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AB319999

Anti-Hepatitis B Virus Surface Antigen (ayw and ayr) antibody [2D11] - BSA and Azide free

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Mouse Recombinant Monoclonal HBSAG antibody. Carrier free. Suitable for WB, I-ELISA and reacts with Transfected cell lysate - Hepatitis B virus, Recombinant fragment - Hepatitis B virus samples.

대체 명칭 보기

HBsAg

2 이미지
Indirect ELISA - Anti-Hepatitis B Virus Surface Antigen (ayw and ayr) antibody [2D11] - BSA and Azide free (AB319999)
  • I-ELISA

Supplier Data

Indirect ELISA - Anti-Hepatitis B Virus Surface Antigen (ayw and ayr) antibody [2D11] - BSA and Azide free (AB319999)

This data was developed using ab319998, the same antibody clone in a different buffer formulation.

Indirect ELISA analysis of ab319998 at 2000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) at 1 : 1000 dilution.

Antigen : HBsAg ayw Subtype, HBsAg adw Subtype.

Antigen concentration : 5000 ng/ml

Western blot - Anti-Hepatitis B Virus Surface Antigen (ayw and ayr) antibody [2D11] - BSA and Azide free (AB319999)
  • WB

Supplier Data

Western blot - Anti-Hepatitis B Virus Surface Antigen (ayw and ayr) antibody [2D11] - BSA and Azide free (AB319999)

This data was developed using ab319998, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-Hepatitis B Virus Surface Antigen (ayw and ayr) antibody [2D11] (<a href='/ko/products/primary-antibodies/hepatitis-b-virus-surface-antigen-ayw-and-ayr-antibody-2d11-ab319998'>ab319998</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a myc-his tag, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a Hepatitis B virus genotype D subtype ayw (isolate France/Tiollais/1979) expression vector containing a myc-his-tag, whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a Hepatitis B virus genotype A2 subtype adw2 (strain Rutter 1979) (HBV-A) expression vector containing a myc-his-tag, whole cell lysate at 20 µg

Lane 4:

293T cells transfected with a Hepatitis B virus genotype C subtype ayr (isolate Human/Japan/Okamoto) (HBV-C) expression vector containing a myc-his-tag, whole cell lysate at 20 µg

Lane 5:

293T cells transfected with a Hepatitis B virus genotype C subtype adr (isolate Japan/Nishioka/1983) (HBV-C) expression vector containing a myc-his-tag, whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 40 kDa,36 kDa

false

Exposure time: 180s

관련 conjugated 항체와 다양한 조성의 항체 (1)

  • Unconjugated

    Anti-Hepatitis B Virus Surface Antigen (ayw and ayr) antibody [2D11]

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

2D11

Isotype

IgG1

Carrier free

Yes

Reacts with

Hepatitis B virus

Applications

WB, I-ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

<p>This antibody recognizes both Hepatitis B Virus Surface Antigen from strains ayw and ayr, but do not cross-react with stains adw2 and adr.</p>

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보완 제품

Primary Antibodies

AB309352

Anti-Hepatitis B virus Protein X antibody [EPR27041-49]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepatitis B virus Protein X antibody [EPR27041-49] (AB309352)

  • 5
  • 1
Primary Antibodies

AB8638

Anti-Hepatitis B Virus Core Antigen antibody [14E11]

  • 0
  • 0
Primary Antibodies

AB283946

Anti-SARS-CoV-2 Spike Glycoprotein S1 (RBD) antibody [EPR24852-174]

20ul selling size|RabMAb|Recombinant

Indirect ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 (RBD) antibody [EPR24852-174] (AB283946)

  • 0
  • 1
Primary Antibodies

AB8637

Anti-Hepatitis B Virus Core Antigen antibody [C1]

Western blot - Anti-Hepatitis B Virus Core Antigen antibody [C1] (AB8637)

  • 4
  • 1
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Hepatitis B virus": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "predicted", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Recombinant fragment - Hepatitis B virus": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p></p>" }, "Transfected cell lysate - Hepatitis B virus": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" } } }
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제품 세부 정보

ab319999 is the carrier-free version of ab319998.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

See full target information Hepatitis B Virus Surface Antigen (ayw and ayr)
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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