AB13833

Anti-Hepatitis C Virus NS5a antibody [H26]

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(11 제품이 사용된 논문 )

Mouse Monoclonal Hepatitis C Virus NS5a antibody. Suitable for ELISA, WB, ICC/IF and reacts with Hepatitis C virus samples. Cited in 11 publications.

대체 명칭 보기

HCV, HCV Non-structural protein 5A, NS5A, Non-structural protein 5A, p56

1 이미지

PubMed

Western blot - Anti-Hepatitis C Virus NS5a antibody [H26] (AB13833)

Representative western blot detecting Hepatitis C Virus NS5a with ab13833.

Amounts of Hepatitis C Virus NS5a were compared to Core after spread of infection to >80% of Huh7.5 cells transfected with H77C(1a) and P346A/P351A/P354A, delta414-428 or "delta414-428+20aa" mutants.

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Western blot - Anti-Hepatitis C Virus NS5a antibody [H26] (ab13833)

false

Image from Scheel TK et al., PLoS Pathog. 2012 May;8(5):e1002696. Epub 2012 May 24.; Fig 6.; doi:10.1371/journal.ppat.1002696; May 24, 2012, PLoS Pathog 8(5): e1002696.

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

H26

Isotype

IgG1

Carrier free

Reacts with

Hepatitis C virus

Applications

ICC/IF, WB, ELISA

applications

Specificity

Hepatitis C Virus antigen NS5a. This antibody works with the HCV genotypes 1a, 1b, 3 and 4, but not with genotype 2a.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Hepatitis C virus": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

보관 버퍼

pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The Hepatitis C Virus NS5a (HCV NS5a) protein often simply referred to as NS5a protein is a non-structural protein important in the hepatitis C virus lifecycle. This phosphoprotein weighs approximately 56 kilodaltons and shows its expression primarily in hepatocytes cells in the liver. Researchers target NS5a as a pivotal modulator in the replication of HCV RNA and its role has made it a main target in antiviral drug development. Renowned for participating in RNA binding and interaction with other viral and host proteins NS5a supports the virus's ability to produce infectious particles making it a focus of antiviral research.

Biological function summary

NS5a plays a multifactorial role acting as part of a larger replication complex involved in HCV replication. It interacts with cellular membranes creating a replication platform for the viral RNA synthesis. Beyond its mechanical functions NS5a influences cellular pathways pivotal in the virus's persistence by modulating host cell signaling lipid metabolism and the immune response. There is active research into how NS5a manipulates host cell responses to establish chronic infection a characteristic aspect of HCV.

Pathways

NS5a significantly influences viral replication and assembly pathways. It interacts with host proteins such as cyclophilin A which regulates replication and lipid droplets important for virus assembly. The protein is integral to the PI3K-Akt pathway which NS5a manipulates to support enhanced viral RNA replication. Through its interactions NS5a links with other HCV proteins like NS3 and NS4a modulating both viral and host processes to facilitate hepatitis C virus survival and replication.

NS5a holds a direct relationship with chronic Hepatitis C infection leading to liver cirrhosis and hepatocellular carcinoma (HCC). Its ability to evade the host immune response and promote viral persistence makes it pivotal in disease progression. In the context of HCC NS5a influences the expression and activity of cellular proteins like p53 critical in cell cycle regulation. Research targets NS5a in therapeutic strategies to halt disease progression and improve patient outcomes in HCV-related liver disease.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

See full target information Hepatitis C Virus NS5a

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제품이 사용된 논문 (11)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Proceedings of the National Academy of Sciences of the United States of America 118: PubMed33649237

2021

N6-methyladenosine modification of HCV RNA genome regulates cap-independent IRES-mediated translation via YTHDC2 recognition.

Applications

Unspecified application

Species

Unspecified reactive species

Geon-Woo Kim,Aleem Siddiqui

Journal of visualized experiments : JoVE : PubMed33191939

2020

Single-cell Multiplexed Fluorescence Imaging to Visualize Viral Nucleic Acids and Proteins and Monitor HIV, HTLV, HBV, HCV, Zika Virus, and Influenza Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Raven Shah,Shuiyun Lan,Maritza N Puray-Chavez,Dandan Liu,Philip R Tedbury,Stefan G Sarafianos

PloS one 15:e0239153 PubMed32941510

2020

Reticulon-3 modulates the incorporation of replication competent hepatitis C virus molecules for release inside infectious exosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Jingjing Li,Ebtisam Abosmaha,Carla S Coffin,Patrick Labonté,Terence Ndonyi Bukong

Scientific reports 10:15290 PubMed32943718

2020

Hepatitis C virus enhances Rubicon expression, leading to autophagy inhibition and intracellular innate immune activation.

Applications

Unspecified application

Species

Unspecified reactive species

Yuto Shiode,Hayato Hikita,Satoshi Tanaka,Kumiko Shirai,Akira Doi,Sadatsugu Sakane,Yugo Kai,Tasuku Nakabori,Ryoko Yamada,Takahiro Kodama,Ryohei Narumi,Ryotaro Sakamori,Hidetoshi Eguchi,Takeshi Tomonaga,Tomohide Tatsumi,Tetsuo Takehara

Cell death & disease 11:200 PubMed32205851

2020

IL28A protein homotetramer structure is required for autolysosomal degradation of HCV-NS5A in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan-Yuan Ma,Jian-Rui Li,Zong-Gen Peng,Jing-Pu Zhang

The Journal of biological chemistry 294:7388-7402 PubMed30862675

2019

Phosphorylated tyrosine 93 of hepatitis C virus nonstructural protein 5A is essential for interaction with host c-Src and efficient viral replication.

Applications

Unspecified application

Species

Unspecified reactive species

Stefan Klinker,Sabine Stindt,Lothar Gremer,Johannes G Bode,Christoph G W Gertzen,Holger Gohlke,Oliver H Weiergräber,Silke Hoffmann,Dieter Willbold

International journal of molecular medicine 42:957-965 PubMed29786754

2018

Regulation of interferon signaling and HCV‑RNA replication by extracellular matrix.

Applications

Unspecified application

Species

Unspecified reactive species

Takuya Kuwashiro,Shinji Iwane,Xia Jinghe,Sachiko Matsuhashi,Yuichiro Eguchi,Keizo Anzai,Kazuma Fujimoto,Toshihiko Mizuta,Naoya Sakamoto,Masanori Ikeda,Nobuyuki Kato,Iwata Ozaki

Scientific reports 7:5816 PubMed28725041

2017

A screen for novel hepatitis C virus RdRp inhibitor identifies a broad-spectrum antiviral compound.

Applications

Unspecified application

Species

Unspecified reactive species

Abhilasha Madhvi,Smita Hingane,Rajpal Srivastav,Nishant Joshi,Chandru Subramani,Rajagopalan Muthumohan,Renu Khasa,Shweta Varshney,Manjula Kalia,Sudhanshu Vrati,Milan Surjit,C T Ranjith-Kumar

Oncotarget 8:56228-56242 PubMed28915586

2017

Hepatitis C virus core protein targets 4E-BP1 expression and phosphorylation and potentiates Myc-induced liver carcinogenesis in transgenic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Cosette Abdallah,Charlène Lejamtel,Nassima Benzoubir,Serena Battaglia,Nazha Sidahmed-Adrar,Christophe Desterke,Matthieu Lemasson,Arielle R Rosenberg,Didier Samuel,Christian Bréchot,Delphine Pflieger,François Le Naour,Marie-Françoise Bourgeade

Molecular & cellular proteomics : MCP 13:3040-8 PubMed25044019

2014

A novel immuno-competitive capture mass spectrometry strategy for protein-protein interaction profiling reveals that LATS kinases regulate HCV replication through NS5A phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Hélène Meistermann,Junjun Gao,Sabrina Golling,Jens Lamerz,Sophie Le Pogam,Manuel Tzouros,Sailaja Sankabathula,Lore Gruenbaum,Isabel Nájera,Hanno Langen,Klaus Klumpp,Angélique Augustin

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Hepatitis C Virus NS5a antibody [H26]

Western blot - Anti-Hepatitis C Virus NS5a antibody [H26] (AB13833)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Specificity

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (11)

Product promise