Anti-Histone H3 (acetyl K9 + K14 + K18 + K23 + K27) antibody

• ChIP

Supplier Data

ChIP - Anti-Histone H3 (acetyl K9 + K14 + K18 + K23 + K27) antibody (AB47915)

ChIP was performed using a high sensitivity kit with 5 μg of chromatin from HeLa (Human epithelial cell line from cervix adenocarcinoma) cells and 10 μL of Anti-Histone H3 (acetyl K9 + K14 + K18 + K23 + K27) antibody ab47915.

ChIP DNA was used in qPCR with the negative control primer pairs or gene-specific primer pairs as indicated. Data are presented as binding events detected per 1000 cells using a normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.

• ChIP

PubMed

ChIP - Anti-Histone H3 (acetyl K9 + K14 + K18 + K23 + K27) antibody (AB47915)

HP1α delocalises from chromocentres upon proteasome inhibition whereas the histone modifications remain unaffected.

(A) Proteasome inhibition does not affect canonical histone modifications on major and minor satellite repeats. NIH/3T3 (Mouse embryo fibroblast cell line) cells were treated with 20μM MG132 for 4h followed by ChIP–qPCR analysis using antibodies against repressive mark H3K9me3 and activating marks H3K4me3, H3K36me3, and H3ac (ab47915). Data is shown as relative enrichment to H3 with background subtraction. The y-axis scale was adjusted depending on the signal obtained with different antibodies. Error bars = SEM of 3 biological replicates.

Natisvili et al PLoS One. 2016 Nov 2;11(11):e0165873. doi: 10.1371/journal.pone.0165873. eCollection 2016. Fig 4. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

• Dot

Supplier Data

Dot Blot - Anti-Histone H3 (acetyl K9 + K14 + K18 + K23 + K27) antibody (AB47915)

ab47915 tested by dot blot analysis.Dot blot analysis was used to confirm the specificity of ab47915. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at a dilution of 1 : 1,000. The amount of peptide (picomoles) spotted is indicated next to the row.
Lane 1 : acetyl-Lys9 peptide. Lane 2 : unmodified Lys9 peptide. Lane 3 : acetyl-Lys14 peptide. Lane 4 : unmodified Lys14 peptide. Lane 5 : acetyl-Lys18 peptide. Lane 6 : unmodified Lys18 peptide. Lane 7 : acetyl-Lys23 peptide. Lane 8 : unmodified Lys23 peptide. Lane 9 : acetyl-Lys27 peptide. Lane 10 : unmodified Lys27 peptide.