AB209980

Anti-Histone H2A.X antibody

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Rabbit Polyclonal H2A antibody. Suitable for IHC - Wmt, WB and reacts with Zebrafish samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Zebrafish h2ax aa 100 to C-terminus.

대체 명칭 보기

zgc:56329, h2ax, Histone H2AX, H2a/x, Histone H2A.X

5 이미지

IHC - Wholemount - Anti-Histone H2A.X antibody (AB209980)

IHC - Wmt

Supplier Data

IHC - Wholemount - Anti-Histone H2A.X antibody (AB209980)

ab209980 detects Histone H2A.X protein on whole mount 2 day-post-fertilization zebrafish embryo by immunohistochemical analysis at 1/100 dilution.

IHC - Wmt

Supplier Data

IHC - Wholemount - Anti-Histone H2A.X antibody (AB209980)

ab209980 detects Histone H2A.X protein on whole mount 2 day-post-fertilization zebrafish embryo by immunohistochemical analysis at 1/100 dilution.

IHC - Wmt

Supplier Data

IHC - Wholemount - Anti-Histone H2A.X antibody (AB209980)

Whole mount immunohistochemical analysis of paraformaldehyde-fixed zebrafish embryos labeling Histone H2A.X with ab209980 at 1/200 dilution.

Supplier Data

Western blot - Anti-Histone H2A.X antibody (AB209980)

Samples were separated by 15% SDS PAGE.

All lanes:

Western blot - Anti-Histone H2A.X antibody (ab209980) at 1/1000 dilution

All lanes:

Zebrafish embryos extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 15 kDa

false

Supplier Data

Western blot - Anti-Histone H2A.X antibody (AB209980)

Samples were separated by 15% SDS PAGE.

All lanes:

Western blot - Anti-Histone H2A.X antibody (ab209980) at 1/1000 dilution

Lane 1:

Un-treated 3dpf zebrafish embryos whole cell extracts at 30 µg

Lane 2:

treated 3dpf zebrafish embryos whole cell extracts at 30 µg

Predicted band size: 15 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Zebrafish

Applications

WB, IHC - Wmt

applications

Immunogen

Synthetic Peptide within Zebrafish h2ax aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q7ZUY3

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCWmt" : {"fullname" : "IHC - Wholemount", "shortname":"IHC - Wmt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Zebrafish": { "IHCWmt-species-checked": "testedAndGuaranteed", "IHCWmt-species-dilution-info": "1/100 - 1/500", "IHCWmt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }

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제품 세부 정보

Non-phospho Ser139

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle|Store undiluted

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation (By similarity).

See full target information h2ax

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

The EMBO journal 43:5635-5666 PubMed39349846

2024

Carboxy-terminal polyglutamylation regulates signaling and phase separation of the Dishevelled protein.

Applications

Unspecified application

Species

Unspecified reactive species

Marek Kravec,Ondrej Šedo,Jana Nedvědová,Miroslav Micka,Marie Šulcová,Nikodém Zezula,Kristína Gömöryová,David Potěšil,Ranjani Sri Ganji,Sara Bologna,Igor Červenka,Zbyněk Zdráhal,Jakub Harnoš,Konstantinos Tripsianes,Carsten Janke,Cyril Bařinka,Vítězslav Bryja

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H2A.X antibody

IHC - Wholemount - Anti-Histone H2A.X antibody (AB209980)

IHC - Wholemount - Anti-Histone H2A.X antibody (AB209980)

IHC - Wholemount - Anti-Histone H2A.X antibody (AB209980)

Western blot - Anti-Histone H2A.X antibody (AB209980)

Western blot - Anti-Histone H2A.X antibody (AB209980)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise