Anti-Hepatitis B Virus Core Antigen antibody [EPR29890-38]

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepatitis B Virus Core Antigen antibody [EPR29890-38] (AB323646)

Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with Hepatitis B Virus Core Antigen expression vector containing a myc-His-tag;; (B) HEK-293T transfected with empty vector containing a myc-His-tag;. tissue labeling Hepatitis B Virus Core Antigen with ab323646 at 1/1000 (0.488 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in (A) HEK-293T (human embryonic kidney epithelial cell) transfected with Hepatitis B Virus Core Antigen expression vector containing a myc-His-tag, and no signal in (B) HEK-293T transfected with empty vector containing a myc-His-tag.
The section was incubated with ab323646 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Hepatitis B Virus Core Antigen antibody [EPR29890-38] (AB323646)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Hepatitis B Virus Core Antigen with ab323646 at 1/1000 (0.488 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2ug/ml) dilution (Green).

Confocal image showing cytoplasmic and nuclear staining in 293T cells transfected with a Virus wild-type Hepatitis B Virus Core Antigen expression vector containing a Myc tag (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 (5ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2ug/ml) dilution.

• I-ELISA

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Indirect ELISA - Anti-Hepatitis B Virus Core Antigen antibody [EPR29890-38] (AB323646)

Indirect ELISA analysis of ab323646 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution dilution.

Antigen : HBV : Capsid protein.

Antigen concentration : 1000 ng/ml

• WB

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Western blot - Anti-Hepatitis B Virus Core Antigen antibody [EPR29890-38] (AB323646)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-Hepatitis B Virus Core Antigen antibody [EPR29890-38] (ab323646) at 1/1000 dilution

Lane 1:

293T cells (human embryonic kidney epithelial cell) transfected with an empty vector containing a myc-His-tag®, whole cell lysate at 10 µg

Lane 2:

293T cells transfected with a Virus Hepatitis B Virus Core Antigen (genotype A2) vector containing a myc-His-tag®, whole cell lysate at 10 µg

Lane 3:

293T cells transfected with a Virus HBEAG_HBVA7 (genotype A7) vector containing a myc-His-tag®, whole cell lysate at 10 µg

Lane 4:

293T cells transfected with a Virus CAPSD_HBVB1 (genotype B1) vector containing a myc-His-tag®, whole cell lysate at 10 µg

Lane 5:

293T cells transfected with a Virus CAPSD_HBVC5 (genotype C5) vector containing a myc-His-tag®, whole cell lysate at 10 µg

Lane 6:

293T cells transfected with a Virus CAPSD_HBVE1 (genotype E1) vector containing a myc-His-tag®, whole cell lysate at 10 µg

Lanes 7 - 8:

293T cells transfected with a Virus Hepatitis B Virus Core Antigen (genotype D) vector containing a myc-His-tag®, whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 22 kDa,36 kDa

false

Exposure time: 10s

• WB

Supplier Data

Western blot - Anti-Hepatitis B Virus Core Antigen antibody [EPR29890-38] (AB323646)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-Hepatitis B Virus Core Antigen antibody [EPR29890-38] (ab323646) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a myc-His-tag®, whole cell lysate at 4 µg

Lane 2:

293T cells transfected with a Virus wild-type Hepatitis B Virus Core Antigen expression vector containing a myc-His-tag®, whole cell lysate at 4 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 22 kDa,36 kDa

false

Exposure time: 10s