AB243073

Anti-HEL antibody [5E8]

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Mouse Monoclonal HEL antibody. Suitable for IHC-P, WB, Flow Cyt and reacts with Rat, Chemical samples. Cited in 1 publication. Immunogen corresponding to Chemical / Small Molecule corresponding to HEL.

대체 명칭 보기

Hexanoyl Lys

4 이미지

Flow Cytometry - Anti-HEL antibody [5E8] (AB243073)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-HEL antibody [5E8] (AB243073)

Flow cytometric analysis of 90% methanol-fixed SH-SY5Y (human neuroblastoma cell line from bone marrow) cells labeling HEL with ab243073 at 1/50 dilution for 30 mins on ice (solid line) compared with an Isotype control IgG (dashed line). A Goat Anti-Mouse : PE at 1/100 dilution was used as the secondary antibody (20 mins at RT).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEL antibody [5E8] (AB243073)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEL antibody [5E8] (AB243073)

Immunohistochemistry analysis of Rat heart tissue using Anti-HEL antibody [5E8] (ab243073) Fixation : Formalin fixed, paraffin embedded. Primary Antibody : ab243073 at 1 : 26 for 2 hour at RT. Secondary Antibody : Goat Anti-Mouse IgG : Alexa Fluor 489. Counterstain : DAPI (blue) nuclear stain. Magnification : 63X. (A) DAPI (blue) nuclear stain. (B) Actin (C) ab243073 (D) Merged.

Supplier Data

Western blot - Anti-HEL antibody [5E8] (AB243073)

Primary incubation for 2 hrs at RT.

Secondary incubation for 1 hr at RT.

Block : 5% Skimmed milk in TBST.

All lanes:

Western blot - Anti-HEL antibody [5E8] (ab243073) at 1/1000 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 12 µg

Lane 2:

HeLa (treated with hydrogen peroxide) cell lysate at 12 µg

Secondary

All lanes:

Goat Anti-Mouse IgG: HRP at 1/2000 dilution

true

Exposure time: 5min

Supplier Data

Western blot - Anti-HEL antibody [5E8] (AB243073)

Primary incubation for 2 hours at room temperature.

Secondary incubation for 1 hour at room temperature.

Block : 5% Skimmed milk in TBST.

All lanes:

Western blot - Anti-HEL antibody [5E8] (ab243073) at 1/1000 dilution

Lane 1:

Hexanoyl Lysine-BSA at 0.5 µg

Lane 2:

BSA at 0.5 µg

Secondary

All lanes:

Goat Anti-Mouse IgG: HRP at 1/2000 dilution

true

Exposure time: 5min

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

5E8

Isotype

IgG1

Carrier free

Applications

Flow Cyt, IHC-P, WB

applications

Specificity

Specific for Hexanoyl-Lysine adduct (HEL) modified peptides & proteins. Does not detect free HEL. Does not X-react with Acrolein, Crotonaldehyde, 4-HHE, 4-HNE, MDA, or MGO modified proteins.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/26", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Chemical": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/50", "FlowCyt-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

Purification 관련 사항

Purified from TCS.

보관 버퍼

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

HEL or hen egg white lysozyme is an enzyme that cleaves the glycosidic linkages in the polysaccharides of bacterial cell walls. This enzyme has a known mass of approximately 14 kDa. You can find HEL expressed in various tissues primarily within the egg whites of birds. There are several forms of HEL including HEL full form which refers to its complete structure used in studies assessing enzyme-substrate interactions. Researchers often use anti-HEL antibodies to track HEL activity or in assays like 30 minutes of HEL which can indicate enzyme activity within a specific time frame.

Biological function summary

HEL plays an important role in innate immune defense by degrading peptidoglycan components of bacterial cell membranes. It does not function within a larger protein complex but possesses its antimicrobial activity independently. Typically found in saliva tears and mucus HEL contributes to the first line of defense against bacterial invasion. HEL's antimicrobial property also positions it as an influential protein for studies concerning bacterial resistance and immune response in higher organisms.

Pathways

HEL participates mainly in the defense mechanism pathways including the peptidoglycan degradation pathway. This pathway which involves breaking down bacterial cell walls links HEL intimately with proteins like N-acetylmuramyl-L-alanine amidase. While HEL acts alone in its enzymatic activity it complements actions of other antimicrobial proteins to form a robust defense network. Connections between HEL's role and other pathways may provide insights into broader immune responses but these remain a focused area for study.

HEL is particularly connected to infectious diseases linked with bacterial pathogens such as bacterial conjunctivitis. In such cases HEL activity correlates with proteins involved in local immune responses including lysozyme C. Though HEL's direct connection to non-infectious disorders remains less explored its application in disease models serves as a stepping stone for understanding other disorders. Paying attention to HEL and associated pathways through anti-HEL and anti-lysozyme antibodies can lend more understanding in pathological studies focused on bacterial infections.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

See full target information HEL

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

International journal of molecular sciences 25: PubMed39769037

2025

L. Leaf Extract Dose-Dependently Modulates Oxidative Stress in the Kidney and Exerts Anti-Fibrotic and Anti-Inflammatory Properties by the Molecular Mechanisms Independent of NRF-2 Signalization Mirroring the Effects of Losartan in SHR.

Applications

Unspecified application

Species

Unspecified reactive species

Una-Jovana Vajic,Nevena Mihailovic-Stanojevic,Danijela Karanovic,Maja Zivotic,Milan Ivanov,Djurdjica Jovovic,Jelica Grujic-Milanovic,Zoran Miloradovic

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HEL antibody [5E8]

Flow Cytometry - Anti-HEL antibody [5E8] (AB243073)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEL antibody [5E8] (AB243073)

Western blot - Anti-HEL antibody [5E8] (AB243073)

Western blot - Anti-HEL antibody [5E8] (AB243073)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Applications

Specificity

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise