AB219407

Anti-Histone H3 (citrulline R17) antibody [EPR20358-120]

Name: Anti-Histone H3 (citrulline R17) antibody [EPR20358-120]
Brand: Abcam Limited
SKU: AB219407
Rating: 5 (1 reviews)

RabMAb
Recombinant
20ul selling size
각 태그의 뜻

(1 리뷰)

(26 제품이 사용된 논문 )

Anti-Histone H3 (citrulline R17) antibody [EPR20358-120] (ab219407) is a rabbit monoclonal antibody detecting Histone H3 in Western Blot, Flow Cytometry (Intra), IP, ICC/IF, ELISA. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications

8 이미지

Flow Cytometry (Intracellular) - Anti-Histone H3 (citrulline R17) antibody [EPR20358-120] (AB219407)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Histone H3 (citrulline R17) antibody [EPR20358-120] (AB219407)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HEK-293T (Human epithelial cell line from embryonic kidney) transfected with empty vector (left panel) or PADI4 (WT, right panel), then treated with 10mM CaCl2 and 10μM Ionomycin (ab120116) for 2 hours, labeling Histone H3 (citrulline R17)with ab219407 at 1/500 dilution, followed by Goat anti rabbit IgG (Alexa Fluor^® 647) at 1/2000 dilution.

Positive signal is obtained from HEK-293T cells transfected with WT PADI4 treated with 10mM CaCl2 and 10μM Ionomycin (ab120116) for 2 hours.

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (citrulline R17) antibody [EPR20358-120] (AB219407)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (citrulline R17) antibody [EPR20358-120] (AB219407)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (Human epithelial cell line from embryonic kidney) cells transfected with GFP only or a GFP-tagged PADI4 expression construct, then treated with 10mM CaCl₂ for 2 hours, labeling Histone H3 (citrulline R17) with ab219407 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing positive staining on HEK-293T cells transfected with a GFP-tagged PADI4 expression construct, then treated with 10mM CaCl₂ for 2 hours.

The nuclear counterstain is DAPI (blue).

Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594)) at 1/200 dilution (red).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

Lab

Immunoprecipitation - Anti-Histone H3 (citrulline R17) antibody [EPR20358-120] (AB219407)

Histone H3 (citrulline R17) was immunoprecipitated from 0.35 mg of HEK-293T (Human epithelial cell line from embryonic kidney) transfected with PADI4 (WT), then treated with 10mM CaCl₂ and 10μM Ionomycin (ab120116) for 2 hours, whole cell lysate with ab219407 at 1/30 dilution.

Western blot was performed from the immunoprecipitate using ab219407 at 1/1000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : HEK-293T transfected with PADI4 (WT), then treated with 10mM CaCl₂ and 10μM Ionomycin (ab120116) for 2 hours, whole cell lysate 10 μg (Input).
Lane 2 : ab219407 IP in HEK-293T transfected with PADI4 (WT), then treated with 10mM CaCl₂ and 10μM Ionomycin (ab120116) for 2 hours, whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab219407 in HEK-293T transfected with PADI4 (WT), then treated with 10mM CaCl₂ and 10μM Ionomycin (ab120116) for 2 hours, whole cell lysate.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Histone H3 (citrulline R17) antibody [EPR20358-120] (ab219407)

Predicted band size: 15 kDa

false

Exposure time: 1s

Indirect ELISA - Anti-Histone H3 (citrulline R17) antibody [EPR20358-120] (AB219407)

I-ELISA

Supplier Data

Indirect ELISA - Anti-Histone H3 (citrulline R17) antibody [EPR20358-120] (AB219407)

ELISA analysis of Human Histone H3 (citrulline R17) recombinant protein at 100 ng/ml with ab219407. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.

Supplier Data

Western blot - Anti-Histone H3 (citrulline R17) antibody [EPR20358-120] (AB219407)

Blocking/Dilution buffer : 5% BSA/TBST.

Exposure time : Lane 1 : 1 second; Lane 2 : 3 minutes.

Lane 1:

Western blot - Anti-Histone H3 (citrulline R17) antibody [EPR20358-120] (ab219407) at 1/1000 dilution

Lane 2:

Western blot - Anti-Histone H3 (citrulline R17) antibody [EPR20358-120] (ab219407) at 1/200 dilution

Lane 1:

E12 mouse embryo lysate at 10 µg

Lane 2:

E12 rat embryo lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 15 kDa

Observed band size: 15 kDa

false

ELISA - Anti-Histone H3 (citrulline R17) antibody [EPR20358-120] (AB219407)

ELISA

Unknown

ELISA - Anti-Histone H3 (citrulline R17) antibody [EPR20358-120] (AB219407)

Direct ELISA using ab219407 at 0-1000 ng/ml, followed by Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at 1/2500 dilution.

Antigen concentration : 1000 ng/ml.

Lab

Western blot - Anti-Histone H3 (citrulline R17) antibody [EPR20358-120] (AB219407)

Blocking/Dilution buffer : 5% BSA/TBST.

Histone H3R17 is citrullinated by PADI4 and CaCl₂ is used as a cofactor according to the literature (PMID : 16567635). Ionomycin is used to improve the modification by PADI4 according to the literature (PMID : 26360112).

All lanes:

Western blot - Anti-Histone H3 (citrulline R17) antibody [EPR20358-120] (ab219407) at 1/5000 dilution

Lane 1:

HEK-293T (Human epithelial cell line from embryonic kidney) transfected with empty vector with GFP tag (vector control), then treated with 10mM CaCl2 and 10µM Ionomycin (<a href='/ko/products/biochemicals/ionomycin-ca2-salt-ca2-ionophore-ab120116'>ab120116</a>) for 2 hour at 10 µg

Lane 2:

HEK-293T (Human epithelial cell line from embryonic kidney) transfected with PADI4 (WT), then treated with 10mM CaCl2 and 10µM Ionomycin (<a href='/ko/products/biochemicals/ionomycin-ca2-salt-ca2-ionophore-ab120116'>ab120116</a>) for 2 hours, whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 15 kDa

Observed band size: 15 kDa

false

Exposure time: 3s

Lab

Western blot - Anti-Histone H3 (citrulline R17) antibody [EPR20358-120] (AB219407)

Blocking/Dilution buffer : 5% BSA/TBST.

All lanes:

Western blot - Anti-Histone H3 (citrulline R17) antibody [EPR20358-120] (ab219407) at 1/5000 dilution

Lane 1:

NIH/3T3 (Mouse embryonic fibroblast cell line) transfected with empty vector with GFP tag (vector control), then treated with 10mM CaCl2 for 2 hours, whole cell lysate at 10 µg

Lane 2:

NIH/3T3 (Mouse embryonic fibroblast cell line) transfected with PADI4 (WT) then treated with 10mM CaCl2 for 2 hours, whole cell lysate at 10 µg

Lane 3:

C6 (Rat glial tumor cell line) transfected with empty vector with GFP tag (vector control) then treated with 10mM CaCl2 and 10µM Ionomycin (<a href='/ko/products/biochemicals/ionomycin-ca2-salt-ca2-ionophore-ab120116'>ab120116</a>) for 2 hours, whole cell lysate at 10 µg

Lane 4:

C6 (Rat glial tumor cell line) transfected with PADI4 (WT), then treated with 10mM CaCl2 and 10µM Ionomycin (<a href='/ko/products/biochemicals/ionomycin-ca2-salt-ca2-ionophore-ab120116'>ab120116</a>) for 2 hours, whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 15 kDa

Observed band size: 15 kDa

false

Exposure time: 1s

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20358-120

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IP, WB, ICC/IF, Flow Cyt (Intra), ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Treatment is required for detection of the target protein. See Protocol section for detailed information. 更多详细信息请参阅 Protocol 部分.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Recombinant fragment - Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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제품 세부 정보

What is this antibody validated in?
Anti-Histone H3 (citrulline R17) antibody [EPR20358-120] (ab219407) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Immunoprecipitation (IP), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA in Human, Mouse, Rat samples.

What is the molecular weight of Histone H3?
Anti-Histone H3 (citrulline R17) [EPR20358-120] (ab219407) specifically detects a band for Histone H3 (UniProt: P68431) at a molecular weight of 15kDa.

Trusted by the scientific community
Anti-Histone H3 (citrulline R17) [EPR20358-120] (ab219407) was first used in a scientific publication in 2017 and has been cited over 10 times in peer-reviewed journals.

Other related products
We have a range of other formats of antibody clone [EPR20358-120] also available for your convenience: ab219407, Carrier free - ab232938, Alexa Fluor® 647 - ab300305

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 20µl. Discover our selection of trial-size antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

Treatment Conditions:

Chemical treatment is required for detection of endogenous citrullinated proteins in cell lines. PADs (including PADI2 and PADI4) are the enzymes that hydrolyze arginine to citrulline, in the presence of Ca²⁺ and ionomycin. Please refer to PMID: 26360112

Controls:
- Mock (no transfection)
- Transfection reagent–only control
- Empty vector control
- Vehicle control for chemical treatment

Generation of PADI2 or PADI4 overexpression cell line and treatment with CaCl₂/Ionomycin:

1. Prior to transfection with PADI2 or PADI4 expression vector, seed cells in complete medium and place in a 37°C, 5% CO₂ incubator for 24 hours or until cells reach 70-80% confluence.

For a single T-175 flask we recommend seeding the following common cell lines as follows in 30 mL media:
- HEK-293T: 1x10⁷ cells
- MCF7: 2.5-4x10⁶ cells
- NIH3T3: 4-5x10⁶ cells
- C6: 3-4x10⁶ cells

2. Once cells have reached confluence, prepare the PADI2 or PADI4 expression vector for transfection using your preferred method (PMID: 33976969).
3. 24-48 hours after transfection, remove the medium from the cells and replace with fresh complete medium containing 10 mM CaCl₂ (ab285384) and 10 uM Ionomycin (ab120116) and incubate for 2-4 hours.

Key Considerations:
For alternative cell lines, you should confirm the transfectability, growth rate, and sensitivity of the cell line to the planned chemical treatment. Incubation times and treatment length may need to be adjusted.

Western Blot Conditions:
Recommended SDS-PAGE Gel Type: 4-20% Tris-glycine
Recommended Running Buffer: Tris-Glycine-SDS
Primary: 1/1000-1/5000 dilution at 4°C overnight or room temperature for 1 hour
Blocking: 5% BSA at room temperature for 1 hour

Positive Control: Cell line overexpressing PADI4 and treated with CaCl₂/Ionomycin
Negative Control: Cell line overexpressing PADI4

Additional resources:
Lysate preparation protocol
Lysate preparation protocol - nuclear proteins
Lysate preparation protocol - low molecular weight proteins
Western blot troubleshooting

ICC Conditions:
Primary: 1/100 dilution at 4°C overnight or room temperature for 2 hours
Fixation: 4% PFA at room temperature for 10-20 minutes
Permeabilization: 0.1 % Triton X-100 at room temperature for 2-5 minutes
Blocking: 2-10% serum from secondary host at room temperature for 1-2 hours

Positive Control: Cell line overexpressing PADI4 and treated with CaCl2/Ionomycin
Negative Control: Cell line overexpressing PADI4
Secondary only control:

Additional resources:
ICC Protocol

Flow Cytometry Conditions:
Primary: 1/500 dilution at 4°C for 30 minutes
Fixation: 4% PFA at room temperature for 10 minutes
Permeabilization:

Positive Control: Cell line overexpressing PADI4 and treated with CaCl2/Ionomycin
Negative Control: Cell line overexpressing PADI4
Secondary only control:

Additional resources:
Flow Cytometry protocol
Flow cytometry troubleshooting

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타겟 정보

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

See full target information H3C1 citrulline R17

대체 명칭 보기

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3FC, HIST1H3C, H3C1, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3R17cit

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제품이 사용된 논문 (26)

Recent publications for all applications. Explore the 전체 목록and refine your search

Molecular and cellular biochemistry 480:6225-6238 PubMed40859095

2025

NETs accelerate aortic valve calcification by promoting M1 macrophage polarization through the TLR9 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Luyao Liu,Zhenzhen Lan,Xincan Liu,Yun Chen,Zhihao Chen,Li Cheng,Ting Hu

Nature 646:462-473 PubMed40836096

2025

Cancer-induced nerve injury promotes resistance to anti-PD-1 therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Erez N Baruch,Frederico O Gleber-Netto,Priyadharsini Nagarajan,Xiayu Rao,Shamima Akhter,Tuany Eichwald,Tongxin Xie,Mohammad Balood,Adebayo Adewale,Shorook Naara,Hinduja N Sathishkumar,Shajedul Islam,William McCarthy,Brandi J Mattson,Renata Ferrarotto,Michael K Wong,Michael A Davies,Sonali Jindal,Sreyashi Basu,Karine Roversi,Amin Reza Nikpoor,Maryam Ahmadi,Ali Ahmadi,Catherine Harwood,Irene Leigh,Dennis Gong,Paulino Tallón de Lara,Derrick L Tao,Tara M Davidson,Nadim J Ajami,Andrew Futreal,Kunal Rai,Veena Kochat,Micah Castillo,Preethi Gunaratne,Ryan P Goepfert,Sharia D Hernandez,Nikhil I Khushalani,Jing Wang,Stephanie S Watowich,George A Calin,Michael R Migden,Mona Yuan,Naijiang Liu,Yi Ye,William L Hwang,Paola D Vermeer,Nisha J D'Silva,Yuri L Bunimovich,Dan Yaniv,Jared K Burks,Javier Gomez,Patrick M Dougherty,Kenneth Y Tsai,James P Allison,Padmanee Sharma,Jennifer A Wargo,Jeffrey N Myers,Sebastien Talbot,Neil D Gross,Moran Amit

Renal failure 47:2545983 PubMed40796802

2025

Suppression of nF-κB by ro 106-9920 alleviates ischemia/reperfusion-induced renal dysfunction and inflammation via modulation of neutrophil extracellular trap formation in acute kidney injury mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Wang,Feng Gao

World journal of gastroenterology 31:106166 PubMed40741100

2025

Myeloperoxidase, extracellular DNA and neutrophil extracellular trap formation in the animal models of metabolic dysfunction-associated steatotic liver disease.

Applications

Unspecified application

Species

Unspecified reactive species

Andrej Feješ,Paulína Belvončíková,Emil Bečka,Tomáš Strečanský,Michal Pastorek,Jakub Janko,Barbora Filová,Pavel Babál,Katarína Šebeková,Veronika Borbélyová,Roman Gardlík

Nature communications 16:5393 PubMed40562767

2025

PADI4-mediated citrullination of histone H3 stimulates HIV-1 transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Luca Love,Bianca B Jütte,Birgitta Lindqvist,Hannah Rohdjess,Oscar Kieri,Piotr Nowak,J Peter Svensson

American journal of translational research 17:3898-3907 PubMed40535640

2025

Dectin-1 participates in β-glucan- or PMA-induced neutrophil extracellular trap formation during antifungal defense.

Applications

Unspecified application

Species

Unspecified reactive species

Shoude Zhang,Ying Lu,Yuan Zhao,Zhanwei Dong,Mao Jin,Mina Xu,Hong Pan,Mang Xiao

Biomolecules 15: PubMed40305335

2025

Metabolic Implications of Elevated Neutrophil Extracellular Traps in Polycystic Ovary Syndrome: A Focus on Hepatic Glycolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Siyu Lin,Yushan Li,Wei Liu,Yanzhi Du,Tao Tao

International journal of molecular sciences 26: PubMed40076519

2025

Therapeutic Effects of DNase I on Peripheral and Local Markers of Liver Injury and Neutrophil Extracellular Traps in a Model of Alcohol-Related Liver Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Paulína Belvončíková,Andrej Feješ,Barbora Gromová,Ľubica Janovičová,Anna Farkašová,Pavel Babál,Roman Gardlík

Journal of translational medicine 23:180 PubMed39953550

2025

Integrative multi-omics analysis of radionuclide-induced intestinal injury reveals the radioprotective role of L-citrulline through histone H3-mediated Cxcl3.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Yang,Hao Bai,Tao Yan,Xiaopeng Xu,Xiaoyou Tang,Bin Song,Yulan Liu,Yunyun Lu,Pengfei Liu,Wenling Tu,Yuhong Shi,Shuyu Zhang

Precision clinical medicine 7:pbae028 PubMed39540022

2024

Targeted nuclear degranulation of neutrophils promotes the progression of pneumonia in ulcerative colitis.

Applications

Unspecified application

Species

Unspecified reactive species

Yiming Shao,Qibing Zheng,Xiaobei Zhang,Ping Li,Xingxin Gao,Liming Zhang,Jiahong Xu,Lingchao Meng,Yanyun Tian,Qinqin Zhang,Guangxi Zhou

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H3 (citrulline R17) antibody [EPR20358-120]

Flow Cytometry (Intracellular) - Anti-Histone H3 (citrulline R17) antibody [EPR20358-120] (AB219407)

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (citrulline R17) antibody [EPR20358-120] (AB219407)

Immunoprecipitation - Anti-Histone H3 (citrulline R17) antibody [EPR20358-120] (AB219407)

Indirect ELISA - Anti-Histone H3 (citrulline R17) antibody [EPR20358-120] (AB219407)

Western blot - Anti-Histone H3 (citrulline R17) antibody [EPR20358-120] (AB219407)

ELISA - Anti-Histone H3 (citrulline R17) antibody [EPR20358-120] (AB219407)

Western blot - Anti-Histone H3 (citrulline R17) antibody [EPR20358-120] (AB219407)

Western blot - Anti-Histone H3 (citrulline R17) antibody [EPR20358-120] (AB219407)

관련 conjugated 항체와 다양한 조성의 항체 (2)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (26)

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