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AB240615

Anti-Histone H4 (butyryl K16) antibody

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Rabbit Polyclonal Histone H4 butyryl K16 antibody. Suitable for ChIP, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human H4C1 buK16.

대체 명칭 보기

H4/A, H4FA, HIST1H4A, H4C2, H4/I, H4FI, HIST1H4B, H4C3, H4/G, H4FG, HIST1H4C, H4C4, H4/B, H4FB, HIST1H4D, H4C5, H4/J, H4FJ, HIST1H4E, H4C6, H4/C, H4FC, HIST1H4F, H4C8, H4/H, H4FH, HIST1H4H, H4C9, H4/M, H4FM, HIST1H4I, H4C11, H4/E, H4FE, HIST1H4J, H4C12, H4/D, H4FD, HIST1H4K, H4C13, H4/K, H4FK, HIST1H4L, H4C14, H4/N, H4F2, H4FN, HIST2H4, HIST2H4A, H4C15, H4/O, H4FO, HIST2H4B, H4C16, H4-16, HIST4H4, H4C1, Histone H4, H4K16bu

2 이미지
Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (butyryl K16) antibody (AB240615)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (butyryl K16) antibody (AB240615)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells (treated with 30 mM sodium butyrate for 4 hours) labeling Histone H4 (butyryl K16) with ab240615 at 1/50 dilution in ICC/IF.

The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

ChIP - Anti-Histone H4 (butyryl K16) antibody (AB240615)
  • ChIP

Supplier Data

ChIP - Anti-Histone H4 (butyryl K16) antibody (AB240615)

HeLa (human epithelial cell line from cervix adenocarcinoma; 4 x 106, treated with 30mM sodium butyrate for 4 hours) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5 μg ab240615 or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, ChIP, WB

applications

Immunogen

Synthetic Peptide within Human H4C1 buK16. The exact immunogen used to generate this antibody is proprietary information.

P62805

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대체품

Primary Antibodies

AB177190

Anti-Histone H4 (tri methyl K20) antibody [EPR17001(2)] - ChIP Grade

20ul selling size|RabMAb|Advanced Validation|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (tri methyl K20) antibody [EPR17001(2)] - ChIP Grade (AB177190)

  • 5
  • 5
Primary Antibodies

AB177840

Anti-Histone H4 antibody [EPR16599] - ChIP Grade

RabMAb|Advanced Validation|Recombinant|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 antibody [EPR16599] - ChIP Grade (AB177840)

  • 4
  • 4
Primary Antibodies

AB45166

Anti-Histone H4 (acetyl K8) antibody [EP1002Y] - ChIP Grade

RabMAb|Advanced Validation|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K8) antibody [EP1002Y] - ChIP Grade (AB45166)

  • 5
  • 4
Primary Antibodies

AB177790

Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606]

20ul selling size|RabMAb|Advanced Validation|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] (AB177790)

  • 5
  • 2
Primary Antibodies

AB177793

Anti-Histone H4 (acetyl K12) antibody [EPR17906]

20ul selling size|RabMAb|Advanced Validation|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K12) antibody [EPR17906] (AB177793)

  • 5
  • 1
Primary Antibodies

AB51997

Anti-Histone H4 (acetyl K5) antibody [EP1000Y] - ChIP Grade

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K5) antibody [EP1000Y] - ChIP Grade (AB51997)

  • 5
  • 9
Primary Antibodies

AB109463

Anti-Histone H4 (acetyl K16) antibody [EPR1004]

20ul selling size|RabMAb|Advanced Validation|Recombinant

Flow Cytometry (Intracellular) - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (AB109463)

  • 5
  • 15

보완 제품

Primary Antibodies

AB11751

Anti-alpha 1 Spectrin antibody [17C7]

Western blot - Anti-alpha 1 Spectrin antibody [17C7] (AB11751)

  • 4
  • 3
Primary Antibodies

AB68703

Anti-ALK-1 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALK-1 antibody (AB68703)

  • 4
  • 1
Primary Antibodies

AB16667

Anti-Ki67 antibody [SP6]

BOND RX™ Validated|KO Validated|RabMAb|Advanced Validation|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] (AB16667)

  • 5
  • 133
Primary Antibodies

AB905

Anti-Mycobacterium tuberculosis antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mycobacterium tuberculosis antibody (AB905)

  • 4
  • 2
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "<p>Use 5 μg for 4 x 10<sup>6</sup> cells.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
보관 버퍼
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
See full target information H4C1 buK16
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nucleic acids research 50:6190-6210 PubMed35687128

2022

Inhibition of histone acetyltransferase GCN5 by a transcription factor FgPacC controls fungal adaption to host-derived iron stress.

Applications

Unspecified application

Species

Unspecified reactive species

Qin Gu,Yujie Wang,Xiaozhen Zhao,Bingqin Yuan,Mengxuan Zhang,Zheng Tan,Xinyue Zhang,Yun Chen,Huijun Wu,Yuming Luo,Nancy P Keller,Xuewen Gao,Zhonghua Ma
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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