AB214731

Anti-Histone H3 (acetyl K79) antibody [RM156]

Recombinant
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(2 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal H3 acetyl K79 antibody. Suitable for ChIP, ELISA, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human H3C1 acetyl K79.

대체 명칭 보기

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, HIST1H3C, H3FC, H3C1, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K79ac

5 이미지

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K79) antibody [RM156] (AB214731)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K79) antibody [RM156] (AB214731)

Immunofluorescent analysis of HeLa cells treated with sodium butyrat labeling Histone H3 (Acetyl-Lys79)` `with ab214731 at 2 μg/mL dilution (red); actin filaments have been labeled with fluorescein phalloidin (green).

ChIP - Anti-Histone H3 (acetyl K79) antibody [RM156] (AB214731)

ChIP

Supplier Data

ChIP - Anti-Histone H3 (acetyl K79) antibody [RM156] (AB214731)

ChIP performed on HeLa cells with or without Sodium Butyrate treatment, using ab214731 at 5μg/mL dilution. Real-time PCR was performed using primers specific to the gene ``indicated.

ELISA - Anti-Histone H3 (acetyl K79) antibody [RM156] (AB214731)

ELISA

Supplier Data

ELISA - Anti-Histone H3 (acetyl K79) antibody [RM156] (AB214731)

Sandwich ELISA labeling acetylated Histone H3 at Lys 79 in HeLa whole cell lysate, treated or untreated with Sodium Butyrate using ab214731 at 5 μg/mL dilution as the capture antibody and biotinylated anti-H3CT at 1 μg/mL dilution as the detection antibody.

ELISA

Supplier Data

ELISA - Anti-Histone H3 (acetyl K79) antibody [RM156] (AB214731)

ab214731 specifically reacts to Histone H3 acetylated at Lysine 79 (K79ac). No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56 ``(K56ac), or Lysine 122 (K122) in Histone H3.

Supplier Data

Western blot - Anti-Histone H3 (acetyl K79) antibody [RM156] (AB214731)

All lanes:

Western blot - Anti-Histone H3 (acetyl K79) antibody [RM156] (ab214731) at 1 µg/mL

Lane 1:

acid extracts from HeLa cells untreated

Lane 2:

acid extracts from HeLa cells treated with sodium butyrate

Predicted band size: 15 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

RM156

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ELISA, ICC/IF, ChIP

applications

Immunogen

Synthetic Peptide within Human H3C1 acetyl K79. The exact immunogen used to generate this antibody is proprietary information.

P68431

Specificity

ab214731 reacts to Histone H3 acetylated at Lysine 79 (K79ac). No cross reactivity with other acetylated Lysines in Histone H3.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "2-10 µg/mL", "ChIP-species-notes": "", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "0.2-1 µg/mL", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.5-2 µg/mL", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

See full target information H3C1 acetyl K79

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of nanobiotechnology 21:280 PubMed37598147

2023

Targeting macrophage M1 polarization suppression through PCAF inhibition alleviates autoimmune arthritis via synergistic NF-κB and H3K9Ac blockade.

Applications

Unspecified application

Species

Unspecified reactive species

Jinteng Li,Feng Ye,Xiaojun Xu,Peitao Xu,Peng Wang,Guan Zheng,Guiwen Ye,Wenhui Yu,Zepeng Su,Jiajie Lin,Yunshu Che,Zhidong Liu,Pei Feng,Qian Cao,Dateng Li,Zhongyu Xie,Yanfeng Wu,Huiyong Shen

Disease markers 2021:1571421 PubMed34840626

2021

Characterization of Cancer Stem Cell Characteristics and Development of a Prognostic Stemness Index Cell-Related Signature in Oral Squamous Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jin Feng,Yunxia Li,Ning Wen

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H3 (acetyl K79) antibody [RM156]

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K79) antibody [RM156] (AB214731)

ChIP - Anti-Histone H3 (acetyl K79) antibody [RM156] (AB214731)

ELISA - Anti-Histone H3 (acetyl K79) antibody [RM156] (AB214731)

ELISA - Anti-Histone H3 (acetyl K79) antibody [RM156] (AB214731)

Western blot - Anti-Histone H3 (acetyl K79) antibody [RM156] (AB214731)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise