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AB119776

Anti-Hes1 antibody [OTI2D2]

4

(4 리뷰들)

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(19 제품이 사용된 논문 )

Mouse Monoclonal HES1 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Transfected cell lysate, Human, Transfected cell line samples. Cited in 19 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human HES1.

대체 명칭 보기

BHLHB39, HL, HRY, HES1, Transcription factor HES-1, Class B basic helix-loop-helix protein 39, Hairy and enhancer of split 1, Hairy homolog, Hairy-like protein, bHLHb39, hHL

3 이미지
Flow Cytometry (Intracellular) - Anti-Hes1 antibody [OTI2D2] (AB119776)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Hes1 antibody [OTI2D2] (AB119776)

ab119776, at 1/100 dilution, staining Hes1 (red) in Jurkat cells, compared to a nonspecific negative control antibody,(blue) by Flow cytometry (Intracellular).

This image was generated using the ascites version of the product.

Western blot - Anti-Hes1 antibody [OTI2D2] (AB119776)
  • WB

Unknown

Western blot - Anti-Hes1 antibody [OTI2D2] (AB119776)

HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC211709)

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-Hes1 antibody [OTI2D2] (ab119776) at 1/2000 dilution

Lane 1:

HEK293T cell lysate transfected with pCMV6-ENTRY control cDNA at 5 µg

Lane 2:

HEK293T cell lysate transfected with pCMV6-ENTRY Hes1 cDNA at 5 µg

Predicted band size: 30 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-Hes1 antibody [OTI2D2] (AB119776)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Hes1 antibody [OTI2D2] (AB119776)

ab119776, at 1/100 dilution, staining Hes1 in COS7 cells transiently transfected with pCMV6-ENTRY Hes1 cDNA by Immunofluorescence.

This image was generated using the ascites version of the product.

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI2D2

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

ICC/IF, Flow Cyt (Intra), WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human HES1.

Q14469

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대체품

Primary Antibodies

AB108937

Anti-Hes1 antibody [EPR4226]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hes1 antibody [EPR4226] (AB108937)

  • 4
  • 15
Primary Antibodies

AB71559

Anti-Hes1 antibody

Western blot - Anti-Hes1 antibody (AB71559)

  • 4
  • 26
Primary Antibodies

AB196328

Alexa Fluor® 488 Anti-Hes1 antibody [EPR4226]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Hes1 antibody [EPR4226] (AB196328)

  • 0
  • 0
Primary Antibodies

AB196577

Alexa Fluor® 647 Anti-Hes1 antibody [EPR4226]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Hes1 antibody [EPR4226] (AB196577)

  • 0
  • 0
Primary Antibodies

AB221788

Anti-Hes1 antibody [EPR4226] - BSA and Azide free

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Hes1 antibody [EPR4226] - BSA and Azide free (AB221788)

  • 3
  • 1
Primary Antibodies

AB207048

Alexa Fluor® 594 Anti-Hes1 antibody [EPR4226]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Hes1 antibody [EPR4226] (AB207048)

  • 0
  • 0

보완 제품

Primary Antibodies

AB52627

Anti-Notch1 antibody [EP1238Y]

RabMAb|Recombinant|KO Validated|20ul selling size|Advanced Validation

Western blot - Anti-Notch1 antibody [EP1238Y] (AB52627)

  • 5
  • 18
Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

  • 5
  • 24
Primary Antibodies

AB23426

Anti-NOTCH3 antibody

BOND RX™ Validated

Western blot - Anti-NOTCH3 antibody (AB23426)

  • 4
  • 22
Primary Antibodies

AB154077

Anti-HEY1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-HEY1 antibody (AB154077)

  • 4
  • 2
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "<p><a href='/ko/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" }, "Transfected cell line": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Transfected cell lysate": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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제품 세부 정보

Dilute in PBS (pH7.3) before use.

This product was changed from ascites to tissue culture supernatant on the 4th of June 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

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특성 및 보관 정보

제형
Liquid
Purity
Tissue culture supernatant
Purification 관련 사항
Purified from TCS.
보관 버퍼
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Binds DNA on N-box motifs : 5'-CACNAG-3' with high affinity and on E-box motifs : 5'-CANNTG-3' with low affinity (By similarity). May play a role in a functional FA core complex response to DNA cross-link damage, being required for the stability and nuclear localization of FA core complex proteins, as well as for FANCD2 monoubiquitination in response to DNA damage.
See full target information HES1
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제품이 사용된 논문 (19)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell & bioscience 14:35 PubMed38494478

2024

Deubiquitinase PSMD7 facilitates pancreatic cancer progression through activating Nocth1 pathway via modifying SOX2 degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Luo,Yi Yu,Jinfeng Zhu,Leifeng Chen,Dan Li,Xingyu Peng,Zitao Liu,Qing Li,Qing Cao,Kai Huang,Rongfa Yuan

Arteriosclerosis, thrombosis, and vascular biology 44:653-665 PubMed38269590

2024

Single-Cell RNA-Seq Reveals Coronary Heterogeneity and Identifies CD133TRPV4 Endothelial Subpopulation in Regulating Flow-Induced Vascular Tone in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Aiqin Mao,Ka Zhang,Hao Kan,Mengru Gao,Zhiwei Wang,Tingting Zhou,Jing Shao,Dongxu He

Open medicine (Warsaw, Poland) 18:20230634 PubMed37082613

2023

F-box and WD repeat domain containing 7 inhibits the activation of hepatic stellate cells by degrading delta-like ligand 1 to block Notch signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yufeng Sun,Lili He,Peiran Guo,Fenghua Li,Bo Wang,Yifan Zhang,Kai An,Ming Peng

Nature communications 13:6117 PubMed36253375

2022

Brain milieu induces early microglial maturation through the BAX-Notch axis.

Applications

Unspecified application

Species

Unspecified reactive species

Fangying Zhao,Jiangyong He,Jun Tang,Nianfei Cui,Yanyan Shi,Zhifan Li,Shengnan Liu,Yazhou Wang,Ming Ma,Congjian Zhao,Lingfei Luo,Li Li

Oxidative medicine and cellular longevity 2022:7676872 PubMed36238644

2022

Lead Exposure Induced Neural Stem Cells Death via Notch Signaling Pathway and Gut-Brain Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Lijuan Sun,Yuankang Zou,Peng Su,Chong Xue,Diya Wang,Fang Zhao,Wenjing Luo,Jianbin Zhang

Cell death & disease 13:306 PubMed35383153

2022

Nogo-B promotes angiogenesis and improves cardiac repair after myocardial infarction via activating Notch1 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yanjun Zheng,Jingrong Lin,Dingsheng Liu,Guoqing Wan,Xuefeng Gu,Jian Ma

Journal of cell science 135: PubMed34982151

2022

High-content image analysis to study phenotypic heterogeneity in endothelial cell monolayers.

Applications

Unspecified application

Species

Unspecified reactive species

Francois Chesnais,Jonas Hue,Errin Roy,Marco Branco,Ruby Stokes,Aize Pellon,Juliette Le Caillec,Eyad Elbahtety,Matteo Battilocchi,Davide Danovi,Lorenzo Veschini

Journal of materials science. Materials in medicine 33:4 PubMed34940936

2021

Hydrogel contained valproic acid accelerates bone-defect repair via activating Notch signaling pathway in ovariectomized rats.

Applications

Unspecified application

Species

Unspecified reactive species

Zhou-Shan Tao,Tian-Lin Li,Hong-Guang Xu,Min Yang

Circulation. Genomic and precision medicine 14:e003142 PubMed33517678

2021

Whole-Transcriptome Profiling of Human Heart Tissues Reveals the Potential Novel Players and Regulatory Networks in Different Cardiomyopathy Subtypes of Heart Failure.

Applications

Unspecified application

Species

Unspecified reactive species

Chia-Feng Liu,Ying Ni,Christine S Moravec,Michael Morley,Euan A Ashley,Thomas P Cappola,Kenneth B Margulies,W H Wilson Tang

Cells 9: PubMed32993109

2020

Integrated Transcriptome and Proteome Analyses Reveal the Regulatory Role of miR-146a in Human Limbal Epithelium via Notch Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Adam J Poe,Mangesh Kulkarni,Aleksandra Leszczynska,Jie Tang,Ruchi Shah,Yasaman Jami-Alahmadi,Jason Wang,Andrei A Kramerov,James Wohlschlegel,Vasu Punj,Alexander V Ljubimov,Mehrnoosh Saghizadeh
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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