AB11175

Anti-Histone H2A.X antibody

Name: Anti-Histone H2A.X antibody
Brand: Abcam Limited
SKU: AB11175
Rating: 5 (43 reviews)

(43 리뷰들)

(182 제품이 사용된 논문 )

Anti-Histone H2A.X antibody (ab11175) is a rabbit polyclonal antibody detecting Histone H2A.X in Western Blot, IHC-P. Suitable for Human, Mouse.

- Over 150 publications
- Trusted since 2004

대체 명칭 보기

H2AFX, H2AX, Histone H2AX, H2a/x, Histone H2A.X

3 이미지

Supplier Data

Western blot - Anti-Histone H2A.X antibody (AB11175)

Detection : Chemiluminescence

All lanes:

Western blot - Anti-Histone H2A.X antibody (ab11175) at 0.1 µg/mL

Lane 1:

F9 cell lysate (prepared using RIPA lysis buffer) at 50 µg

Lane 2:

L1210 cell lysate (prepared using RIPA lysis buffer) at 50 µg

Predicted band size: 15 kDa

false

Exposure time: 30s

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A.X antibody (AB11175)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A.X antibody (AB11175)

mmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling H2A.X with ab11175 at 1 μg/mL.

Unknown

Western blot - Anti-Histone H2A.X antibody (AB11175)

All lanes:

Western blot - Anti-Histone H2A.X antibody (ab11175) at 0.1 µg/mL

All lanes:

HEK293T cells at 50 µg

Predicted band size: 15 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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제품 세부 정보

What is this antibody validated in?
Anti-Histone H2A.X antibody (ab11175) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human, Mouse samples.

Trusted by the scientific community
Anti-Histone H2A.X (ab11175) was first used in a scientific publication in 2004 and has been cited over 150 times in peer-reviewed journals.

Reviewed by scientists
Anti-Histone H2A.X (ab11175) has over 40 independent reviews from customers.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

Antibodies were affinity purified using the peptide immobilized on solid support.

보관 버퍼

pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: Tris citrate/phosphate

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle|Do Not Freeze

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

See full target information H2AX

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제품이 사용된 논문 (182)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Epigenetics & chromatin 18:46 PubMed40665417

2025

H2A.X N-terminal acetylation is a newly identified NAA40-mediated modification that is responsive to UV irradiation.

Applications

Unspecified application

Species

Unspecified reactive species

Ariel Klavaris,Costas Koufaris,Roberta Noberini,Maria Kouma,Christina Demetriadou,Alessandro Ghiringhelli,Nikolas Dietis,Tiziana Bonaldi,Antonis Kirmizis

Genes to cells : devoted to molecular & cellular mechanisms 30:e70031 PubMed40452456

2025

PRMT4/CARM1 Is a Novel Factor Promoting DNA Double-Strand Break Repair.

Applications

Unspecified application

Species

Unspecified reactive species

Yurina Abe,Hayaki Ikegame,Yuina Tsuchiya,Ryotaro Nishi

Oncogene 44:1375-1386 PubMed39994376

2025

Cyclin E1 overexpression sensitizes ovarian cancer cells to WEE1 and PLK1 inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Xi,Akiko Kunita,Miho Ogawa,Mirei Ka,Saki Tanimoto,Saki Tsuchimochi,Saeko Nagai,Asami Matsunaga,Tomohiko Fukuda,Kousuke Watanabe,Kenbun Sone,Aya Shinozaki-Ushiku,Kei Kawana,Tetsuo Ushiku,Yutaka Osuga,Kazuhiro Katayama,Hidenori Kage,Katsutoshi Oda

Nature communications 15:9171 PubMed39448645

2024

Epigenetic modulation via the C-terminal tail of H2A.Z.

Applications

Unspecified application

Species

Unspecified reactive species

László Imre,Péter Nánási,Ibtissem Benhamza,Kata Nóra Enyedi,Gábor Mocsár,Rosevalentine Bosire,Éva Hegedüs,Erfaneh Firouzi Niaki,Ágota Csóti,Zsuzsanna Darula,Éva Csősz,Szilárd Póliska,Beáta Scholtz,Gábor Mező,Zsolt Bacsó,H T Marc Timmers,Masayuki Kusakabe,Margit Balázs,György Vámosi,Juan Ausio,Peter Cheung,Katalin Tóth,David Tremethick,Masahiko Harata,Gábor Szabó

European thyroid journal 13: PubMed39047147

2024

A post-irradiation-induced replication stress promotes RET proto-oncogene breakage.

Applications

Unspecified application

Species

Unspecified reactive species

Fabio Hecht,Laura Valerio,Carlos Frederico Lima Gonçalves,Marylin Harinquet,Rabii Ameziane El Hassani,Denise P Carvalho,Stephane Koundrioukoff,Jean-Charles Cadoret,Corinne Dupuy

International journal of molecular medicine 53: PubMed38695243

2024

Predictive DNA damage signaling for low‑dose ionizing radiation.

Applications

Unspecified application

Species

Unspecified reactive species

Jeong-In Park,Seung-Youn Jung,Kyung-Hee Song,Dong-Hyeon Lee,Jiyeon Ahn,Sang-Gu Hwang,In-Su Jung,Dae-Seog Lim,Jie-Young Song

Biology of reproduction 111:483-495 PubMed38625059

2024

Trajectory of primordial follicle depletion is accelerated in obese mice in response to 7,12-dimethylbenz[a]anthracene exposure†.

Applications

Unspecified application

Species

Unspecified reactive species

Jaspreet K Rishi,Kelsey Timme,Hunter E White,Karl C Kerns,Aileen F Keating

Journal of radiation research 65:263-271 PubMed38461549

2024

Inhibition of intracellular ATP synthesis impairs the recruitment of homologous recombination factors after ionizing radiation.

Applications

Unspecified application

Species

Unspecified reactive species

Ryota Hayashi,Hikaru Okumura,Mayu Isono,Motohiro Yamauchi,Daiki Unami,Rahmartani Tania Lusi,Masamichi Yamamoto,Yu Kato,Yuki Uchihara,Atsushi Shibata

The Journal of biological chemistry 300:105538 PubMed38072046

2023

Transcriptional regulation of FACT involves Coordination of chromatin accessibility and CTCF binding.

Applications

Unspecified application

Species

Unspecified reactive species

Peijun Wang,Na Fan,Wanting Yang,Pengbo Cao,Guojun Liu,Qi Zhao,Pengfei Guo,Xihe Li,Xinhua Lin,Ning Jiang,Buhe Nashun

Journal of thoracic disease 15:3350-3358 PubMed37426130

2023

Overexpressed collaborates with MMB to increase inhibitor sensitivity in NSCLC.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Tan,Jingbo Ma,Weiqiang Zhang,Jing Zhao,Keqiang Liu

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H2A.X antibody

Western blot - Anti-Histone H2A.X antibody (AB11175)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A.X antibody (AB11175)

Western blot - Anti-Histone H2A.X antibody (AB11175)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

제품이 사용된 논문 (182)

Product promise