AB241610

Anti-HEATR1 antibody

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(3 제품이 사용된 논문 )

Rabbit Polyclonal HEATR1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human HEATR1 aa 2050 to C-terminus.

대체 명칭 보기

BAP28, UTP10, HEATR1, HEAT repeat-containing protein 1, Protein BAP28, U3 small nucleolar RNA-associated protein 10 homolog

2 이미지

Supplier Data

Immunoprecipitation - Anti-HEATR1 antibody (AB241610)

HEATR1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241610 at 6 μg/reaction. Western blot was performed from the immunoprecipitate using ab241610 at 0.4 μg/ml.

Lane 1 : ab241610 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with exposure time of 3 seconds.

Prepared using NETN lysis buffer.

All lanes:

Immunoprecipitation - Anti-HEATR1 antibody (ab241610)

Predicted band size: 242 kDa

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Supplier Data

Western blot - Anti-HEATR1 antibody (AB241610)

Prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-HEATR1 antibody (ab241610) at 0.04 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate at 50 µg

Predicted band size: 242 kDa

true

Exposure time: 30s

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human HEATR1 aa 2050 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9H583

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/25000", "WB-species-notes": "<p></p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

HEATR1 also known as HEAT repeat-containing protein 1 is a cytoplasmic protein with a molecular mass of approximately 166 kDa. The protein features several HEAT repeats which are tandemly arranged alpha-helical motifs. HEATR1 expresses in various tissues with high levels detected in the testes and lower levels in brain and lungs. The protein plays a role in cellular processes like protein trafficking and folding contributing to ribosome biogenesis and maintenance of cell cycle progression.

Biological function summary

HEATR1 influences pathways involved in the assembly and maturation of the ribosomal machinery. It forms part of the ribosome assembly complex associating with other ribosome biogenesis factors to facilitate ribosome production. The involvement of HEATR1 in the assembly complex helps ensure the ribosomes are correctly formed and functional impacting protein synthesis efficiency.

Pathways

HEATR1 interacts with key regulators in the ribosome biogenesis pathway and is linked to cell cycle regulation. It connects to proteins such as NOP14 and WDR25 within these pathways highlighting its importance in ribosome assembly and cellular division. The accurate functioning of these pathways is critical for cell growth and proliferation affecting overall cellular health and metabolism.

HEATR1 may be related to ribosomopathies and certain cancers where defects in ribosome biogenesis or aberrant protein synthesis play a role. The protein’s interactions with NOP14 suggest potential connections with disorders in ribosome production which may lead to proliferation issues observed in malignancies. Understanding the role of HEATR1 can offer insights into the molecular mechanisms behind these conditions aiding in the development of new therapeutic strategies.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Ribosome biogenesis factor; required for recruitment of Myc to nucleoli (PubMed : 38225354). Involved in nucleolar processing of pre-18S ribosomal RNA. Required for optimal pre-ribosomal RNA transcription by RNA polymerase I (PubMed : 17699751). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed : 34516797). Involved in neuronal-lineage cell proliferation (PubMed : 38225354).

See full target information HEATR1

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature 614:160-167 PubMed36697828

2023

Visualization of translation and protein biogenesis at the ER membrane.

Applications

Unspecified application

Species

Unspecified reactive species

Max Gemmer,Marten L Chaillet,Joyce van Loenhout,Rodrigo Cuevas Arenas,Dimitrios Vismpas,Mariska Gröllers-Mulderij,Fujiet A Koh,Pascal Albanese,Richard A Scheltema,Stuart C Howes,Abhay Kotecha,Juliette Fedry,Friedrich Förster

Biochemical and biophysical research communications 557:294-301 PubMed33894417

2021

HEATR1, a novel interactor of Pontin/Reptin, stabilizes Pontin/Reptin and promotes cell proliferation of oral squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Akihiko Nakamura,Yoshito Kakihara,Akinori Funayama,Kenta Haga,Toshihiko Mikami,Daiki Kobayashi,Yutaka Yoshida,Kenji Izumi,Tadaharu Kobayashi,Makio Saeki

Journal of oncology 2020:3181596 PubMed32565799

2020

HEATR1 Deficiency Promotes Chemoresistance via Upregulating ZNF185 and Downregulating SMAD4 in Pancreatic Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Fang,Xu Han,Jianang Li,Tiantao Kuang,Wenhui Lou

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HEATR1 antibody

Immunoprecipitation - Anti-HEATR1 antibody (AB241610)

Western blot - Anti-HEATR1 antibody (AB241610)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (3)

Product promise