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AB205256

Anti-Histone H3 (mono methyl K4) antibody [RM140]

5

(1 리뷰)

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(2 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal H3 mono methyl K4 antibody. Suitable for ChIP, ELISA, WB, ICC/IF and reacts with Human, Synthetic peptide samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human H3C1 mono methyl K4.
4 이미지
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (mono methyl K4) antibody [RM140] (AB205256)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (mono methyl K4) antibody [RM140] (AB205256)

Immunocytochemistry/Immunofluorescence analysis of HeLa cells treated with sodium butyrate, labelling Histone H3 (mono methyl K4) with ab205256 at 1 – 2 μg/mL (red). Actin filaments have been labeled with fluorescein phalloidin (green).

ChIP - Anti-Histone H3 (mono methyl K4) antibody [RM140] (AB205256)
  • ChIP

Supplier Data

ChIP - Anti-Histone H3 (mono methyl K4) antibody [RM140] (AB205256)

ChIP performed on HeLa cells using ab205256 (5ug). Real-time PCR was performed using primers specific to the gene indicated.

ELISA - Anti-Histone H3 (mono methyl K4) antibody [RM140] (AB205256)
  • ELISA

Supplier Data

ELISA - Anti-Histone H3 (mono methyl K4) antibody [RM140] (AB205256)

ab205256 reacts to Histone H3 monomethylated at Lysine 4 (K4me1). No cross reactivity with dimethylated Lysine 4 (K4me2), trimethylated Lysine 4 (K4me3), or other methylations in histone H3.

Western blot - Anti-Histone H3 (mono methyl K4) antibody [RM140] (AB205256)
  • WB

Supplier Data

Western blot - Anti-Histone H3 (mono methyl K4) antibody [RM140] (AB205256)

All lanes:

Western blot - Anti-Histone H3 (mono methyl K4) antibody [RM140] (ab205256) at 0.5 µg/mL

Lane 1:

Recombinant histone H3.3

Lane 2:

Acid extracts of HeLa cells

Predicted band size: 15 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

RM140

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, ChIP, ELISA

applications

Immunogen

Synthetic Peptide within Human H3C1 mono methyl K4. The exact immunogen used to generate this antibody is proprietary information.

P68431

Specificity

ab205256 reacts to Histone H3 monomethylated at Lysine 4 (K4me1). No cross reactivity with dimethylated Lysine 4 (K4me2), trimethylated Lysine 4 (K4me3), or other methylations in histone H3.

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "2-10 µg/mL", "ChIP-species-notes": "<p></p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-2 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Synthetic peptide": { "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "0.2-1 µg/mL", "ELISA-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
Purification 관련 사항
ab205256 was purified from an animal origin–free culture supernatant.
보관 버퍼
Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

타겟 정보

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
See full target information H3C1 mono methyl K4

대체 명칭 보기

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3FC, HIST1H3C, H3C1, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K4me, H3K4me1, H3K4

제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록and refine your search

CytoJournal 21:45 PubMed39737125

2024

Knockdown of ribosomal protein L22-like 1 arrests the cell cycle and promotes apoptosis in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Chunming Li,Xinna Du,Hu Zhang,Shuang Liu

Investigative ophthalmology & visual science 60:4619-4631 PubMed31675426

2019

Characterization of LSD1 Expression Within the Murine Eye.

Applications

Unspecified application

Species

Unspecified reactive species

Salma Ferdous,Hans E Grossniklaus,Jeffrey H Boatright,John M Nickerson
제품이 사용된 논문 모두 보기

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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