AB240613

Anti-Histone H2B (acetyl K11) antibody

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(2 제품이 사용된 논문 )

Rabbit Polyclonal H2B acetyl K11 antibody. Suitable for ICC, ChIP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human H2BC4 acK11.

대체 명칭 보기

H2BFL, HIST1H2BC, H2BC6, H2BFH, HIST1H2BE, H2BC7, H2BFG, HIST1H2BF, H2BC8, H2BFA, HIST1H2BG, H2BC10, H2BFK, HIST1H2BI, H2BC4, Histone H2B type 1-C/E/F/G/I, Histone H2B.1 A, Histone H2B.a, Histone H2B.g, Histone H2B.h, Histone H2B.k, Histone H2B.l, H2B/a, H2B/g, H2B/h, H2B/k, H2B/l, H2BK11ac

2 이미지

Supplier Data

Immunocytochemistry - Anti-Histone H2B (acetyl K11) antibody (AB240613)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells (treated with 30 mM sodium butyrate for 4 hours) stained for Histone H2B (acetyl K11) using ab240613 at 1/20 dilution in ICC.

The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30 minutes at room temperature. Then primary antibody (1% BSA) was incubated at 4°C overnight.

The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

ChIP - Anti-Histone H2B (acetyl K11) antibody (AB240613)

ChIP

Supplier Data

ChIP - Anti-Histone H2B (acetyl K11) antibody (AB240613)

Hela (human epithelial cell line from cervix adenocarcinoma; 10⁶, treated with 30 mM sodium butyrate for 4 hours) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5 μg ab240613 or a control normal rabbit IgG.

The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ChIP, WB, ICC

applications

Immunogen

Synthetic Peptide within Human H2BC4 acK11. The exact immunogen used to generate this antibody is proprietary information.

P62807

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/20", "ICC-species-notes": "", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "Use 5 μg per reaction.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.. Has broad antibacterial activity. May contribute to the formation of the functional antimicrobial barrier of the colonic epithelium, and to the bactericidal activity of amniotic fluid.

See full target information H2BC4 acK11

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nucleic acids research 50:6190-6210 PubMed35687128

2022

Inhibition of histone acetyltransferase GCN5 by a transcription factor FgPacC controls fungal adaption to host-derived iron stress.

Applications

Unspecified application

Species

Unspecified reactive species

Qin Gu,Yujie Wang,Xiaozhen Zhao,Bingqin Yuan,Mengxuan Zhang,Zheng Tan,Xinyue Zhang,Yun Chen,Huijun Wu,Yuming Luo,Nancy P Keller,Xuewen Gao,Zhonghua Ma

Cell metabolism 32:420-436.e12 PubMed32768387

2020

Sirt2 Inhibition Enhances Metabolic Fitness and Effector Functions of Tumor-Reactive T Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Imene Hamaidi,Lin Zhang,Nayoung Kim,Min-Hsuan Wang,Cristina Iclozan,Bin Fang,Min Liu,John M Koomen,Anders E Berglund,Sean J Yoder,Jiqiang Yao,Robert W Engelman,Ben C Creelan,Jose R Conejo-Garcia,Scott J Antonia,James J Mulé,Sungjune Kim

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H2B (acetyl K11) antibody

Immunocytochemistry - Anti-Histone H2B (acetyl K11) antibody (AB240613)

ChIP - Anti-Histone H2B (acetyl K11) antibody (AB240613)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise