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AB140498

Anti-Histone H2A.X antibody

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(4 제품이 사용된 논문 )

Goat Polyclonal H2A.X antibody. Suitable for IHC-P, WB and reacts with Mouse, Human samples. Cited in 4 publications.

대체 명칭 보기

H2AFX, H2AX, Histone H2AX, H2a/x, Histone H2A.X

3 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A.X antibody (AB140498)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A.X antibody (AB140498)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung cancer tissue labelling Histone H2A.X with ab140498 at 1/5000 (0.2µg/ml). Detection : DAB.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A.X antibody (AB140498)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A.X antibody (AB140498)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse renal cell carcinoma tissue labelling Histone H2A.X with ab140498 at 1/1000 (1µg/ml). Detection : DAB.

Western blot - Anti-Histone H2A.X antibody (AB140498)
  • WB

Unknown

Western blot - Anti-Histone H2A.X antibody (AB140498)

All lanes:

Western blot - Anti-Histone H2A.X antibody (ab140498) at 0.4 µg/mL

Lane 1:

293T whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 15 µg

Predicted band size: 15 kDa

true

Exposure time: 30s

주요 정보

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/5000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/5000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
Purification 관련 사항
ab140498 was affinity purified using an epitope specific to Histone H2A.X immobilized on a solid support.
보관 버퍼
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle|Do Not Freeze

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

타겟 정보

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
See full target information H2AX

제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature communications 13:6228 PubMed36266274

2022

A BDNF-TrkB autocrine loop enhances senescent cell viability.

Applications

Unspecified application

Species

Unspecified reactive species

Carlos Anerillas,Allison B Herman,Rachel Munk,Amanda Garrido,Kwan-Wood Gabriel Lam,Matthew J Payea,Martina Rossi,Dimitrios Tsitsipatis,Jennifer L Martindale,Yulan Piao,Krystyna Mazan-Mamczarz,Jinshui Fan,Chang-Yi Cui,Supriyo De,Kotb Abdelmohsen,Rafael de Cabo,Myriam Gorospe

Cells 8: PubMed31752383

2019

Acetylshikonin Sensitizes Hepatocellular Carcinoma Cells to Apoptosis through ROS-Mediated Caspase Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Hong,Jinke Li,Siying Li,Mohammed M Almutairi

Developmental cell 50:509-524.e10 PubMed31327739

2019

PI31 Is an Adaptor Protein for Proteasome Transport in Axons and Required for Synaptic Development.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Liu,Sandra Jones,Adi Minis,Jose Rodriguez,Henrik Molina,Hermann Steller

Cancer research 79:819-829 PubMed30573522

2018

EGFR and c-MET Cooperate to Enhance Resistance to PARP Inhibitors in Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Qiongzhu Dong,Yi Du,Hui Li,Chunxiao Liu,Yongkun Wei,Mei-Kuang Chen,Xixi Zhao,Yu-Yi Chu,Yufan Qiu,Lunxiu Qin,Hirohito Yamaguchi,Mien-Chie Hung
제품이 사용된 논문 모두 보기

Product promise

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