Anti-Hepatitis B Virus Surface Antigen (ayw) antibody [1C10]

• I-ELISA

Supplier Data

Indirect ELISA - Anti-Hepatitis B Virus Surface Antigen (ayw) antibody [1C10] (AB322870)

Indirect ELISA analysis of ab322870 at 2000 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) at 1 : 1000 dilution.

Antigen : Hepatitis B virus genotype D subtype ayw (isolate France/Tiollais/1979).

Antigen concentration : 1000-0 ug/ml

• WB

Supplier Data

Western blot - Anti-Hepatitis B Virus Surface Antigen (ayw) antibody [1C10] (AB322870)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-Hepatitis B Virus Surface Antigen (ayw) antibody [1C10] (ab322870) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a myc-his tag, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a Hepatitis B virus genotype D subtype ayw (isolate France/Tiollais/1979) expression vector containing a myc-his-tag, whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a Hepatitis B virus genotype A2 subtype adw2 (strain Rutter 1979) (HBV-A) expression vector containing a myc-his-tag, whole cell lysate at 20 µg

Lane 4:

293T cells transfected with a Hepatitis B virus genotype C subtype ayr (isolate Human/Japan/Okamoto/-) (HBV-C) expression vector containing a myc-his-tag, whole cell lysate at 20 µg

Lane 5:

293T cells transfected with a Hepatitis B virus genotype C subtype adr (isolate Japan/Nishioka/1983) (HBV-C) expression vector containing a myc-his-tag, whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 40 kDa,36 kDa

false

Exposure time: 180s