Anti-HEY1 antibody

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HEY1 antibody (AB154077)

ab154077 staining HEY1 in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde. Samples were incubated with primary antibody (1/2000) (green). Alpha tubulin stained red and nuclei stained blue

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HEY1 antibody (AB154077)

ab154077 staining HEY1 in H1299 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde. Samples were incubated with primary antibody (1/1000) (green). Nuclei were stained blue with Hoechst 33342

• WB

Unknown

Western blot - Anti-HEY1 antibody (AB154077)

12% SDS PAGE

All lanes:

Western blot - Anti-HEY1 antibody (ab154077) at 1/1000 dilution

Lane 1:

A549 whole cell lysate at 30 µg

Lane 2:

H1299 whole cell lysate at 30 µg

Lane 3:

HCT116 whole cell lysate at 30 µg

Lane 4:

MCF-7 whole cell lysate at 30 µg

Predicted band size: 33 kDa

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• WB

CiteAb

Western blot - Anti-HEY1 antibody (AB154077)

HEY1 western blot using anti-HEY1 antibody ab154077. Publication image and figure legend from Jiang, S., Zhou, F., et al., 2020, J Cancer, PubMed 32284759.

ab154077 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab154077 please see the product overview.

HEY1 is a key factor in the tumorigenicity of 143B osteosarcoma cells. (A) Protein expression level of HEY1 in six osteosarcoma cell lines (MG-63, Saos-2, U-2 OS, MNNG/HOS, 143B, and SJSA-1). (B) Protein expression level of HEY1 after lentivirus-mediated RNA interference in 143B osteosarcoma cells. (C) Lentivirus infection efficiency detected by fluorescence microscopy (scale bar = 200 μm). (D) Lentivirus-infected 143B osteosarcoma cell pellet under visible light. (E) Images of 143B cell-bearing mice under 530-nm laser irradiation at the experimental endpoint. Subcutaneous tumors at the experimental endpoint (F), tumor growth curves (G), and tumor weight (H) are shown. (I) Protein expression level of HEY1 in Saos-2 and HEY1-overexpressed Saos-2 cells. (J) Tumorigenicity assay of Saos-2 cells after HEY1-overexpression. Subcutaneous tumors at the experimental endpoint (K), tumor growth curves (L), and tumor weight (M) of Saos-2 cells after HEY1-overexpression are shown.

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