AB308337

Anti-Hepatitis D virus Large delta + Small delta protein antibody [HL1053] - BSA and Azide free

Recombinant
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Rabbit Recombinant Monoclonal Hepatitis D Virus antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Transfected cell line - Hepatitis D virus, Transfected cell lysate - Hepatitis D virus samples.

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Immunocytochemistry/ Immunofluorescence - Anti-Hepatitis D virus Large delta + Small delta protein antibody [HL1053] - BSA and Azide free (AB308337)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Hepatitis D virus Large delta + Small delta protein antibody [HL1053] - BSA and Azide free (AB308337)

For immunofluorescence analysis, mock and transfected HEK293T cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of Hepatitis D virus Large delta Small delta protein using ab308337 at 1/500 dilution (green). Blue : Fluoroshield with DAPI.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1053

Isotype

IgG

Carrier free

Yes

Reacts with

Hepatitis D virus

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Hepatitis D virus": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell line - Hepatitis D virus": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell lysate - Hepatitis D virus": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" } } }

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제품 세부 정보

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Hepatitis D Virus (HDV) also known as delta virus is a small circular RNA virus. It is unique among viruses because it requires the presence of Hepatitis B Virus (HBV) for its replication and infection cycle. The delta protein encoded by the HDV RNA genome has an important role in its replication. The delta protein typically has a mass of approximately 24 kDa and is expressed specifically in the liver cells of infected individuals. Due to its dependence on HBV HDV is classified within the family of Deltaviridae.

Biological function summary

The delta protein interacts with host cellular machinery to facilitate HDV replication. It is part of a ribonucleoprotein complex that consists of the viral RNA and the delta protein itself. The complex enables the replication of the viral genome and packaging of the mature virus. The HDV RNA acts as both a template for replication and a ribozyme allowing it to self-cleave and process into the mature genomic RNA. The delta protein in its large form is essential for encapsidation with the HBV surface antigens completing the assembly of infectious virus particles.

Pathways

Hepatitis D virus follows a unique co-infective pathway relying heavily on the HBV lifecycle. The interaction between the HDV delta protein and HBV surface antigens particularly the large HBV surface antigen plays an important role in the assembly and secretion of new virions. HDV actively manipulates the host cellular pathways to facilitate its replication and requires the host's RNA polymerase II for synthesizing its RNA genome. The HBV-associated pathogenesis further accentuates liver damage caused by co-infection.

HDV infection significantly contributes to severe liver diseases including fulminant hepatitis and liver cirrhosis and it increases the risk of hepatocellular carcinoma in affected patients. The combined infection of HDV and HBV leads to a more aggressive disease course and the presence of HBV proteins especially the surface antigen is necessary for HDV propagation. Research into hepatitis D antibodies targets these viral proteins to develop novel therapeutic strategies and prevent liver disease progression.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Following virus entry into host cell, provides nuclear import of HDV RNPs thanks to its nuclear localization signal. Needs co-infection with hepatitis B virus to provide surface proteins, otherwise there is no packaging or budding. Packages the HDV ribonucleoprotein in hepatitis B virus empty particles. Interacts with both HDV genomic RNA and cytoplasmic tail of HBsAg. May inhibit viral RNA replication.

See full target information Large delta antigen

대체 명칭 보기

Large delta antigen, L-HDAg, p27, Small delta antigen, S-HDAg, p24

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Hepatitis D virus Large delta + Small delta protein antibody [HL1053] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-Hepatitis D virus Large delta + Small delta protein antibody [HL1053] - BSA and Azide free (AB308337)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Sample Prep & Detection Kits

Sample Prep & Detection Kits

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

Product promise