Anti-HIF-2-alpha antibody [ep190b]

• WB

Unknown

Western blot - Anti-HIF-2-alpha antibody [ep190b] (AB8365)

HIF-2-alpha detected in hypoxic Human lysate using ab8365. Lane 1 : normoxic A549 lysate control, lane 2 : hypoxic A549 lysate.

All lanes:

Western blot - Anti-HIF-2-alpha antibody [ep190b] (ab8365)

Predicted band size: 96 kDa

false

• WB

Lab

Western blot - Anti-HIF-2-alpha antibody [ep190b] (AB8365)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 55 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab8365 overnight at 4°C. Antibody binding was detected using a Goat anti-mouse antibody conjugated to HRP, and visualised using ECL development solution ab133406.

All lanes:

Western blot - Anti-HIF-2-alpha antibody [ep190b] (ab8365) at 1/500 dilution

Lane 1:

HeLa whole cell lysate at 20 µg

Lane 2:

HeLa treated with 0.5mM DFO whole cell lysate at 20 µg

Secondary

All lanes:

HRP conjugated Goat Anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 96 kDa

Observed band size: 100 kDa

true

Exposure time: 20min