AB248571

Anti-HDLBP antibody [EPR5268] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal HDLBP antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

대체 명칭 보기

HBP, VGL, HDLBP, Vigilin, High density lipoprotein-binding protein, HDL-binding protein

2 이미지

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Western blot - Anti-HDLBP antibody [EPR5268] - BSA and Azide free (AB248571)

This data was developed using ab133594, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-HDLBP antibody [EPR5268] (<a href='/ko/products/primary-antibodies/hdlbp-antibody-epr5268-ab133594'>ab133594</a>) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Lane 5:

RAW 264.7 cell lysate at 10 µg

Lane 6:

PC12 cell lysate at 10 µg

Lane 7:

NIH 3T3 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

Predicted band size: 141 kDa

Observed band size: 155 kDa

false

OI-RD Scanning - Anti-HDLBP antibody [EPR5268] - BSA and Azide free (AB248571)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-HDLBP antibody [EPR5268] - BSA and Azide free (AB248571)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5268

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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제품 세부 정보

ab248571 is the carrier-free version of ab133594.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The HDLBP also known as Vigilin is a protein that interacts with high-density lipoproteins (HDL). It has a molecular mass of approximately 116 kDa. HDLBP plays an important role in binding RNA and is largely expressed in the liver and adipose tissues. Through its RNA-binding capacity HDLBP regulates the stability and translation of specific mRNAs impacting various cellular processes.

Biological function summary

Without HDLBP's regulatory functions in RNA metabolism cells struggle to maintain proper gene expression. This protein is not known to operate as part of a multi-protein complex but acts independently to influence RNA molecule interactions. HDLBP regulates lipid metabolism by facilitating the transport and stabilization of RNA species that encode enzymes important for lipid synthesis and uptake.

Pathways

The RNA-binding functions of HDLBP integrate into lipid metabolism and transport pathways. In the lipid metabolic pathway HDLBP interacts with other proteins such as apolipoproteins to manage lipid homeostasis. In addition it plays a part in the RNA processing and transport pathway linking its activity to proteins involved in RNA export and translation control.

Without balanced HDLBP activity individuals show an increased risk of developing atherosclerosis and metabolic syndrome. The improper regulation of HDL and lipid metabolism contributes to these conditions. HDLBP's role overlaps with the functions of apolipoprotein A-I in these disorders as both participate in managing HDL-cholesterol levels and lipid homeostasis. Understanding HDLBP's mechanisms can help target therapeutic strategies for cardiovascular diseases.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Appears to play a role in cell sterol metabolism. It may function to protect cells from over-accumulation of cholesterol.

See full target information HDLBP

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HDLBP antibody [EPR5268] - BSA and Azide free

Western blot - Anti-HDLBP antibody [EPR5268] - BSA and Azide free (AB248571)

OI-RD Scanning - Anti-HDLBP antibody [EPR5268] - BSA and Azide free (AB248571)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

Product promise