Rabbit Polyclonal H2B butyryl K5 antibody. Suitable for IP, ChIP, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human H2BC4 buK5.
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H2BFL, HIST1H2BC, H2BC6, H2BFH, HIST1H2BE, H2BC7, H2BFG, HIST1H2BF, H2BC8, H2BFA, HIST1H2BG, H2BC10, H2BFK, HIST1H2BI, H2BC4, Histone H2B type 1-C/E/F/G/I, Histone H2B.1 A, Histone H2B.a, Histone H2B.g, Histone H2B.h, Histone H2B.k, Histone H2B.l, H2B/a, H2B/g, H2B/h, H2B/k, H2B/l, H2BK5bu
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (butyryl K5) antibody (AB235429)
HeLa (human epithelial cell line from cervix adenocarcinoma) cells (treated with 30 mM sodium butyrate for 4 hours) labeling Histone H2B (butyryl K5) with ab235429 at 1/15 dilution in ICC/IF.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa-Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L).
- ChIP
Supplier Data
ChIP - Anti-Histone H2B (butyryl K5) antibody (AB235429)
HeLa (human epithelial cell line from cervix adenocarcinoma) (106, treated with 30 mM sodium butyrate for 4 hours) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5 μg ab235429 or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
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제형
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제품이 사용된 논문 (1)
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Molecules and cells 46:351-359 PubMed36921992
2023
Applications
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Species
Unspecified reactive species
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