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AB210851

Anti-Helicobacter pylori antibody [EPR10353] - BSA and Azide free

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Rabbit Recombinant Monoclonal Helicobacter pylori antibody. Carrier free. Suitable for IHC-P and reacts with Helicobacter pylori samples. Cited in 1 publication.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Helicobacter pylori antibody [EPR10353] - BSA and Azide free (AB210851)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Helicobacter pylori antibody [EPR10353] - BSA and Azide free (AB210851)

This data was developed using ab172611, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human HP(+)gastritis tissue sections labeling Helicobacter pylori with purified ab172611 at 1/13000 dilution (0.03 μg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10353

Isotype

IgG

Carrier free

Yes

Reacts with

Helicobacter pylori

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Assay Kits

AB150673

H. Pylori Stain Kit (Microorganism Stain)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - H. Pylori Stain Kit (Microorganism Stain) (AB150673)

  • 0
  • 0
Sample Prep & Detection Kits

AB102890

HRP Conjugation Kit - Lightning-Link®

Conjugation - HRP Conjugation Kit - Lightning-Link® (AB102890)

  • 4
  • 3
Sample Prep & Detection Kits

AB269903

Gold-Maleimide Conjugation Kit (10nm, 20 OD)

Lateral flow assay - Gold-Maleimide Conjugation Kit (10nm, 20 OD) (AB269903)

  • 0
  • 0
Primary Antibodies

AB219650

Anti-PRAME antibody [EPR20330]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRAME antibody [EPR20330] (AB219650)

  • 0
  • 0
Primary Antibodies

AB172611

Anti-Helicobacter pylori antibody [EPR10353]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Helicobacter pylori antibody [EPR10353] (AB172611)

  • 0
  • 0
Primary Antibodies

AB51608

Anti-HIF-1 alpha antibody [EP1215Y]

20ul selling size|KO Validated|RabMAb|Advanced Validation|Recombinant

Western blot - Anti-HIF-1 alpha antibody [EP1215Y] (AB51608)

  • 4
  • 15
Primary Antibodies

AB20459

Anti-Helicobacter pylori antibody

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Helicobacter pylori": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
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제품 세부 정보

ab210851 is the carrier-free version of ab172611.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Do Not Freeze
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Helicobacter pylori often called H. pylori is a gram-negative spiral-shaped bacterium with a mass of about 3.39 megadaltons. This organism colonizes the human gastric epithelium where it survives in the acidic environment of the stomach. The bacterium expresses urease which breaks down urea to ammonia and carbon dioxide helping neutralize gastric acid. It attaches to the gastric mucosa using flagella for motility and adheres to host cells via adhesins. Researchers utilize specific staining methods such as the Helicobacter pylori stain and tools like anti-Helicobacter antibodies to detect and study this pathogen.
Biological function summary

Helicobacter pylori plays a significant role in gastric health by disrupting mucosal integrity. It engages in the secretion of virulence factors like CagA and VacA leading to changes in cellular signaling and inflammation. H. pylori does not operate as part of a large complex but its interactions with the gastric mucosa have substantial effects. Detection and diagnosis often employ tools like H. pylori IHC and H. pylori antibody kits which target the bacterium's proteins to confirm infection presence.

Pathways

H. pylori infection activates signaling routes including the NF-κB pathway and the MAPK pathway in gastric epithelial cells. These pathways contribute to inflammatory responses and immune cell activation leading to mucosal damage. Within these pathways proteins like tumor necrosis factor-alpha (TNF-α) and interleukin-8 (IL-8) play roles in mediating inflammation initiated by the bacterial colonization of the gastric lining.

Chronic H. pylori infection has strong associations with gastric ulcers and gastric cancer. The bacterium promotes persistent inflammation and cellular damage that can progress to malignancy. H. pylori-related disorders are linked with proteins such as CagA a known oncogenic factor that interferes with host cell signaling and promotes carcinogenesis. Detection and specific targeting by Helicobacter pylori antibodies play important roles in managing and studying these conditions.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

See full target information Helicobacter pylori

대체 명칭 보기

H. pylori
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Blood science (Baltimore, Md.) 7:e00236 PubMed40416726

2025

Super-resolution imaging informed scRNA sequencing analysis reveals the critical role of GDF15 in rejuvenating aged hematopoietic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongyu Shi,Xuefei Zhang,Huihui Yang,Xiaolu Zheng,Mengxiao Niu,Yongjian Zhang,Pengfei Yuan,Wensheng Wei,Gang Huang,Riguo Fang,Liangyi Chen
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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