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AB244666

Anti-HGF antibody [EPR20611-16] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal HGF antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples.

대체 명칭 보기

Hepatocyte growth factor, Hepatopoietin-A, Scatter factor, SF, Hgf

1 이미지
Sandwich ELISA - Anti-HGF antibody [EPR20611-16] - BSA and Azide free (Capture) (AB244666)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-HGF antibody [EPR20611-16] - BSA and Azide free (Capture) (AB244666)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab223862).

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20611-16

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Assay Kits

AB275901

Human HGF ELISA Kit (Hepatocyte Growth Factor)

Recombinant|SimpleStep

ELISA - Human HGF ELISA Kit (Hepatocyte Growth Factor) (AB275901)

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Primary Antibodies

AB259472

Anti-MIP-3 beta/CCL19 antibody [EPR21931-34] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-MIP-3 beta/CCL19 antibody [EPR21931-34] - BSA and Azide free (Detector) (AB259472)

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Primary Antibodies

AB242709

Anti-CXCL2 antibody [EPR17014-241] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-CXCL2 antibody [EPR17014-241] - BSA and Azide free (Detector) (AB242709)

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Primary Antibodies

AB242491

Anti-IL-10 antibody [EPR17277-261] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-IL-10 antibody [EPR17277-261] - BSA and Azide free (Capture) (AB242491)

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  • 0
Primary Antibodies

AB267436

Anti-LIX antibody [EPR22392-51] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-LIX antibody [EPR22392-51] - BSA and Azide free (Detector) (AB267436)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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제품 세부 정보

ab244666 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse HGF. The recommended pair for sandwich ELISA is:
Capture: ab244666, Mouse HGF Capture Antibody (unconjugated)
Detector: ab244931, Mouse HGF Detector Antibody (unconjugated)
The reference range value is 62.5 - 4000 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The hepatocyte growth factor (HGF) also known as scatter factor (SF) is a protein with a mass of approximately 83 kDa. This protein is mostly expressed in the liver but it can also be found in other organs such as the kidney and the lungs. HGF acts mechanically by binding to the c-Met receptor activating intracellular signaling mechanisms necessary for various physiological processes. Researchers often use HGF ELISA kits to measure its levels in biological samples and study its various roles.
Biological function summary

HGF plays an essential role in cellular growth regeneration and healing processes across different tissues. It is not part of a complex but functions as a single-chain polypeptide that undergoes cleavage into its active form. HGF facilitates cell proliferation and migration; this is especially important during organ regeneration and repair processes. By engaging with its receptor c-Met it induces cell survival and cytoskeletal reorganization making it important for wound healing.

Pathways

HGF is critical in the MAPK and PI3K-Akt signaling pathways which impact processes like cell division and survival. Through these pathways HGF interacts with other proteins such as ERK and Akt contributing to the regulation of cellular growth and metabolism. HGF's role in these pathways highlights its importance in developmental processes and tissue homeostasis.

HGF displays significant connections to cancer and liver cirrhosis. Its overexpression can lead to the development of various carcinomas due to its effect on cell proliferation and migration. In liver cirrhosis the altered HGF-c-Met signaling might contribute to the disease progression and hepatic dysfunction. Additionally HGF can interact with proteins such as transforming growth factor-beta (TGF-β) in these conditions influencing the pathological environment.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types (PubMed : 20624990). Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization (By similarity). Activates MAPK signaling following TMPRSS13 cleavage and activation (By similarity).
See full target information Hgf
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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