AB233244

Anti-Hepatitis E Virus antibody [5F3]

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(4 제품이 사용된 논문 )

Mouse Monoclonal Hepatitis E Virus antibody. Suitable for ELISA, ICC/IF, WB and reacts with Hepatitis E virus type 3 samples. Cited in 4 publications.

대체 명칭 보기

HEV

2 이미지

Immunocytochemistry/ Immunofluorescence - Anti-Hepatitis E Virus antibody [5F3] (AB233244)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Hepatitis E Virus antibody [5F3] (AB233244)

HEV-3-infected cells were fixed with acetone-methanol (1 : 1; 20 °C) and blocked with 1% FCS, then incubated with ab233244 at 1/50 dilution for 1 h at 37°C. FITC-conjugated anti-mouse IgG at 1/100 dilution was used as the secondary antibody. Cell nuclei counterstained with DAPI (Blue).

Supplier Data

Western blot - Anti-Hepatitis E Virus antibody [5F3] (AB233244)

M - PageRuler Prestained Protein Ladder

All lanes:

Western blot - Anti-Hepatitis E Virus antibody [5F3] (ab233244) at 1/1000 dilution

Lane 1:

Rat HEV capsid protein at 0.5 µg

Lane 2:

HEV genotype 3 at 0.5 µg

Lane 3:

Human parvovirus 4 VP2 protein at 0.5 µg

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주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

5F3

Isotype

IgG2b

Carrier free

Reacts with

Hepatitis E virus type 3

Applications

ELISA, WB, ICC/IF

applications

Specificity

This antibody recognizes the capsid protein of the HEV genotype 3. Does not react with rat HEV capsid protein.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Hepatitis E virus type 3": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "1/1000 - 1/10000", "ELISA-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/20 - 1/50", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.1% Sodium azide Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Hepatitis E virus (HEV) is a non-enveloped RNA virus with a genome that encodes three open reading frames. The mass of the virus particle has not been explicitly determined due to its virus nature but it comprises several proteins including the capsid protein which is essential for virion assembly. HEV predominantly expresses in the liver as this organ serves as the primary site for its replication. Commonly researchers refer to hepatitis E virus by its abbreviation HEV in scientific literature.

Biological function summary

HEV impacts liver function through its replication within hepatocytes contributing to liver inflammation and damage. The virus does not form a complex with other viral components but relies on host cell machinery for its replication cycle. Hepatitis E antibodies including anti-HEV and HEV antibodies such as 5f3 can target the virus for immune recognition and clearance with the immune response playing an important role in controlling and eliminating the infection.

Pathways

The virus integrates into the immune signaling pathways associated with viral recognition and response. HEV interacts with innate immune pathways to subvert the host's defense mechanisms. The interferon pathway is particularly significant in moderating the viral life cycle and proteins such as interferon-stimulated genes (ISGs) become upregulated in response to HEV presence. Additionally interactions with proteins within the apoptotic pathway may contribute to virus-induced cellular responses influencing both cell survival and antiviral defense.

HEV is primarily linked to hepatitis E an acute liver disease with symptoms such as jaundice fatigue and nausea. This condition can become more severe in pregnant women leading to maternal mortality. HEV infection can also escalate into chronic hepatitis particularly in immunocompromised individuals. In chronic cases the virus associates with proteins involved in the liver fibrosis process potentially contributing to long-term liver damage and cirrhosis. Current research continues to explore the relationship between hepatitis E antibodies and the modulation of disease outcomes.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

See full target information Hepatitis E Virus

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제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Acta biochimica et biophysica Sinica 56:1365-1372 PubMed38877781

2024

Hepatitis E virus infection upregulates ING5 expression and .

Applications

Unspecified application

Species

Unspecified reactive species

Wanqiu Zhao,Yueping Xia,Tengyuan Li,Huichan Liu,Guo Zhong,Dongxue Chen,Wenhai Yu,Yunlong Li,Fen Huang

Viruses 13: PubMed33924409

2021

Molecular Characterization and Seroprevalence of Hepatitis E Virus in Inflammatory Bowel Disease Patients and Solid Organ Transplant Recipients.

Applications

ICC/IF

Species

Human

Juozas Grigas,Maria Montoya,Evelina Simkute,Marius Buitkus,Ruta Zagrabskaite,Arnoldas Pautienius,Dainius Razukevicius,Laimas Virginijus Jonaitis,Gediminas Kiudelis,Jurgita Skieceviciene,Ruta Vaiciuniene,Asta Stankuviene,Inga Arune Bumblyte,Juozas Kupcinskas,Arunas Stankevicius

BMC veterinary research 16:95 PubMed32199460

2020

Hepatitis E genotype 3 virus isolate from wild boar is capable of replication in non-human primate and swine kidney cells and mouse neuroblastoma cells.

Applications

ICC/IF

Species

Unspecified reactive species

Juozas Grigas,Evelina Simkute,Martynas Simanavicius,Arnoldas Pautienius,Zaneta Streimikyte-Mockeliune,Dainius Razukevicius,Arunas Stankevicius

Applied microbiology and biotechnology 102:185-198 PubMed29143081

2017

Generation in yeast and antigenic characterization of hepatitis E virus capsid protein virus-like particles.

Applications

Unspecified application

Species

Unspecified reactive species

Martynas Simanavicius,Paulius Lukas Tamosiunas,Rasa Petraityte-Burneikiene,Reimar Johne,Rainer G Ulrich,Aurelija Zvirbliene,Indre Kucinskaite-Kodze

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Hepatitis E Virus antibody [5F3]

Immunocytochemistry/ Immunofluorescence - Anti-Hepatitis E Virus antibody [5F3] (AB233244)

Western blot - Anti-Hepatitis E Virus antibody [5F3] (AB233244)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Specificity

대체품

Primary Antibodies

보완 제품

Secondary Antibodies

Proteins & Peptides

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (4)

Product promise