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AB313408

Anti-HGF antibody [RP23040069]

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Rabbit Recombinant Multiclonal HGF antibody. Suitable for ICC/IF, WB, I-ELISA and reacts with Human, Transfected cell lysate - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human HGF.
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Immunocytochemistry/ Immunofluorescence - Anti-HGF Antibody [RP23040069] (AB313408)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HGF Antibody [RP23040069] (AB313408)

Immunofluorescent analysis of 70% confluent log phase U-87 MG cells fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature labeling HGF with ab313408 at 1 µg/mL concentration followed by Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor® 488 at 1/2000 dilution, for 45 minutes at room temperature (Panel a : Green). Nuclei (Panel b : Blue) were stained with DAPI. F-actin (Panel c : Red) was stained with Rhodamine Phalloidin at 1/300 dilution. Panel d represents the merged image showing cytoplasmic localization. Panel e represents H1299 cells lacking the expression of HGF. Panel f represents control U-87 MG cells with no primary antibody to assess background. The images were captured at 60X magnification.

Immunocytochemistry/ Immunofluorescence - Anti-HGF Antibody [RP23040069] (AB313408)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HGF Antibody [RP23040069] (AB313408)

Immunofluorescent analysis of HepG2 cells fixed with 4% formaldehyde reconstituted in 1X PBS for 10 min at room temperature and permeabilized with 0.1 % Triton X-100 in PBS for 15 min at room temperature labeling HGF with ab313408 followed by Alexa Fluor® 488-conjugated Goat anti-Rabbit secondary antibody (green) (Image A). Nuclei were stained using DAPI (Image B) and actin stained with Alexa Fluor® 594 phalloidin (red) (image C). Image D is a composite image showing nuclear localization of HGF.

Indirect ELISA - Anti-HGF Antibody [RP23040069] (AB313408)
  • I-ELISA

Supplier Data

Indirect ELISA - Anti-HGF Antibody [RP23040069] (AB313408)

Indirect ELISA analysis of endogenous HGF in MCF-7 lysate coated onto the plate using ab313408 at various dilutions. A non-linear regression analysis was performed (4 PL) and LOD and LOQ for the antibody were determined.

Western blot - Anti-HGF Antibody [RP23040069] (AB313408)
  • WB

Supplier Data

Western blot - Anti-HGF Antibody [RP23040069] (AB313408)

All lanes:

Western blot - Anti-HGF Antibody [RP23040069] (ab313408) at 1 µg/mL

All lanes:

HepG2 cell lysate

Observed band size: 69 kDa

true

Western blot - Anti-HGF Antibody [RP23040069] (AB313408)
  • WB

Supplier Data

Western blot - Anti-HGF Antibody [RP23040069] (AB313408)

All lanes:

Western blot - Anti-HGF Antibody [RP23040069] (ab313408) at 1 µg/mL

Lane 1:

Non-transfected U-87 MG cell lysate

Lane 2:

Non-targeting scrambled siRNA transfected U-87 MG cell lysate

Lane 3:

Hepatocyte growth factor knockdown U-87 MG cell lysate

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/4000 dilution

Observed band size: 83 kDa

false

주요 정보

Host species

Rabbit

Clonality

Multiclonal

Clone number

RP23040069

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, I-ELISA, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human HGF.

P14210

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보완 제품

Assay Kits

AB275901

Human HGF ELISA Kit (Hepatocyte Growth Factor)

Recombinant|SimpleStep

ELISA - Human HGF ELISA Kit (Hepatocyte Growth Factor) (AB275901)

  • 0
  • 0
Primary Antibodies

AB208687

Anti-FGF2 antibody [EPR20145-219]

20ul selling size|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-FGF2 antibody [EPR20145-219] (AB208687)

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Primary Antibodies

AB109374

Anti-Topoisomerase I antibody [EPR5375]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase I antibody [EPR5375] (AB109374)

  • 4
  • 9
Assay Kits

AB246531

Human FGF2 ELISA Kit

Recombinant|SimpleStep

ELISA - Human FGF2 ELISA Kit (AB246531)

  • 4
  • 1
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "<p></p>", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p></p>" }, "Transfected cell lysate - Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "<p></p>", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" } } }
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제품 세부 정보

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
Preservative: 0.09% Sodium azide Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The hepatocyte growth factor (HGF) also known as scatter factor (SF) is a protein with a mass of approximately 83 kDa. This protein is mostly expressed in the liver but it can also be found in other organs such as the kidney and the lungs. HGF acts mechanically by binding to the c-Met receptor activating intracellular signaling mechanisms necessary for various physiological processes. Researchers often use HGF ELISA kits to measure its levels in biological samples and study its various roles.
Biological function summary

HGF plays an essential role in cellular growth regeneration and healing processes across different tissues. It is not part of a complex but functions as a single-chain polypeptide that undergoes cleavage into its active form. HGF facilitates cell proliferation and migration; this is especially important during organ regeneration and repair processes. By engaging with its receptor c-Met it induces cell survival and cytoskeletal reorganization making it important for wound healing.

Pathways

HGF is critical in the MAPK and PI3K-Akt signaling pathways which impact processes like cell division and survival. Through these pathways HGF interacts with other proteins such as ERK and Akt contributing to the regulation of cellular growth and metabolism. HGF's role in these pathways highlights its importance in developmental processes and tissue homeostasis.

HGF displays significant connections to cancer and liver cirrhosis. Its overexpression can lead to the development of various carcinomas due to its effect on cell proliferation and migration. In liver cirrhosis the altered HGF-c-Met signaling might contribute to the disease progression and hepatic dysfunction. Additionally HGF can interact with proteins such as transforming growth factor-beta (TGF-β) in these conditions influencing the pathological environment.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types (PubMed : 20624990). Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization (PubMed : 15167892, PubMed : 20977675). Activates MAPK signaling following TMPRSS13 cleavage and activation (PubMed : 20977675).
See full target information HGF

대체 명칭 보기

HPTA, HGF, Hepatocyte growth factor, Hepatopoietin-A, Scatter factor, SF
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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