AB218553

Anti-Histone H3 (acetyl K9) antibody [RM161]

Recombinant
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Rabbit Recombinant Monoclonal H3 acetyl K9 antibody. Suitable for ICC, ChIP, ELISA, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human H3C1 acetyl K9.

대체 명칭 보기

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3C1, H3FC, HIST1H3C, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K9ac

6 이미지

Supplier Data

Immunocytochemistry - Anti-Histone H3 (acetyl K9) antibody [RM161] (AB218553)

Immunocytochemical analysis of HepG2 cells labeling Histone H3 (acetyl K9) using ab218553 at 1 μg/mL.

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K9) antibody [RM161] (AB218553)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K9) antibody [RM161] (AB218553)

Immunocytochemistry analysis of HeLa cells treated with sodium butyrate labeling Histone H3 (acetyl K9) using ab218553 at 2 μg/mL (red). Actin filaments have been labeled with fluorescein phalloidin (green).

ELISA - Anti-Histone H3 (acetyl K9) antibody [RM161] (AB218553)

ELISA

Supplier Data

ELISA - Anti-Histone H3 (acetyl K9) antibody [RM161] (AB218553)

Sandwich ELISA against acetylated histone H3 at Lys 9 using HeLa whole cell lysate, treated or untreated with Sodium Butyrate. Using ab218553 at 1 ug/mL as the capture antibody and biotinylated anti-H3K9ac as the detection antibody.

ChIP - Anti-Histone H3 (acetyl K9) antibody [RM161] (AB218553)

ChIP

Supplier Data

ChIP - Anti-Histone H3 (acetyl K9) antibody [RM161] (AB218553)

ChIP performed on HeLa cells using ab218553 at 5ug. Real-time PCR was performed using primers specific to the gene indicated.

Supplier Data

Western blot - Anti-Histone H3 (acetyl K9) antibody [RM161] (AB218553)

All lanes:

Western blot - Anti-Histone H3 (acetyl K9) antibody [RM161] (ab218553) at 0.25 µg/mL

Lane 1:

HeLa cells untreated

Lane 2:

HeLa cells treated with sodium butyrate

Predicted band size: 15 kDa

false

ELISA

Supplier Data

ELISA - Anti-Histone H3 (acetyl K9) antibody [RM161] (AB218553)

ab218553 specifically reacts to Histone H3 acetylated at Lysine 9 (K9ac). No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56 (K56ac), Lysine 79 (K79ac), or Lysine 122 (K122) in histone H3.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

RM161

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB, ChIP, ICC, ELISA

applications

Immunogen

Synthetic Peptide within Human H3C1 acetyl K9. The exact immunogen used to generate this antibody is proprietary information.

P68431

Specificity

Reacts to Histone H3 (acetyl K9). No cross reactivity with other acetylated Lysines in histone H3.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "0.5-2 µg/mL", "ICC-species-notes": "", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "2-10 µg/mL", "ChIP-species-notes": "", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "0.2-1 µg/mL", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.25-1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.5-2 µg/mL", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

See full target information H3C1 acetyl K9

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of virology 99:e0198024 PubMed39976465

2025

Zika virus inhibits cell death by inhibiting the expression of NLRP3 and A20.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Li,Changyang Zhu,Yang Meng,Linliang Zhang,Cong Liu,Yali Qin,Mingzhou Chen

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H3 (acetyl K9) antibody [RM161]

Immunocytochemistry - Anti-Histone H3 (acetyl K9) antibody [RM161] (AB218553)

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K9) antibody [RM161] (AB218553)

ELISA - Anti-Histone H3 (acetyl K9) antibody [RM161] (AB218553)

ChIP - Anti-Histone H3 (acetyl K9) antibody [RM161] (AB218553)

Western blot - Anti-Histone H3 (acetyl K9) antibody [RM161] (AB218553)

ELISA - Anti-Histone H3 (acetyl K9) antibody [RM161] (AB218553)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise