AB238310

Anti-Histone H3 (unmodified K4) antibody [RM186]

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Rabbit Monoclonal H3 unmod K4 antibody. N-terminal. Suitable for ELISA, WB, ICC/IF and reacts with Synthetic peptide, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human Histone H3.1 unmod K4.

대체 명칭 보기

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l

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Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (unmodified K4) antibody [RM186] (AB238310)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (unmodified K4) antibody [RM186] (AB238310)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells treated with sodium butyrate stained for Histone H3 (unmodified K4) (red) using ab238310 at 2 μg/ml in ICC/IF. Actin filaments have been labeled with fluorescein phalloidin (green).``

Supplier Data

Western blot - Anti-Histone H3 (unmodified K4) antibody [RM186] (AB238310)

All lanes:

Western blot - Anti-Histone H3 (unmodified K4) antibody [RM186] (ab238310) at 1 µg/mL

Lane 1:

Acid extracts of HeLa (human epithelial cell line from cervix adenocarcinoma) cells treated with sodium butyrate

Lane 2:

Acid extracts of HeLa cells treated with Nocodazole

Lane 3:

Acid extracts of HeLa cells

Lane 4:

Recombinant Histone H3.1

Predicted band size: 15 kDa

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ELISA - Anti-Histone H3 (unmodified K4) antibody [RM186] (AB238310)

ELISA

Supplier Data

ELISA - Anti-Histone H3 (unmodified K4) antibody [RM186] (AB238310)

ab238310 specifically recognizes Histone H3 unmodified at Lys4 and does not recognize acetylated, mono-methylated, dimethylated, or trimethylated Lys4. The antibody binding specificity allows for modifications of Arg2 or Thr3 in histone H3.``

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

RM186

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB, ELISA

applications

Immunogen

Synthetic Peptide within Human Histone H3.1 unmod K4. The exact immunogen used to generate this antibody is proprietary information.

P68431

Specificity

ab238310 recognizes Histone H3 unmodified at Lys4 and does not recognize acetylated or methylated Lys4. The antibody binding specificity allows for modifications of Arg2, Thr3, and/or other modifications in Histone H3.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.5-2 µg/mL", "ICCIF-species-notes": "" }, "Synthetic peptide": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

Purification 관련 사항

Purified from an animal origin–free culture supernatant.

보관 버퍼

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

See full target information Histone H3.1 unmod K4

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of cachexia, sarcopenia and muscle 16:e13734 PubMed39971710

2025

SIRT6 Ameliorates Cancer Cachexia-Associated Adipose Wasting by Suppressing TNFR2 Signalling in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Kang Xu,Yida Wang,Fang Wang,Yannan Guo,Yu Ren,Vivien Low,Sungyun Cho,Qingfei Liu,Ying Qiu,Xue Li,Kang Yu,Zhongchi Li,Zhao Wang

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H3 (unmodified K4) antibody [RM186]

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (unmodified K4) antibody [RM186] (AB238310)

Western blot - Anti-Histone H3 (unmodified K4) antibody [RM186] (AB238310)

ELISA - Anti-Histone H3 (unmodified K4) antibody [RM186] (AB238310)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise