AB319032

Anti-HERV-K-env antibody [EPR27172-7] - BSA and Azide free

Recombinant
RabMAb
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Rabbit Recombinant Monoclonal HERV-K-env antibody. Carrier free. Suitable for WB and reacts with Transfected cell lysate - Human, Human samples.

3 이미지

Supplier Data

Western blot - Anti-HERV-K-env antibody [EPR27172-7] - BSA and Azide free (AB319032)

This data was developed using ab319031, the same antibody clone in a different buffer formulation.

The expression of HERV-K-env is upregulated in response to estradiol treatment (PMID : 12629516).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-HERV-K-env antibody [EPR27172-7] (<a href='/ko/products/primary-antibodies/herv-k-env-antibody-epr27172-7-ab319031'>ab319031</a>) at 1/1000 dilution

Lane 1:

Untreated MCF7 (human breast adenocarcinoma epithelial cell) starved for 24 hours, whole cell lysate at 20 µg

Lane 2:

MCF7 starved for 24 hours, then treated with 10nM 17beta-Estradiol for 72 hours, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 75 kDa,36 kDa

false

Exposure time: 26s

Supplier Data

Western blot - Anti-HERV-K-env antibody [EPR27172-7] - BSA and Azide free (AB319032)

This data was developed using ab319031, the same antibody clone in a different buffer formulation.

Low expression : 22Rv1(PMID : 22247020), HEK-293.

The identity of the band beneath 75 kDa is unknown.

The expression profile is consistent with what has been described in the literature (PMID : 22247020).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-HERV-K-env antibody [EPR27172-7] (<a href='/ko/products/primary-antibodies/herv-k-env-antibody-epr27172-7-ab319031'>ab319031</a>) at 1/1000 dilution

Lane 1:

DU 145 (human prostate carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

22Rv1 (human prostate carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 75 kDa,36 kDa

false

Exposure time: 114s

Supplier Data

Western blot - Anti-HERV-K-env antibody [EPR27172-7] - BSA and Azide free (AB319032)

This data was developed using ab319031, the same antibody clone in a different buffer formulation.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-HERV-K-env antibody [EPR27172-7] (<a href='/ko/products/primary-antibodies/herv-k-env-antibody-epr27172-7-ab319031'>ab319031</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human HERV-K-env expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 75 kDa,124 kDa

false

Exposure time: 15s

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR27172-7

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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제품 세부 정보

ab319032 is the carrier-free version of ab319031.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

HERV-K-env also known as Human Endogenous Retrovirus K envelope protein has a mass of approximately 72 kDa. Mechanically HERV-K-env functions as a fusogenic protein facilitating the fusion of viral membranes with host cellular membranes. It is expressed in a variety of tissues with notable expression in germ cell tumor tissues and placental tissues. As a member of the envelope proteins in the HERV family it comprises part of the retroviral sequences integrated into the human genome.

Biological function summary

The envelope protein of the HERV-K family plays a role in cell-cell fusion and possibly in immune evasion. It is not part of a larger complex but interacts with cell surface receptors to mediate entry into cells. This function allows it to participate in the remodeling of cell membranes which relates to processes involved in early embryonic development and differentiation. Understanding its activity helps expand the knowledge on cellular transformation and tumorigenesis.

Pathways

HERV-K-env is involved in fusion and integration pathways. It engages in pathways associated with viral entry mechanisms akin to contemporary retroviruses. Notably it interrelates with pathways that include the interaction of viral proteins with host cell receptors sharing some mechanistic similarities with proteins such as Env from HIV. Through these pathways HERV-K-env influences the cellular environment in a manner that could redirect cellular signaling.

There is a connection between HERV-K-env expression and certain cancers including germ cell tumors and melanoma. Expression of HERV-K-env in these malignancies may contribute to oncogenic processes and immune evasion. Additionally it has links to autoimmune disorders potentially affecting proteins such as Toll-like receptors which are involved in immune system activation. Understanding its role in these diseases aids in developing potential therapeutic strategies targeting endogenous retroviral elements.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

See full target information HERV-K-env

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HERV-K-env antibody [EPR27172-7] - BSA and Azide free

Western blot - Anti-HERV-K-env antibody [EPR27172-7] - BSA and Azide free (AB319032)

Western blot - Anti-HERV-K-env antibody [EPR27172-7] - BSA and Azide free (AB319032)

Western blot - Anti-HERV-K-env antibody [EPR27172-7] - BSA and Azide free (AB319032)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

Product promise