predicted band size: 119 kDa

false

Image from Evankovich J et al, J Biol Chem. 2010 Dec 17;285(51):39888-97. Epub 2010 Oct 11, Fig 4.

• WB

Unknown

Western blot - Anti-HDAC4 (phospho S632) antibody (AB39408)

All lanes:

Western blot - Anti-HDAC4 (phospho S632) antibody (ab39408) at 1/500 dilution

Lane 1:

Extract from Jurkat cells (5-30ug of total protein)

Lane 2:

Extract from Jurkat cells treated with Calyculin A (5-30ug of total protein)

Predicted band size: 119 kDa

false