- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (mono methyl K4) antibody [RM140] (AB205256)
Immunocytochemistry/Immunofluorescence analysis of HeLa cells treated with sodium butyrate, labelling Histone H3 (mono methyl K4) with ab205256 at 1 – 2 μg/mL (red). Actin filaments have been labeled with fluorescein phalloidin (green).
- ChIP
Supplier Data
ChIP - Anti-Histone H3 (mono methyl K4) antibody [RM140] (AB205256)
ChIP performed on HeLa cells using ab205256 (5ug). Real-time PCR was performed using primers specific to the gene indicated.
- ELISA
Supplier Data
ELISA - Anti-Histone H3 (mono methyl K4) antibody [RM140] (AB205256)
ab205256 reacts to Histone H3 monomethylated at Lysine 4 (K4me1). No cross reactivity with dimethylated Lysine 4 (K4me2), trimethylated Lysine 4 (K4me3), or other methylations in histone H3.
- WB
Supplier Data
Western blot - Anti-Histone H3 (mono methyl K4) antibody [RM140] (AB205256)
All lanes:
Western blot - Anti-Histone H3 (mono methyl K4) antibody [RM140] (ab205256) at 0.5 µg/mL
Lane 1:
Recombinant histone H3.3
Lane 2:
Acid extracts of HeLa cells
Predicted band size: 15 kDa
false
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CytoJournal 21:45 PubMed39737125
2024
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Investigative ophthalmology & visual science 60:4619-4631 PubMed31675426
2019
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