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AB237544

Anti-HIF1AN/FIH-1 antibody

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(7 제품이 사용된 논문 )

Rabbit Polyclonal HIF1AN/FIH-1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HIF1AN aa 1-300.

대체 명칭 보기

FIH1, HIF1AN, Hypoxia-inducible factor 1-alpha inhibitor, Factor inhibiting HIF-1, Hypoxia-inducible factor asparagine hydroxylase, FIH-1

1 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF1AN/FIH-1 antibody (AB237544)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF1AN/FIH-1 antibody (AB237544)

Paraffin-embedded human kidney tissue stained for HIF1AN/FIH-1 with ab237544 at 1/100 dilution in immunohistochemical analysis.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human HIF1AN aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

Q9NWT6

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대체품

Primary Antibodies

AB187524

Anti-HIF1AN/FIH-1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-HIF1AN/FIH-1 antibody (AB187524)

  • 0
  • 0
Primary Antibodies

AB233141

Anti-HIF1AN/FIH-1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF1AN/FIH-1 antibody (AB233141)

  • 0
  • 0
Primary Antibodies

AB92304

Anti-HIF1AN/FIH-1 antibody [EPR3659]

RabMAb|Recombinant|Trial Size

Flow Cytometry (Intracellular) - Anti-HIF1AN/FIH-1 antibody [EPR3659] (AB92304)

  • 0
  • 0
Primary Antibodies

AB92498

Anti-HIF1AN/FIH-1 antibody [EPR3658]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF1AN/FIH-1 antibody [EPR3658] (AB92498)

  • 0
  • 0

보완 제품

Primary Antibodies

AB179483

Anti-HIF-1 alpha antibody [EPR16897]

20ul selling size|RabMAb|Advanced Validation|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-HIF-1 alpha antibody [EPR16897] (AB179483)

  • 4
  • 9
Primary Antibodies

AB24982

Cy5 ® Anti-CD45.1 antibody [A20]

  • 0
  • 0
Primary Antibodies

AB172730

Rabbit IgG, monoclonal [EPR25A] - Isotype Control

RabMAb|Advanced Validation|Recombinant

Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

  • 0
  • 0
Primary Antibodies

AB38449

Anti-AKT (phospho T308) antibody

Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT (phospho T308) antibody (AB38449)

  • 3
  • 5
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein G
Purification 관련 사항
Purity >95%.
보관 버퍼
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

HIF1AN also known as FIH-1 (Factor Inhibiting HIF-1) is an enzyme primarily engaged in the hydroxylation of asparagine residues on hypoxia-inducible factor (HIF) alpha subunits. This action negatively regulates HIF by inhibiting its transcriptional activity. It has an approximate molecular weight of 40 kDa. HIF1AN is ubiquitously expressed in various tissues reflecting its critical role in cellular oxygen homeostasis.
Biological function summary

HIF1AN acts as an important regulator under normoxic conditions by maintaining the HIF complex in its inactive state. It does not operate alone; instead it is part of a larger protein complex that includes the HIF-alpha subunits and pVHL the latter marking HIF for degradation. This regulation ensures that the cellular response to oxygen levels is tightly controlled preventing unwarranted expression of hypoxia-related genes when oxygen is sufficient.

Pathways

The role of HIF1AN is integral in the HIF signaling and cellular response to hypoxia pathways. It is particularly associated with the modulation of the HIF pathway through its interaction with proteins such as HIF-1α and VHL. This relationship ensures a controlled activation of pathways that respond to low oxygen scenarios affecting processes like angiogenesis. The interactions between HIF1AN VHL and HIF-1α demonstrate HIF1AN's pivotal role in regulating gene expression linked to oxygen levels.

HIF1AN has connections to conditions like cancer and ischemic diseases. Its regulatory effects on HIF activity mean alterations in HIF1AN expression or function can contribute to tumor growth due to its role in promoting angiogenesis and metabolic adaptation in hypoxic tumor microenvironments. HIF1AN also has indirect links to the tumor suppressor protein VHL with mutations in VHL leading to aberrations in HIF-1α regulation further implicating HIF1AN in oncogenesis.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Hydroxylates HIF-1 alpha at 'Asn-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation.
See full target information HIF1AN
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제품이 사용된 논문 (7)

Recent publications for all applications. Explore the 전체 목록 and refine your search

International journal of stem cells 18:146-157 PubMed40059075

2025

PML Regulated HIF1AN Ubiquitination and Activated PI3K/AKT Pathway to Promote Bone Marrow Mesenchymal Stem Cells Osteogenic Differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Xian-Pei Zhou,Qi-Wei Li,Zi-Zhen Shu,Yang Liu

Frontiers in immunology 13:976482 PubMed36119083

2022

Ion channel Piezo1 activation promotes aerobic glycolysis in macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Shaoqiu Leng,Xiaoyu Zhang,Shuwen Wang,Jing Qin,Qiang Liu,Anli Liu,Zi Sheng,Qi Feng,Xiang Hu,Jun Peng

Journal of inflammation research 15:4171-4184 PubMed35912402

2022

High Expression of DEPDC1B Predicts Poor Prognosis in Lung Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Pulin Li,Xiaojuan Chen,Sijing Zhou,Xingyuan Xia,Enze Wang,Rui Han,Daxiong Zeng,Guanghe Fei,Ran Wang

PeerJ 10:e13370 PubMed35529504

2022

HIF1 is dispensable for oocyte development and female fertility in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yujia Chen,Siyu Du,Zhenyue Huang,Longsen Han,Qiang Wang

Renal failure 44:293-303 PubMed35166173

2022

MicroRNA-122-5p ameliorates tubular injury in diabetic nephropathy via FIH-1/HIF-1α pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Li Cheng,Xinying Qiu,Liyu He,Li Liu

Oxidative medicine and cellular longevity 2022:8548461 PubMed35069980

2022

lncRNA SNHG15 Induced by SOX12 Promotes the Tumorigenic Properties and Chemoresistance in Cervical Cancer via the miR-4735-3p/HIF1a Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jiang Yang,Mei Yang,Huabing Lv,Min Zhou,Xiaogang Mao,Xiaomin Qin,Ying Xu,Lin Li,Hui Xing

Molecular medicine reports 23: PubMed33495813

2021

miR‑212 promotes renal interstitial fibrosis by inhibiting hypoxia‑inducible factor 1‑α inhibitor.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Zhang,Guo-Xin Zhang,Li-Shuang Che,Shu-Han Shi,Yue-Ting Li
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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