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AB322521

Anti-Hepatitis C Virus region NS5B antibody [1A4]

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Mouse Recombinant Monoclonal POLG antibody. Suitable for WB, ICC/IF and reacts with Transfected cell lysate - Hepatitis C virus, Transfected cell line - Hepatitis C virus samples.
2 이미지
Immunocytochemistry/ Immunofluorescence - Anti-Hepatitis C Virus region NS5B antibody [1A4] (AB322521)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Hepatitis C Virus region NS5B antibody [1A4] (AB322521)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Hepatitis C Virus region NS5B with ab322521 at 1/2000 (0.47 ug/ml) dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

Confocal image showing positive staining in 293T cells transfected with a Hepatitis C virus genotype 2a (isolate JFH-1) (HCV) region NS5B expression vector containing a myc-His-tag®(shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution, followed by ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 (2μg/ml) dilution (Magenta).

Western blot - Anti-Hepatitis C Virus region NS5B antibody [1A4] (AB322521)
  • WB

Supplier Data

Western blot - Anti-Hepatitis C Virus region NS5B antibody [1A4] (AB322521)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-Hepatitis C Virus region NS5B antibody [1A4] (ab322521) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-his tag, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a Hepatitis C virus genotype 2a (isolate JFH-1) (HCV) region NS5B expression vector containing a myc-his-tag, whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 65 kDa,36 kDa

false

Exposure time: 180s

관련 conjugated 항체와 다양한 조성의 항체 (1)

  • Carrier free

    Anti-Hepatitis C Virus region NS5B antibody [1A4] - BSA and Azide free

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

1A4

Isotype

IgG2a

Carrier free

No

Reacts with

Hepatitis C virus

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Primary Antibodies

AB190710

Anti-Human Nucleoli antibody [NM95] - Nucleolar Marker

BOND RX™ Validated|Recombinant|Lab Essentials

Immunohistochemistry - Anti-Human Nucleoli antibody [NM95] - Nucleolar Marker (AB190710)

  • 5
  • 2
Primary Antibodies

AB192022

Anti-NDUFS2 antibody [EPR16266]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS2 antibody [EPR16266] (AB192022)

  • 0
  • 0
Primary Antibodies

AB108986

Anti-PGP9.5 antibody [EPR4118] - Neuronal Marker

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Western blot - Anti-PGP9.5 antibody [EPR4118] - Neuronal Marker (AB108986)

  • 5
  • 12
Primary Antibodies

AB191181

Anti-Human Nuclear Antigen antibody [235-1]

Immunocytochemistry/ Immunofluorescence - Anti-Human Nuclear Antigen antibody [235-1] (AB191181)

  • 5
  • 4
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Hepatitis C virus": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell line - Hepatitis C virus": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/2000", "ICCIF-species-notes": "<p></p>" }, "Transfected cell lysate - Hepatitis C virus": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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제품 세부 정보

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

See full target information Hepatitis C virus NS5B

대체 명칭 보기

HCV NS5B, P68, RNA dependent RNA polymerase, RNA directed RNA polymerase
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com