AB272142

Anti-Histone H3 (di methyl K4) antibody

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Rabbit Polyclonal H3 di methyl K4 antibody. Suitable for ChIP, Dot, WB, ICC/IF and reacts with Human, Caenorhabditis elegans, Synthetic peptide samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human H3C1 di methyl K4.

대체 명칭 보기

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3C1, H3FC, HIST1H3C, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K4me2, H3K4me, H3K4

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ChIP - Anti-Histone H3 (di methyl K4) antibody (AB272142)

ChIP

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ChIP - Anti-Histone H3 (di methyl K4) antibody (AB272142)

Chromatin Immunoprecipitation of ab272142. Chromatin from one million formaldehyde cross-linked HeLa (Human epithelial cell line from cervix adenocarcinoma) cells was used with 2ug of ab272142 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.

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Western blot - Anti-Histone H3 (di methyl K4) antibody (AB272142)

Block : 5% BLOTTO

All lanes:

Western blot - Anti-Histone H3 (di methyl K4) antibody (ab272142) at 1/500 dilution

All lanes:

HeLa (Human epithelial cell line from cervix adenocarcinoma) histone preparation at 30 µg

Secondary

All lanes:

IRDye800™ rabbit secondary antibody at 1/10000 dilution

Predicted band size: 15 kDa

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Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K4) antibody (AB272142)

ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K4) antibody (AB272142)

Immunofluorescence analysis of Caenorhabditis elegans embryo stained for Histone H3 (green) using ab272142 at 1 : 50, followed by a Dylight 488 secondary antibody at 1 : 10,000. Localization : Histone H3 [Dimethyl Lys4] is nuclear and chromosomal. Counterstaining of nuclei with DAPI (blue). Fixation : 0.5% PFA. Antigen retrieval : Not required.

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Western blot - Anti-Histone H3 (di methyl K4) antibody (AB272142)

Block : 5% BLOTTO

All lanes:

Western blot - Anti-Histone H3 (di methyl K4) antibody (ab272142) at 1/500 dilution

All lanes:

Caenorhabditis elegans embryo lysate at 30 µg

Secondary

All lanes:

IRDye800™ rabbit secondary antibody at 1/10000 dilution

Predicted band size: 15 kDa

false

Unknown

Dot Blot - Anti-Histone H3 (di methyl K4) antibody (AB272142)

Dot Blot analysis of ab272142 at 1 : 1000, followed by Dylight™488 rabbit secondary antibody at 1 : 10,000. Block : 5% BLOTTO.

Lane 1 : K4.

Lane 2 : K4 Kme1.

Lane 3 : K4 Kme2.

Lane 4 : K4 Kme3.

Lane 5 : K4 KAc.

Load : 1, 3, 10, and 25 picomoles of peptide.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Caenorhabditis elegans

Applications

Dot, ICC/IF, ChIP, WB

applications

Immunogen

Synthetic Peptide within Human H3C1 di methyl K4. The exact immunogen used to generate this antibody is proprietary information.

P68431

Specificity

This antibody reacts with human Histone H3.2. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "2-5 µg/cells for 6 µg/cells", "ChIP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Caenorhabditis elegans": { "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Synthetic peptide": { "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

Anti-Histone H3 [Dimethyl Lys4] was affinity purified from monospecific antiserum by immunoaffinity chromatography.

보관 버퍼

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 30% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.424% Potassium phosphate solution

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

See full target information H3C1 di methyl K4

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nucleic acids research 53: PubMed40036504

2025

Argonaute CSR-1A promotes H3K9me3 maintenance to protect somatic development in offspring.

Applications

Unspecified application

Species

Unspecified reactive species

Di Rao,Dengfeng Li,Lili Li,Junchao Xue,Shikui Tu,En-Zhi Shen

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H3 (di methyl K4) antibody

ChIP - Anti-Histone H3 (di methyl K4) antibody (AB272142)

Western blot - Anti-Histone H3 (di methyl K4) antibody (AB272142)

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K4) antibody (AB272142)

Western blot - Anti-Histone H3 (di methyl K4) antibody (AB272142)

Dot Blot - Anti-Histone H3 (di methyl K4) antibody (AB272142)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise