AB183626

Anti-Histone H3 (phospho S10) antibody

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(5 제품이 사용된 논문 )

Rabbit Polyclonal H3 phospho S10 antibody. Suitable for IP, ChIP, WB, IHC-P, ICC/IF, IHC - Wmt and reacts with Human, Mouse, Rat, Zebrafish samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human H3C1 phospho S10.

10 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (phospho S10) antibody (AB183626)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (phospho S10) antibody (AB183626)

Immunohistochemical analysis of paraffin embedded Human gastric carcinoma tissue labeling Histone H3 (phospho S10) with ab183626 at 1/500.

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (phospho S10) antibody (AB183626)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (phospho S10) antibody (AB183626)

Immunofluorescent analysis of RFP-tag transfected HeLa cells labeling Histone H3 (phospho S10) with ab183626 at 1/500 (green). Red : Tubulin, alpha 1b protein stained by TUBA1B antibody at 1/500 . Blue : Hoechst 33342 staining.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (phospho S10) antibody (AB183626)

Confocal immunofluorescent analysis of mitotic HeLa cells labeling Histone H3 (phospho S10) with ab183626 at 1/500 (green). Red : Tubulin, alpha 1b protein stained by TUBA1B antibody at 1/2500 . Blue : Hoechst 33342 staining.

Supplier Data

Immunoprecipitation - Anti-Histone H3 (phospho S10) antibody (AB183626)

Samples were separated by 15 % SDS-PAGE.

The immunoprecipitated HIST1H3B protein was detected by Histone H3S10ph (phospho Ser10) antibody diluted at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-Histone H3 (phospho S10) antibody (ab183626) at 1/1000 dilution

Lane 1:

293T whole cell extract treat with 100ng/ml Nocodazole for 24 hr at 30 µg

Lane 2:

Control with 4 µg of preimmune Rabbit IgG

Lane 3:

293T whole cell extract treat with 100ng/ml Nocodazole for 24 hr (+)

Secondary

All lanes:

Anti rabbit IgG antibody

Predicted band size: 15 kDa

false

ChIP - Anti-Histone H3 (phospho S10) antibody (AB183626)

ChIP

Supplier Data

ChIP - Anti-Histone H3 (phospho S10) antibody (AB183626)

Cross-linked ChIP was performed with HeLa chromatin extract treated with Nocodazole (100 ng/ml for 24 h) and 5 μg of either control rabbit IgG or anti-Histone H3 (phospho S10) antibody (ab183626). The precipitated DNA was detected by PCR with primer set targeting to GAPDH promoter.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (phospho S10) antibody (AB183626)

Immunohistochemical analysis of paraffin-embedded mouse stomach tissue stained for Histone H3S10ph (phospho Ser10) protein at nucleus with ab183626 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

Supplier Data

Western blot - Anti-Histone H3 (phospho S10) antibody (AB183626)

All lanes:

Western blot - Anti-Histone H3 (phospho S10) antibody (ab183626) at 1/1000 dilution

Lane 1:

U2OS whole cell lysate (untreated) at 30 µg

Lane 2:

U2OS whole cell lysate (serum deprivation for 48hr) at 30 µg

Lane 3:

U2OS whole cell lysate (100ng/ml Nocodazole treatment for 24hr) at 30 µg

Predicted band size: 15 kDa

false

Supplier Data

Western blot - Anti-Histone H3 (phospho S10) antibody (AB183626)

All lanes:

Western blot - Anti-Histone H3 (phospho S10) antibody (ab183626) at 1/1000 dilution

Lane 1:

4T1 whole cell lysate at 30 µg

Lane 2:

4T1 whole cell lysate (100ng/ml Nocodazole treatment for 24hr) at 30 µg

Predicted band size: 15 kDa

false

IHC - Wholemount - Anti-Histone H3 (phospho S10) antibody (AB183626)

IHC - Wmt

Supplier Data

IHC - Wholemount - Anti-Histone H3 (phospho S10) antibody (AB183626)

Immunohistochemistry (wholemount) analysis of zebrafish labelling Histone H3 (phopho S10) with ab183626 at a 1/100 dilution.

Sample : Paraformaldehyde-fixed 2 day-post-fertilization zebrafish embryo.

IHC - Wmt

Supplier Data

IHC - Wholemount - Anti-Histone H3 (phospho S10) antibody (AB183626)

Immunohistochemistry (wholemount) analysis of zebrafish labelling Histone H3 (phopho S10) with ab183626 at a 1/100 dilution.

Sample : Paraformaldehyde-fixed 2 day-post-fertilization zebrafish embryo.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human, Zebrafish

Applications

ChIP, IHC - Wmt, IHC-P, IP, ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human H3C1 phospho S10. The exact immunogen used to generate this antibody is proprietary information.

P68431

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCWmt" : {"fullname" : "IHC - Wholemount", "shortname":"IHC - Wmt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "", "IHCWmt-species-checked": "guaranteed", "IHCWmt-species-dilution-info": "", "IHCWmt-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCWmt-species-checked": "guaranteed", "IHCWmt-species-dilution-info": "", "IHCWmt-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCWmt-species-checked": "guaranteed", "IHCWmt-species-dilution-info": "", "IHCWmt-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCWmt-species-checked": "predicted", "IHCWmt-species-dilution-info": "", "IHCWmt-species-notes": "" }, "Zebrafish": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCWmt-species-checked": "testedAndGuaranteed", "IHCWmt-species-dilution-info": "1/100 - 1/500", "IHCWmt-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

See full target information H3C1 phospho S10

대체 명칭 보기

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3C1, H3FC, HIST1H3C, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3S10p

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제품이 사용된 논문 (5)

Recent publications for all applications. Explore the 전체 목록and refine your search

Developmental cell 58:2802-2818.e5 PubMed37714159

2023

An improved Erk biosensor detects oscillatory Erk dynamics driven by mitotic erasure during early development.

Applications

Unspecified application

Species

Unspecified reactive species

Scott G Wilcockson,Luca Guglielmi,Pablo Araguas Rodriguez,Marc Amoyel,Caroline S Hill

Glia 71:259-283 PubMed36128720

2022

Pten associates with important gene regulatory network to fine-tune Müller glia-mediated zebrafish retina regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Shivangi Gupta,Poonam Sharma,Mansi Chaudhary,Sharanya Premraj,Simran Kaur,Vijithkumar Vijayan,Manas Geeta Arun,Nagaraj Guru Prasad,Rajesh Ramachandran

International journal of molecular medicine 50: PubMed36102296

2022

Dual roles of oxostephanine as an Aurora kinase inhibitor and angiogenesis suppressor.

Applications

Unspecified application

Species

Unspecified reactive species

Thu-Hien Thi Tran,Le-Duy Ba Vu,Huy Quoc Nguyen,Hanh Bich Pham,Xuan-Phuong Thi Do,Uyen Thi Trang Than,Thu-Huong Thi Pham,Linh Dieu Do,Kim-Van Thi Le,Thao Phuong Nguyen,My-Nhung Thi Hoang

Cell proliferation 55:e13288 PubMed35768997

2022

Peripubertal requirement of Tsg101 in maintaining the integrity of membranous structures in mouse oocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Hyejin Shin,Dayoung Park,Jiyeon Kim,Min-Yeong Nam,Sojung Kwon,Da-Eun Um,Ji-Eun Oh,Esther Youn,Yhong-Hee Shim,Kay-Uwe Wagner,Jin Hyun Jun,Hye-Ryun Kim,Haengseok Song,Hyunjung Jade Lim

Nature structural & molecular biology 28:989-996 PubMed34811519

2021

Structure of the human SAGA coactivator complex.

Applications

Unspecified application

Species

Unspecified reactive species

Dominik A Herbst,Meagan N Esbin,Robert K Louder,Claire Dugast-Darzacq,Gina M Dailey,Qianglin Fang,Xavier Darzacq,Robert Tjian,Eva Nogales

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H3 (phospho S10) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (phospho S10) antibody (AB183626)

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (phospho S10) antibody (AB183626)

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (phospho S10) antibody (AB183626)

Immunoprecipitation - Anti-Histone H3 (phospho S10) antibody (AB183626)

ChIP - Anti-Histone H3 (phospho S10) antibody (AB183626)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (phospho S10) antibody (AB183626)

Western blot - Anti-Histone H3 (phospho S10) antibody (AB183626)

Western blot - Anti-Histone H3 (phospho S10) antibody (AB183626)

IHC - Wholemount - Anti-Histone H3 (phospho S10) antibody (AB183626)

IHC - Wholemount - Anti-Histone H3 (phospho S10) antibody (AB183626)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (5)

Product promise