AB222775

Anti-Histone H3 (phospho T3) antibody [RM159]

Recombinant
각 태그의 뜻

Be the first to review this product! 리뷰 제출

(1 출판물)

Rabbit Recombinant Monoclonal H3 phospho T3 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human H3C1 phospho T3.

대체 명칭 보기

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3FC, HIST1H3C, H3C1, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3T3p

3 이미지

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (phospho T3) antibody [RM159] (AB222775)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (phospho T3) antibody [RM159] (AB222775)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Histone H3 (red) using ab222775 at 2 μg/ml in ICC/IF.

Actin filaments were labeled with fluorescein phalloidin (green).

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (phospho T3) antibody [RM159] (AB222775)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Histone H3 (red) using ab222775 at 2 μg/ml in ICC/IF.

Actin filaments were labeled with fluorescein phalloidin (green).

Supplier Data

Western blot - Anti-Histone H3 (phospho T3) antibody [RM159] (AB222775)

All lanes:

Western blot - Anti-Histone H3 (phospho T3) antibody [RM159] (ab222775) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate, non-treated

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) treated with Nocodazole

Lane 3:

Recombinant Histone H3.3

Predicted band size: 15 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

RM159

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human H3C1 phospho T3. The exact immunogen used to generate this antibody is proprietary information.

P68431

Specificity

ab222775 reacts to Histone H3 phosphorylated at Threonine 3. No cross reactivity with other phosphorylated histones

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.5-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

style

left-column

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

Purification 관련 사항

ab222775 was purified from an animal origin–free culture supernatant.

보관 버퍼

Preservative: 0.09% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

See full target information H3C1 phospho T3

style

left-column

제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Experimental and therapeutic medicine 22:1271 PubMed34594408

2021

TFEC contributes to cardiac hypertrophy by inhibiting AMPK/mTOR signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Zhao,Zhenyu Wang,Yehong Chi,Chunmei Ni,Xudan Zheng

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H3 (phospho T3) antibody [RM159]

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (phospho T3) antibody [RM159] (AB222775)

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (phospho T3) antibody [RM159] (AB222775)

Western blot - Anti-Histone H3 (phospho T3) antibody [RM159] (AB222775)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise