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AB241413

Anti-HECTD3 antibody

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Rabbit Polyclonal HECTD3 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human E3 ubiquitin-protein ligase HECTD3 aa 800-900.

대체 명칭 보기

E3 ubiquitin-protein ligase HECTD3, HECT domain-containing protein 3, HECT-type E3 ubiquitin transferase HECTD3, HECTD3

2 이미지
Immunoprecipitation - Anti-HECTD3 antibody (AB241413)
  • IP

Supplier Data

Immunoprecipitation - Anti-HECTD3 antibody (AB241413)

HECTD3 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241413 at 6 μg/reaction. Western blot was performed from the immunoprecipitate using ab241413 at 1 μg/ml.

Lane 1 : ab241413 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with exposure time of 3 minutes.

Lysates prepared using NETN lysis buffer.

All lanes:

Immunoprecipitation - Anti-HECTD3 antibody (ab241413)

Predicted band size: 97 kDa

false

Western blot - Anti-HECTD3 antibody (AB241413)
  • WB

Supplier Data

Western blot - Anti-HECTD3 antibody (AB241413)

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-HECTD3 antibody (ab241413) at 1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 97 kDa

true

Exposure time: 30s

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human E3 ubiquitin-protein ligase HECTD3 aa 800-900. The exact immunogen used to generate this antibody is proprietary information.

Q5T447

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보완 제품

Primary Antibodies

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Primary Antibodies

AB214671

Anti-NAGLU/NAG antibody [EPR20708]

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Primary Antibodies

AB133502

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  • 0
  • 0
Primary Antibodies

AB228729

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  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2500", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2500", "WB-species-notes": "<p></p>" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
Purification 관련 사항
ab241413 was affinity purified using an epitope specific to HECTD3 immobilized on solid support.
보관 버퍼
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

HECTD3 also known as hectd3 ubiquitin protein ligase acts as an E3 ubiquitin ligase. It plays an important role in tagging proteins for degradation through the ubiquitin-proteasome system. The molecular weight of HECTD3 is approximately 110 kDa. HECTD3 is mainly expressed in the brain and various immune cells indicating its involvement in neurological and immune functions.
Biological function summary

The enzyme facilitates the regulation of proteins by adding ubiquitin molecules which marks them for degradation or signaling. This function helps control the levels of target proteins and maintain cellular homeostasis. HECTD3 often forms part of a protein complex enabling its ligase activity to be moderated by interacting proteins.

Pathways

The enzyme integrates into the ubiquitin-proteasome pathway and the NF-kB signaling pathway. In the ubiquitin-proteasome system HECTD3 collaborates with other E2 and E1 enzymes to achieve efficient ubiquitination. It interacts directly with IκB proteins in the NF-kB signaling pathway impacting inflammatory and immune responses by controlling the stability and activity of these proteins.

HECTD3's malfunctions correlate to various cancers and immune disorders. For example its dysregulation affects certain types of leukemia where it modifies the degradation process of tumor suppressor proteins. Moreover HECTD3 interacts with proteins such as TP53 which also plays a significant role in the development of cancers. Changes in HECTD3 expression in immune cells influence immune disorders connecting it to autoimmune diseases where immune regulation becomes disrupted.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

E3 ubiquitin ligases accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates ubiquitination of TRIOBP and its subsequent proteasomal degradation, thus facilitating cell cycle progression by regulating the turn-over of TRIOBP. Mediates also ubiquitination of STX8 (By similarity).
See full target information E3 ubiquitin-protein ligase HECTD3
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Communications biology 5:564 PubMed35681048

2022

Stabilization of CDK6 by ribosomal protein uS7, a target protein of the natural product fucoxanthinol.

Applications

Unspecified application

Species

Unspecified reactive species

Yosuke Iizumi,Yoshihiro Sowa,Wakana Goi,Yuichi Aono,Motoki Watanabe,Yoichi Kurumida,Tomoshi Kameda,Kenichi Akaji,Masatoshi Kitagawa,Toshiyuki Sakai
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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