Anti-HEY2 antibody [EPR29024-13]

• WB

Supplier Data

Western blot - Anti-HEY2 antibody [EPR29024-13] (AB323267)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-HEY2 antibody [EPR29024-13] (ab323267) at 1/1000 dilution

Lane 2:

HEK-293 transfected with siRNA specifically targeting HEY2 whole cell lysate at 80 µg

Lane 3:

HEK-293 (human embryonic kidney epithelial cell) transfected with scrambled siRNA control whole cell lysate at 80 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 37 kDa,36 kDa

false

Exposure time: 70s

• WB

Supplier Data

Western blot - Anti-HEY2 antibody [EPR29024-13] (AB323267)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lanes 1-2 : 48 seconds, lanes 3-4 : 180 seconds

All lanes:

Western blot - Anti-HEY2 antibody [EPR29024-13] (ab323267) at 1/1000 dilution

Lane 1:

Untreated HEK-293 whole cell lysate at 80 µg

Lane 2:

HEK-293 treated with 10 µM MG-132 for 6 hours whole cell lysate at 80 µg

Lane 3:

Untreated Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 80 µg

Lane 4:

Neuro-2a treated with 10 µM MG-132 for 16 hours whole cell lysate at 80 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 37 kDa,36 kDa

false

• WB

Supplier Data

Western blot - Anti-HEY2 antibody [EPR29024-13] (AB323267)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human HEY1 and HEYL.

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-6X His tag® antibody [EPR20547] - Total Protein Control ChIP Grade (ab213204) at 1/5000 dilution.

All lanes:

Western blot - Anti-HEY2 antibody [EPR29024-13] (ab323267) at 1/1000 dilution

Lane 1:

His-tagged human HEY2 recombinant protein at 10 ng

Lane 2:

His-tagged human HEY1 recombinant protein at 10 ng

Lane 3:

His-tagged human HEYL recombinant protein at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 37 kDa,90 kDa

false

Exposure time: 180s