AB214808

Anti-Histone H3 (acetyl K64) antibody [EPR20713] - ChIP Grade

RabMAb
Advanced Validation
Recombinant
각 태그의 뜻

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(6 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal H3 acetyl K64 antibody. Suitable for ChIP, WB, PepArr, ICC/IF, ChIP-seq and reacts with Human, Mouse, Rat samples. Cited in 6 publications.

대체 명칭 보기

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, HIST1H3C, H3FC, H3C1, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K64ac

6 이미지

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K64) antibody [EPR20713] - ChIP Grade (AB214808)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K64) antibody [EPR20713] - ChIP Grade (AB214808)

Immunofluorescent analysis of 100% methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells untreated and treated with Trichostatin A (500 ng/ml) for 4 hours labeling Histone H3 (acetyl K64) with ab214808 at 1/2000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on HeLa cells. The expression increased after treatment with Trichostatin A (500 ng/ml) for 4 hours.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

ChIP-sequencing - Anti-Histone H3 (acetyl K64) antibody [EPR20713] - ChIP Grade (AB214808)

ChIP-seq

Unknown

ChIP-sequencing - Anti-Histone H3 (acetyl K64) antibody [EPR20713] - ChIP Grade (AB214808)

Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10^7 HeLa cells and 4 μg of ab214808. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads.

Additional screenshots of mapped reads can be downloaded here.

ChIP - Anti-Histone H3 (acetyl K64) antibody [EPR20713] - ChIP Grade (AB214808)

ChIP

Unknown

ChIP - Anti-Histone H3 (acetyl K64) antibody [EPR20713] - ChIP Grade (AB214808)

Chromatin was prepared from HeLa (human epithelial cell line from cervix adenocarcinoma) cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25μg of chromatin 2μg of ab214808(red) and 20μl of Anti rabbit IgG sepharose beads. 2μg of rabbit normal IgG was added to the beads control (grey). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.

Supplier Data

Western blot - Anti-Histone H3 (acetyl K64) antibody [EPR20713] - ChIP Grade (AB214808)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Histone H3 (acetyl K64) antibody [EPR20713] - ChIP Grade (ab214808) at 1/1000 dilution

Lane 1:

Untreated HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

HeLa treated with 400ng/ml Trichostatin A for 9 hours whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 15 kDa

Observed band size: 15 kDa

true

Exposure time: 15s

Supplier Data

Western blot - Anti-Histone H3 (acetyl K64) antibody [EPR20713] - ChIP Grade (AB214808)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Histone H3 (acetyl K64) antibody [EPR20713] - ChIP Grade (ab214808) at 1/5000 dilution

Lane 1:

Untreated NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate at 20 µg

Lane 2:

NIH/3T3 treated with 500ng/ml Trichostatin A for 4 hours whole cell lysate at 20 µg

Lane 3:

Untreated C6 (rat glial tumor cell line) whole cell lysate at 20 µg

Lane 4:

C6 treated with 500ng/ml Trichostatin A for 4 hours whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 15 kDa

Observed band size: 15 kDa

true

Exposure time: 3min

Peptide Array - Anti-Histone H3 (acetyl K64) antibody [EPR20713] - ChIP Grade (AB214808)

PepArr

Supplier Data

Peptide Array - Anti-Histone H3 (acetyl K64) antibody [EPR20713] - ChIP Grade (AB214808)

ab214808 was tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).

Circle area represents affinity between the antibody and a peptide : all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.

The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, is available under the Product Protocol section.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20713

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ChIP-seq, PepArr, ICC/IF, WB, ChIP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "PepArr" : {"fullname" : "Peptide Array", "shortname":"PepArr"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "ChIPseq" : {"fullname" : "ChIP-sequencing", "shortname":"ChIP-seq"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "2 µg for 25 µg chromatin", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "PepArr-species-checked": "testedAndGuaranteed", "PepArr-species-dilution-info": "0.1 µg/mL", "PepArr-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/2000", "ICCIF-species-notes": "", "ChIPseq-species-checked": "testedAndGuaranteed", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "" }, "Mouse": { "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "PepArr-species-checked": "guaranteed", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIPseq-species-checked": "guaranteed", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "" }, "Rat": { "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "PepArr-species-checked": "guaranteed", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIPseq-species-checked": "guaranteed", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "" }, "Arabidopsis thaliana": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIPseq-species-checked": "predicted", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "" }, "Camel": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIPseq-species-checked": "predicted", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "" }, "Dasypus novemcinctus": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIPseq-species-checked": "predicted", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "" }, "Drosophila melanogaster": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIPseq-species-checked": "predicted", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "" }, "Goat": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIPseq-species-checked": "predicted", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "" }, "Northern tree shrew": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIPseq-species-checked": "predicted", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "" }, "Primates": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIPseq-species-checked": "predicted", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "" }, "Pufferfish": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIPseq-species-checked": "predicted", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "" }, "Xenopus tropicalis": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIPseq-species-checked": "predicted", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "" }, "Zebrafish": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIPseq-species-checked": "predicted", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting
Download chipSequencingBookletWebsite|en
Download peptideArrayWebsite|en

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타겟 정보

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

See full target information H3C1 acetyl K64

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제품이 사용된 논문 (6)

Recent publications for all applications. Explore the 전체 목록 and refine your search

PLoS biology 23:e3003239 PubMed40638571

2025

H3K4me2 distinguishes a distinct class of enhancers during the maternal-to-zygotic transition.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew D Hurton,Jennifer M Miller,Miler T Lee

Molecular and cellular endocrinology 586:112194 PubMed38395189

2024

Deficiency of nucleosome-destabilizing factor GLYR1 dampens spermatogenesis in mice.

Applications

ICC

Species

Human

Gui-Ping Cheng,Yu-Fan Wang,Yuan-Yuan Li,Shi-Meng Guo,Hong-Gang Li,Dong-Mei Ji,Nian-Hua Yi,Li-Quan Zhou

Journal of nanobiotechnology 21:280 PubMed37598147

2023

Targeting macrophage M1 polarization suppression through PCAF inhibition alleviates autoimmune arthritis via synergistic NF-κB and H3K9Ac blockade.

Applications

Species

Unspecified reactive species

Jinteng Li,Feng Ye,Xiaojun Xu,Peitao Xu,Peng Wang,Guan Zheng,Guiwen Ye,Wenhui Yu,Zepeng Su,Jiajie Lin,Yunshu Che,Zhidong Liu,Pei Feng,Qian Cao,Dateng Li,Zhongyu Xie,Yanfeng Wu,Huiyong Shen

BMC biology 20:22 PubMed35057804

2022

Histone acetyltransferase NAA40 modulates acetyl-CoA levels and lipid synthesis.

Applications

Species

Mouse

Evelina Charidemou,Maria A Tsiarli,Andria Theophanous,Vural Yilmaz,Chrysoula Pitsouli,Katerina Strati,Julian L Griffin,Antonis Kirmizis

Epigenetics & chromatin 15:2 PubMed34991687

2022

Chromatin alterations during the epididymal maturation of mouse sperm refine the paternally inherited epigenome.

Applications

Species

Mouse

Yudhishtar S Bedi,Alexis N Roach,Kara N Thomas,Nicole A Mehta,Michael C Golding

Autophagy :1-17 PubMed32397873

2020

Involvement of CASP9 (caspase 9) in IGF2R/CI-MPR endosomal transport.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Han,Leslie A Goldstein,Wen Hou,Simon C Watkins,Hannah Rabinowich

제품이 사용된 논문 모두 보기

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chipSequencingBookletWebsite

peptideArrayWebsite

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H3 (acetyl K64) antibody [EPR20713] - ChIP Grade

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K64) antibody [EPR20713] - ChIP Grade (AB214808)

ChIP-sequencing - Anti-Histone H3 (acetyl K64) antibody [EPR20713] - ChIP Grade (AB214808)

ChIP - Anti-Histone H3 (acetyl K64) antibody [EPR20713] - ChIP Grade (AB214808)

Western blot - Anti-Histone H3 (acetyl K64) antibody [EPR20713] - ChIP Grade (AB214808)

Western blot - Anti-Histone H3 (acetyl K64) antibody [EPR20713] - ChIP Grade (AB214808)

Peptide Array - Anti-Histone H3 (acetyl K64) antibody [EPR20713] - ChIP Grade (AB214808)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Sample Prep & Detection Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

제품이 사용된 논문 (6)

Product promise