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AB117864

Anti-Hex antibody [OTI3E6]

4

(1 리뷰)

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(1 출판물)

Mouse Monoclonal Hex antibody. Suitable for WB, ICC/IF and reacts with Human, Transfected cell lysate samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human HHEX aa 100 to C-terminus.

대체 명칭 보기

HEX, PRH, PRHX, HHEX, Hematopoietically-expressed homeobox protein HHEX, Homeobox protein HEX, Homeobox protein PRH, Proline-rich homeodomain protein

3 이미지
Immunocytochemistry/ Immunofluorescence - Anti-Hex antibody [OTI3E6] (AB117864)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Hex antibody [OTI3E6] (AB117864)

HT-29 (human colorectal adenocarcinoma cell line) cells stained for Hex (green) using ab117864 at 1/100 dilution in ICC/IF.

Western blot - Anti-Hex antibody [OTI3E6] (AB117864)
  • WB

Unknown

Western blot - Anti-Hex antibody [OTI3E6] (AB117864)

All lanes:

Western blot - Anti-Hex antibody [OTI3E6] (ab117864) at 1/1000 dilution

Lane 1:

HepG2 (human liver hepatocellular carcinoma cell line) cell extract at 35 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract at 35 µg

Lane 3:

HT-29 (human colorectal adenocarcinoma cell line) cell extract at 35 µg

Lane 4:

A549 (human lung carcinoma cell line) cell extract at 35 µg

Lane 5:

COS-7 (african green monkey kidney fibroblast-like cell line) cell extract at 35 µg

Lane 6:

Jurkat (human T cell leukemia cell line from peripheral blood) cell extract at 35 µg

Lane 7:

MDCK (canine kidney cell line) cell extract at 35 µg

Lane 8:

PC-12 (rat adrenal gland pheochromocytoma cell line) cell extract at 35 µg

Lane 9:

MCF7 (human breast adenocarcinoma cell line) cell extract at 35 µg

Predicted band size: 30 kDa

false

Western blot - Anti-Hex antibody [OTI3E6] (AB117864)
  • WB

Unknown

Western blot - Anti-Hex antibody [OTI3E6] (AB117864)

All lanes:

Western blot - Anti-Hex antibody [OTI3E6] (ab117864) at 1/1000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control at 5 µg

Lane 2:

HEK-293T cells lysate transfected with pCMV6-ENTRY Hex cDNA at 5 µg

Predicted band size: 30 kDa

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI3E6

Isotype

IgG2b

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human HHEX aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q03014

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대체품

Primary Antibodies

AB153899

Anti-Hex antibody

Western blot - Anti-Hex antibody (AB153899)

  • 0
  • 0
Primary Antibodies

AB220764

Anti-Hex antibody

Immunocytochemistry/ Immunofluorescence - Anti-Hex antibody (AB220764)

  • 0
  • 0
Primary Antibodies

AB50836

Anti-Hex antibody [HHE5I261]

  • 0
  • 0

보완 제품

Primary Antibodies

AB52061

Anti-Cy5 antibody [CY5-15]

  • 3
  • 1
Primary Antibodies

AB420

Anti-Digoxigenin antibody [21H8]

  • 0
  • 0
Primary Antibodies

AB108422

Anti-FOXA2 antibody [EPR4466]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA2 antibody [EPR4466] (AB108422)

  • 4
  • 12
Primary Antibodies

AB236759

Anti-HNF-1B antibody [CL0374]

Immunocytochemistry/ Immunofluorescence - Anti-HNF-1B antibody [CL0374] (AB236759)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell lysate": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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제품 세부 정보

Clone OTI3E6 (formerly 3E6).
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification
Purification 관련 사항
Purified from cell culture supernatant by affinity chromatography.
보관 버퍼
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Recognizes the DNA sequence 5'-ATTAA-3' (By similarity). Transcriptional repressor (By similarity). Activator of WNT-mediated transcription in conjunction with CTNNB1 (PubMed : 20028982). Establishes anterior identity at two levels; acts early to enhance canonical WNT-signaling by repressing expression of TLE4, and acts later to inhibit NODAL-signaling by directly targeting NODAL (By similarity). Inhibits EIF4E-mediated mRNA nuclear export (PubMed : 12554669). May play a role in hematopoietic differentiation (PubMed : 8096636).
See full target information HHEX
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

The Journal of biological chemistry 300:107290 PubMed38636664

2024

Inability to rescue stalled ribosomes results in overactivation of the integrated stress response.

Applications

Unspecified application

Species

Unspecified reactive species

Ankanahalli N Nanjaraj Urs,Victor Lasehinde,Lucas Kim,Elesa McDonald,Liewei L Yan,Hani S Zaher
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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